A role for alanine in the ammonium regulation of cephalosporin biosynthesis inStreptomyces clavuligerus

1994 ◽  
Vol 13 (4) ◽  
pp. 217-219 ◽  
Author(s):  
Suseela Kasarenini ◽  
Arnold L. Demain
Keyword(s):  
Ammonium Regulation

Effect of inactivation of genes involved in ammonium regulation on the biohydrogen production of Rhodobacter capsulatus

2011 ◽  
Vol 36 (21) ◽  
pp. 13536-13546 ◽  
Author(s):  
Gülşah Pekgöz ◽  
Ufuk Gündüz ◽  
Inci Eroğlu ◽  
Meral Yücel ◽  
Kornél Kovács ◽  
...  
Keyword(s):  
Rhodobacter Capsulatus ◽  
Biohydrogen Production ◽  
Ammonium Regulation

Ammonium regulation inSaccharopolyspora erythraea. Part I: Growth and antibiotic production

1994 ◽  
Vol 16 (1) ◽  
pp. 63-68 ◽  
Author(s):  
Jean Potvin ◽  
Paul P�ringer
Keyword(s):  
Antibiotic Production ◽  
Ammonium Regulation

Glutamine synthetase and ammonium regulation of nitrogenase synthesis in Klebsiella

Nature ◽  
1974 ◽  
Vol 251 (5475) ◽  
pp. 481-485 ◽  
Author(s):  
Roy S. Tubb
Keyword(s):  
Glutamine Synthetase ◽  
Ammonium Regulation

Ammonium regulation of urate uptake in Chlamydomonas reinhardtii

Planta ◽  
1987 ◽  
Vol 171 (4) ◽  
pp. 496-500 ◽  
Author(s):  
Manuel Pineda ◽  
Purificaci�n Cabello ◽  
Jacobo C�rdenas
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Ammonium Regulation

scholarly journals Ammonium Regulation in Aspergillus nidulans

1973 ◽  
Vol 114 (3) ◽  
pp. 943-950 ◽  
Author(s):  
J. A. Pateman ◽  
J. R. Kinghorn ◽  
Etta Dunn ◽  
E. Forbes
Keyword(s):  
Aspergillus Nidulans ◽  
Ammonium Regulation

The effect of the carbon source on ammonium regulation in Aspergillus nidulans

1974 ◽  
Vol 128 (1) ◽  
pp. 95-98 ◽  
Author(s):  
J. R. Kinghorn ◽  
J. A. Pateman
Keyword(s):  
Carbon Source ◽  
Aspergillus Nidulans ◽  
Ammonium Regulation

Ammonium regulation inSaccharopolyspora erythraea. Part II: Regulatory effects under different nutritional conditions

1994 ◽  
Vol 16 (1) ◽  
pp. 69-74 ◽  
Author(s):  
Jean Potvin ◽  
Paul P�ringer
Keyword(s):  
Nutritional Conditions ◽  
Ammonium Regulation ◽  
Regulatory Effects

The glnAntrBC operon of Herbaspirillum seropedicae is transcribed by two oppositely regulated promoters upstream of glnA

2007 ◽  
Vol 53 (1) ◽  
pp. 100-105 ◽  
Author(s):  
Stefan Schwab ◽  
Emanuel M Souza ◽  
Marshall G Yates ◽  
Darlene C Persuhn ◽  
M Berenice R. Steffens ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Endophytic Bacterium ◽  
Wild Type ◽  
Herbaspirillum Seropedicae ◽  
Promoter Sequences ◽  
Intergenic Regions ◽  
In The Wild ◽  
Ammonium Regulation ◽  
Regulated Promoters ◽  
Wild Type Promoter

Herbaspirillum seropedicae is an endophytic bacterium that fixes nitrogen under microaerophilic conditions. The putative promoter sequences glnAp1 (σ70-dependent) and glnAp2 (σ54), and two NtrC-binding sites were identified upstream from the glnA, ntrB and ntrC genes of this microorganism. To study their transcriptional regulation, we used lacZ fusions to the H. seropedicae glnA gene, and the glnA-ntrB and ntrB-ntrC intergenic regions. Expression of glnA was up-regulated under low ammonium, but no transcription activity was detected from the intergenic regions under any condition tested, suggesting that glnA, ntrB and ntrC are co-transcribed from the promoters upstream of glnA. Ammonium regulation was lost in the ntrC mutant strain. A point mutation was introduced in the conserved –25/–24 dinucleotide (GG→TT) of the putative σ54-dependent promoter (glnAp2). Contrary to the wild-type promoter, glnA expression with the mutant glnAp2 promoter was repressed in the wild-type strain under low ammonium levels, but this repression was abolished in an ntrC background. Together our results indicate that the H. seropedicae glnAntrBC operon is regulated from two functional promoters upstream from glnA, which are oppositely regulated by the NtrC protein.Key words: Herbaspirillum seropedicae, nitrogen assimilation, glnAntrBC operon, transcriptional regulation.

scholarly journals Yeast Two-Hybrid Studies on Interaction of Proteins Involved in Regulation of Nitrogen Fixation in the Phototrophic Bacterium Rhodobacter capsulatus

2003 ◽  
Vol 185 (17) ◽  
pp. 5240-5247 ◽  
Author(s):  
Alice Pawlowski ◽  
Kai-Uwe Riedel ◽  
Werner Klipp ◽  
Petra Dreiskemper ◽  
Silke Groß ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Rhodobacter Capsulatus ◽  
Genomic Library ◽  
Fine Tuning ◽  
Yeast Two Hybrid ◽  
Putative Protein ◽  
Yeast Two Hybrid System ◽  
Ammonium Regulation ◽  
Two Hybrid ◽  
New Protein

ABSTRACT Rhodobacter capsulatus contains two PII-like proteins, GlnB and GlnK, which play central roles in controlling the synthesis and activity of nitrogenase in response to ammonium availability. Here we used the yeast two-hybrid system to probe interactions between these PII-like proteins and proteins known to be involved in regulating nitrogen fixation. Analysis of defined protein pairs demonstrated the following interactions: GlnB-NtrB, GlnB-NifA1, GlnB-NifA2, GlnB-DraT, GlnK-NifA1, GlnK-NifA2, and GlnK-DraT. These results corroborate earlier genetic data and in addition show that PII-dependent ammonium regulation of nitrogen fixation in R. capsulatus does not require additional proteins, like NifL in Klebsiella pneumoniae. In addition, we found interactions for the protein pairs GlnB-GlnB, GlnB-GlnK, NifA1-NifA1, NifA2-NifA2, and NifA1-NifA2, suggesting that fine tuning of the nitrogen fixation process in R. capsulatus may involve the formation of GlnB-GlnK heterotrimers as well as NifA1-NifA2 heterodimers. In order to identify new proteins that interact with GlnB and GlnK, we constructed an R. capsulatus genomic library for use in yeast two-hybrid studies. Screening of this library identified the ATP-dependent helicase PcrA as a new putative protein that interacts with GlnB and the Ras-like protein Era as a new protein that interacts with GlnK.

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