In situ hybridization with digoxigenin-labelled DNA probes for the detection of human papillomavirus-induced focal epithelial hyperplasia among Venezuelans

1992 ◽  
Vol 420 (4) ◽  
pp. 295-300 ◽  
Author(s):  
Gloria Premoli-de-Percoco ◽  
Ivan Galindo ◽  
José Luis Ramirez
1989 ◽  
Vol 18 (7) ◽  
pp. 419-421 ◽  
Author(s):  
Rolf-Peter Henke ◽  
Isabelle Guerin-Reverchon ◽  
Karin Milde-Langosch ◽  
Hanna Stromme Koppang ◽  
Thomas Loning

1988 ◽  
Vol 19 (3-4) ◽  
pp. 225-238 ◽  
Author(s):  
S. Syrjänen ◽  
P. Partanen ◽  
R. Mäntyjärvi ◽  
K. Syrjänen

1995 ◽  
Vol 18 (2) ◽  
pp. 105-110 ◽  
Author(s):  
Gerard J. Nuovo ◽  
Michele Margiotta ◽  
Frances Gallery ◽  
Angella Forde

1992 ◽  
Vol 34 (4) ◽  
pp. 309-314
Author(s):  
Eliane Machado Guimarães ◽  
Geraldo Brasileiro Filho ◽  
Sérgio Danilo Junho Pena

Specimens from cervical dysplasias or carcinomas and genital condylomata acuminata were retrospectively analysed by in situ hybridization (ISH) with bioti-nylated DNA probes for human papillomavirus (HPV) types 6, 11, 16 and 18. In the control group no case was positive for HPV DNA. In mild/moderate dysplasias, 4 cases (14%) were positive for HPV 6 or 11 and 2 cases (7%), for HPV 16. In the severe dysplasia/in situ carcinoma group, 9 cases (31%) showed presence of DNA of HPV types 16 or 18. Six invasive carcinomas (20%) were positive for HPV type 16 or 18. Among condylomata acuminata, 22 cases (73%) were positive for HPV types 6 or 11. In all ISH-positive cases only one viral type was detected. No correlation between HPV DNA positivity and histological findings of HPV infection was observed. Although less sensitive than some other molecular biology techniques, in situ hybridization with biotinylated DNA probes proved to be simple and useful for detecting and typing HPV in samples routinely received for histopathological analysis.


1989 ◽  
Vol 92 (5) ◽  
pp. 604-612 ◽  
Author(s):  
Giancarlo Garuti ◽  
Fausto Boselli ◽  
Andrea R. Genazzani ◽  
Sergio Silvestri ◽  
Giulio Ratti

Sign in / Sign up

Export Citation Format

Share Document