Protection of mice against virulent virus infection by a temperature-sensitive mutant derived from an HVJ (Sendai virus) carrier culture

1979 ◽  
Vol 61 (4) ◽  
pp. 297-304 ◽  
Author(s):  
Y. Kimura ◽  
H. Aoki ◽  
K. Shimokata ◽  
Y. Ito ◽  
Michiko Takano ◽  
...  
Keyword(s):  
Virus Infection ◽  
Sendai Virus ◽  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant ◽  
Virulent Virus ◽  
Virus Carrier

scholarly journals Homologous Interference Induced by a Temperature-sensitive Mutant Derived from an HVJ (Sendai Virus) Carrier Culture

1976 ◽  
Vol 33 (2) ◽  
pp. 333-343 ◽  
Author(s):  
Y. Kimura ◽  
E. Norrby ◽  
I. Nagata ◽  
Y. Ito ◽  
K. Shimokata ◽  
...  
Keyword(s):  
Sendai Virus ◽  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant ◽  
Virus Carrier

Immediate protection of mice from lethal wild-type Sendai virus (HVJ) infections by a temperature-sensitive mutant, HVJpi, possessing homologous interfering capacity

Virology ◽  
1990 ◽  
Vol 177 (1) ◽  
pp. 65-74 ◽  
Author(s):  
Katsuhiro Kiyotani ◽  
Shinichi Takao ◽  
Takemasa Sakaguchi ◽  
Tetsuya Yoshida
Keyword(s):  
Sendai Virus ◽  
Temperature Sensitive ◽  
Wild Type ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant

Aerosol Vaccination with a Sendai Virus Temperature-Sensitive Mutant (HVJ-pB) Derived from Persistently Infected Cells

1990 ◽  
Vol 162 (2) ◽  
pp. 402-407 ◽  
Author(s):  
H. Iwata ◽  
M. Thgaya ◽  
K. Matsumoto ◽  
T. Miyadai ◽  
T. Yokochi ◽  
...  
Keyword(s):  
Sendai Virus ◽  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant ◽  
Persistently Infected ◽  
Infected Cells

scholarly journals Nontransmissible Virus-Like Particle Formation by F-Deficient Sendai Virus Is Temperature Sensitive and Reduced by Mutations in M and HN Proteins

2003 ◽  
Vol 77 (5) ◽  
pp. 3238-3246 ◽  
Author(s):  
Makoto Inoue ◽  
Yumiko Tokusumi ◽  
Hiroshi Ban ◽  
Takumi Kanaya ◽  
Tsuyoshi Tokusumi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Sendai Virus ◽  
M Protein ◽  
Temperature Sensitive ◽  
Amino Acid Substitutions ◽  
Wild Type ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant ◽  
Virus Like Particle ◽  
Infected Cells

ABSTRACT The formation of nontransmissible virus-like particles (NTVLP) by cells infected with F-deficient Sendai virus (SeV/ΔF) was found to be temperature sensitive. Analysis by hemagglutination assays and Western blotting demonstrated that the formation of NTVLP at 38°C was about 1/100 of that at 32°C, whereas this temperature-sensitive difference was only moderate in the case of F-possessing wild-type SeV. In order to reduce the NTVLP formation with the aim of improving SeV for use as a vector for gene therapy, amino acid substitutions found in temperature-sensitive mutant SeVs were introduced into the M (G69E, T116A, and A183S) and HN (A262T, G264R, and K461G) proteins of SeV/ΔF to generate SeV/MtsHNtsΔF. The use of these mutations allows vector production at low temperature (32°C) and therapeutic use at body temperature (37°C) with diminished NTVLP formation. As expected, the formation of NTVLP by SeV/MtsHNtsΔF at 37°C was decreased to about 1/10 of that by SeV/ΔF, whereas the suppression of NTVLP formation did not cause either enhanced cytotoxicity or reduced gene expression of the vector. The vectors showed differences with respect to the subcellular distribution of M protein in the infected cells. Clear and accumulated immunocytochemical signals of M protein on the cell surface were not observed in cells infected by SeV/ΔF at an incompatible temperature, 38°C, or in those infected by SeV/MtsHNtsΔF at 37 or 38°C. The absence of F protein in SeV/ΔF and the additional mutations in M and HN in SeV/MtsHNtsΔF probably weaken the ability to transport M protein to the plasma membrane, leading to the diminished formation of NTVLP.

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