Dye affinity chromatography of ricin subunits

1986 ◽  
Vol 6 (12) ◽  
pp. 1035-1040 ◽  
Author(s):  
Simonetta Sperti ◽  
Lucio Montanaro ◽  
Fioretta Rambelli
Keyword(s):  
Affinity Chromatography ◽  
Simple Method ◽  
Blue Dextran ◽  
A Chain ◽  
Dye Affinity Chromatography ◽  
Blue Sepharose

A rapid and simple method for the isolation of the two chains of ricin is described. The method involves two chromatographic runs, the first on blue-Sepharose and the second on blue dextran-Sepharose. It is moreover shown that the A chain of ricin, but not the B chain, binds to poly(U)-Sepharose.

Polymer displacement in dye-affinity chromatography

1995 ◽  
Vol 710 (2) ◽  
pp. 259-266 ◽  
Author(s):  
Igor Yu. Galaev ◽  
Pär Arvidsson ◽  
Bo Mattiasson
Keyword(s):  
Affinity Chromatography ◽  
Dye Affinity Chromatography

Fungal pectic enzyme fractionation by dye affinity chromatography

1996 ◽  
Vol 10 (3) ◽  
pp. 211-214 ◽  
Author(s):  
Silvia A. Camperi ◽  
Mariano Grasselli ◽  
Osvaldo Cascone
Keyword(s):  
Affinity Chromatography ◽  
Pectic Enzyme ◽  
Dye Affinity Chromatography

scholarly journals Nickel Ferrite Nanoparticles as an Adsorbent for Immobilized Metal Affinity Chromatography of Proteins

2020 ◽  
Author(s):  
Kayambu Kannan ◽  
Joyeeta Mukherjee ◽  
Prashant Mishra ◽  
Munishwar N Gupta
Keyword(s):  
Magnetic Material ◽  
Affinity Chromatography ◽  
Nickel Ferrite ◽  
High Capacity ◽  
Ferrite Nanoparticles ◽  
Immobilized Metal Affinity Chromatography ◽  
Simple Method ◽  
Metal Affinity ◽  
Nickel Ferrite Nanoparticles ◽  
Ir Transmission

Abstract A simple method of preparing amorphous nickel ferrite nanoparticles of about 5 nm diameter is described. These particles were characterized by dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM) and selected area electron diffraction (SAED). The nanoparticles were evaluated for their use as a magnetic material for immobilized metal affinity chromatography (IMAC). The ferrite nanoparticles bound to bovine serum albumin (BSA) and the binding fitted Langmuir isotherm model. A high capacity of 916 mg BSA/g dried nanoparticle was observed. Six proteins (Soybean trypsin inhibitor (STI), lactate dehydrogenase (LDH), papain, catalase, β-galactosidase and casein) were used and all were found to bind at >90% level (except papain which showed 84% binding). All the proteins except LDH and β-galactosidase could be eluted with 1 M imidazole and with % activity recovery of >80%. Papain could be purified from its dried crude latex by 5-fold and purified papain showed a single band on SDS-PAGE. These nanoparticles constitute a high capacity and are magnetic material useful for IMAC and do not require any pre-functionalization.

Coated silica and its behaviour in dye-affinity chromatography

1991 ◽  
Vol 543 ◽  
pp. 9-16 ◽  
Author(s):  
Danica Mislovičová ◽  
Ivan Novák ◽  
Mikuláš Pašteka
Keyword(s):  
Affinity Chromatography ◽  
Dye Affinity Chromatography
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