A possible mechanism for island formation by rat ascites hepatoma cells with special reference to the function of aggregation factor at the cell surface

1979 ◽  
Vol 93 (2) ◽  
pp. 123-136 ◽  
Author(s):  
Y. Ishimaru ◽  
K. Kudo ◽  
Y. Koga ◽  
H. Hayashi
Keyword(s):  
Cell Surface ◽  
Special Reference ◽  
Hepatoma Cells ◽  
Ascites Hepatoma ◽  
Island Formation ◽  
Aggregation Factor ◽  
Rat Ascites Hepatoma

Cell surface heparan sulphate and adhesive property of sublines of rat ascites hepatoma AH7974

1988 ◽  
Vol 90 (4) ◽  
pp. 683-689 ◽  
Author(s):  
A. Kimura ◽  
T. Kawaguchi ◽  
T. Ono ◽  
A. Sakuma ◽  
Y. Yokoya ◽  
...  
Keyword(s):  
Cell Surface ◽  
Culture Medium ◽  
Heparan Sulphate ◽  
Soluble Form ◽  
Foetal Calf Serum ◽  
Serum Free Medium ◽  
Free Medium ◽  
Ascites Hepatoma ◽  
Serum Free ◽  
Rat Ascites Hepatoma

Two variants (74AD and 74FL) established from rat ascites hepatoma AH7974 were examined for the production of glycosaminoglycans in culture. There was no difference between the adhesive (74AD) and the floating (74FL) variants in quantity of glycosaminoglycans produced by their cultivation in minimum essential medium supplemented with 10% foetal calf serum. However, they were distinctly different in the distribution patterns of heparan sulphate. In 74FL, about 70% of total heparan sulphate was found in the culture medium in soluble form, whereas in 74AD, only 7% was found in the medium and the rest was in the cell-substratum complex. In a serum-free medium, 74AD cells grew without adhering to the substratum. After cultivation, more than 90% of total heparan sulphate was found in the cell-associated fractions and the rest in the substratum fractions. No heparan sulphate was detected in the culture medium. On the other hand, 74FL cells released heparan sulphate to the serum-free medium as much as to the serum-containing medium. The increase in amount of heparan sulphate in the culture medium of 74FL cells was supposed to be caused by failure of the cells to deposit heparan sulphate at the cell surface and not caused by increased production. Cell-substratum adhesion mechanisms involving cell surface heparan sulphate (heparan sulphate proteoglycan) and some serum intermediate(s) are discussed for 74AD cells.

scholarly journals Loss of hormonal control of glycogenolysis in rat ascites hepatoma cells

1986 ◽  
Vol 40 ◽  
pp. 107
Author(s):  
Kenichi Miyamoto ◽  
Shinya Wakusawa ◽  
Toshikazu Yanaoka ◽  
Ryozo Koshiura
Keyword(s):  
Hepatoma Cells ◽  
Hormonal Control ◽  
Ascites Hepatoma ◽  
Rat Ascites Hepatoma

scholarly journals Purification and characterization of nuclear alkaline phospholipase A2 in rat ascites hepatoma cells

FEBS Letters ◽  
1996 ◽  
Vol 394 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Takashi Oishi ◽  
Keiko Tamiya-Koizumi ◽  
Ichiro Kudo ◽  
Satoshi Iino ◽  
Kenzo Takagi ◽  
...  
Keyword(s):  
Phospholipase A2 ◽  
Hepatoma Cells ◽  
Ascites Hepatoma ◽  
Purification And Characterization ◽  
Rat Ascites Hepatoma

Cell membrane polarity in rat ascites hepatoma cells

1985 ◽  
Vol 240 (2) ◽  
Author(s):  
Yasuji Ishimaru ◽  
Ryuichi Hattori ◽  
Chen Jinyan ◽  
Hideo Hayashi
Keyword(s):  
Cell Membrane ◽  
Hepatoma Cells ◽  
Ascites Hepatoma ◽  
Rat Ascites Hepatoma

Biological and Structural Differences between tRNAval Species Isolated from Rat Ascites Hepatoma Cells and Normal Rat Liver1

1981 ◽  
Vol 90 (2) ◽  
pp. 535-544 ◽  
Author(s):  
Nobuko SHINDO-OKADA ◽  
Yoshiyuki KUCHINO ◽  
Fumio HARADA ◽  
Norihiro OKADA ◽  
Susumu NISHIMURA
Keyword(s):  
Hepatoma Cells ◽  
Ascites Hepatoma ◽  
Structural Differences ◽  
Normal Rat ◽  
Rat Ascites Hepatoma

Cell surface laminin-like substances and laminin-related carbohydrates of rat ascites hepatoma AH7974 and its variants with different lung-colonizing potential

1994 ◽  
Vol 12 (3) ◽  
pp. 203-212 ◽  
Author(s):  
Takanori Kawaguchi ◽  
Tomohisa Ono ◽  
Hironao Wakabayashi ◽  
Seiji Igarashi
Keyword(s):  
Cell Surface ◽  
Ascites Hepatoma ◽  
Rat Ascites Hepatoma

scholarly journals Putrescine-stimulated Intracellular Ca2+Release for Invasiveness of Rat Ascites Hepatoma Cells

1998 ◽  
Vol 89 (1) ◽  
pp. 67-75 ◽  
Author(s):  
Yoshiyuki Ashida ◽  
Akemichi Ueno ◽  
Yoshihiro Miwa ◽  
Keiko Miyoshi ◽  
Hideo Inoue
Keyword(s):  
Hepatoma Cells ◽  
Ascites Hepatoma ◽  
Rat Ascites Hepatoma
Sign in / Sign up

Export Citation Format

Share Document