Glass bead method for releasing contact inhibition

1977 ◽  
Vol 3 (1) ◽  
pp. 551-552
Author(s):  
Gerald M. Kolodny
Keyword(s):  
Glass Bead ◽  
Contact Inhibition ◽  
Bead Method

scholarly journals XRF determination of major elements in rocks using glass bead method with synthetic standard samples.

1988 ◽  
Vol 37 (11) ◽  
pp. 595-600 ◽  
Author(s):  
Akira YOSHIOKA ◽  
Takao SEKINE ◽  
Hidemistu YOSHIDA ◽  
Koichi NOMURA
Keyword(s):  
Glass Bead ◽  
Major Elements ◽  
Standard Samples ◽  
Synthetic Standard ◽  
Bead Method

Adhesiveness of 51Cr-Labelled Platelets

1979 ◽  
Author(s):  
J. Dale ◽  
E. Myhre
Keyword(s):  
Valve Replacement ◽  
Glass Bead ◽  
Heart Valves ◽  
Prosthetic Heart Valves ◽  
Platelet Adhesiveness ◽  
Labelling Procedure ◽  
Bead Method

The procedure of labelling platelets may disturb their reactivity, and methods based on measurements of radioactivity may therefore not reflect the survival of representative platelets. To study this, nine patients with prosthetic heart valves were chosen because platelet adhesiveness is reduced to a varying degree after valve replacement. Autologous platelets were labelled by incubation of PRP from 120 ml of ACD-blood with 250 μci 51CrO4 for one hour, and then reinjected. After 16 hours, platelet retention was estimated according to Hellem’s modified glass bead method, in parallel samples, the retention of radioactivity was measured. Labelled and non-labelled platelets were retained in the glass-bead columns to the same degree, indicating that the Labelling procedure did not permanently disturb platelet adhesiveness.

A glass bead method for picking bacterial colonies

1997 ◽  
Vol 2 (1) ◽  
pp. 138-139
Author(s):  
Bruce L. Granger
Keyword(s):  
Glass Bead ◽  
Bead Method

The Effect of Dipyridamole and RA 233 on Human Platelet Function in Vitro

1973 ◽  
Vol 29 (03) ◽  
pp. 694-700 ◽  
Author(s):  
Paul L. Rifkin ◽  
Marjorie B. Zucker
Keyword(s):  
Mechanism Of Action ◽  
Human Blood ◽  
Glass Bead ◽  
Heart Valves ◽  
Human Platelet ◽  
Drug Effects ◽  
Simple Relation ◽  
Prosthetic Heart Valves ◽  
Induced Aggregation

SummaryDipyridamole (Persantin) is reported to prolong platelet survival and inhibit embolism in patients with prosthetic heart valves, but its mechanism of action is unknown. Fifty jxM dipyridamole failed to reduce the high percentage of platelets retained when heparinized human blood was passed through a glass bead column, but prolonged the inhibition of retention caused by disturbing blood in vitro. Possibly the prostheses act like disturbance. Although RA 233 was as effective as dipyridamole in inhibiting the return of retention, it was less effective in preventing the uptake of adenosine into erythrocytes, and more active in inhibiting ADP-induced aggregation and release. Thus there is no simple relation between these drug effects.

Inhibition of Human and Animal Platelet Adhesiveness to Glass Bead Columns by Adenosine, Dipyridamole, Chlorpromazine and Acetylsalicylic Acid

1976 ◽  
Vol 36 (02) ◽  
pp. 401-410 ◽  
Author(s):  
Buichi Fujttani ◽  
Toshimichi Tsuboi ◽  
Kazuko Takeno ◽  
Kouichi Yoshida ◽  
Masanao Shimizu
Keyword(s):  
Guinea Pig ◽  
Acetylsalicylic Acid ◽  
Guinea Pigs ◽  
Glass Bead ◽  
Animal Species ◽  
Inhibitory Effect ◽  
Rabbit Platelet ◽  
Platelet Adhesiveness

SummaryThe differences among human, rabbit and guinea-pig platelet adhesiveness as for inhibitions by adenosine, dipyridamole, chlorpromazine and acetylsalicylic acid are described, and the influence of measurement conditions on platelet adhesiveness is also reported. Platelet adhesiveness of human and animal species decreased with an increase of heparin concentrations and an increase of flow rate of blood passing through a glass bead column. Human and rabbit platelet adhesiveness was inhibited in vitro by adenosine, dipyridamole and chlorpromazine, but not by acetylsalicylic acid. On the other hand, guinea-pig platelet adhesiveness was inhibited by the four drugs including acetylsalicylic acid. In in vivo study, adenosine, dipyridamole and chlorpromazine inhibited platelet adhesiveness in rabbits and guinea-pigs. Acetylsalicylic acid showed the inhibitory effect in guinea-pigs, but not in rabbits.

Non-Thrombogenicity of Clean Glass Revealed by Native Whole Blood Assay in Bead Columns

1983 ◽  
Vol 50 (04) ◽  
pp. 814-820 ◽  
Author(s):  
J A Bergeron ◽  
J M DiNovo ◽  
A F Razzano ◽  
W J Dodds
Keyword(s):  
Whole Blood ◽  
Glass Bead ◽  
Factor Xii ◽  
Platelet Factor ◽  
Serotonin Content ◽  
Whole Blood Assay ◽  
Vascular Integrity ◽  
Blood Assay ◽  
Standard Contact ◽  
Recalcification Time

SummaryThe previously described native whole blood assay for materials in solution or suspension has been adapted to materials in a bead column configuration. These experiments showed that the glass itself accounts for little or none of the high blood-reactivity observed with conventional glass bead columns. Columns composed solely of soft glass that was “cleaned” by heat treatment (500-595° C 18 hr, electric oven) were benign toward flowing native whole blood for all variables measured (platelet count and platelet-free plasma [C14]-serotonin content, platelet factor 3 and factor XII activities, and recalcification time) with the standard contact protocol. In addition, the effluent successfully maintained perfusion of the isolated kidney, a measure of the ability of platelets to support vascular integrity. Prolonged (30 min) normothermic contact with titrated whole blood increased the subsequent reactivity of initially clean glass toward whole blood albeit to a level much less than that of conventional glass bead columns.

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