Mimesis of peroxidase by Mn-TMPyP in the catalytic fluorescence reaction of the homovanillic acid-hydrogen peroxide system

1990 ◽  
Vol 100 (1-2) ◽  
pp. 63-68 ◽  
Author(s):  
Yun -Xiang Ci ◽  
Fang Wang
1973 ◽  
Vol 19 (11) ◽  
pp. 1296-1299 ◽  
Author(s):  
Carl W Seiter ◽  
Hoyle D Hill ◽  
George K Summer

Abstract We describe a sensitive automated fluorometric method for glucose in whole blood spotted on filter paper. By action of glucose oxidase (EC 1.1.3.4) on glucose, hydrogen peroxide is produced, which then reacts with 4-hydroxy-3-methoxy-phenylacetic acid (homovanillic acid) in the presence of horseradish peroxidase (EC 1.11.1.7) to form a fluorescent condensation product. Obtained at a rate of 60 samples per hour, the results are accurate, linear, and reproducible. Whole blood glucose can be determined by this method with an error (CV) of less than 5%.


1990 ◽  
Vol 23 (11) ◽  
pp. 2107-2122 ◽  
Author(s):  
Itsuo Mori ◽  
Yoshikazu Fujita ◽  
Minako Toyoda ◽  
Keiji Kato ◽  
Masao Akagi

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