Lectin and antibody labelling of developing rat photoreceptor cells: an electron microscope immunocytochemical study

1986 ◽  
Vol 15 (2) ◽  
pp. 219-230 ◽  
Author(s):  
D. Hicks ◽  
C. J. Barnstable
Keyword(s):  
Electron Microscope ◽  
Photoreceptor Cells ◽  
Immunocytochemical Study ◽  
Developing Rat ◽  
Antibody Labelling

Mucin-Like Glycoprotein Associated with Photoreceptor Cells in the Postnatal Developing Rat Retina

2002 ◽  
Vol 34 (2) ◽  
pp. 63-69
Author(s):  
Fumiyuki Uehara ◽  
Norio Ohba ◽  
Masayuki Ozawa
Keyword(s):  
Photoreceptor Cells ◽  
Rat Retina ◽  
Developing Rat

Angiotensinogen in the developing rat fetal hindbrain and spinal cord from 18th to 20th day of gestation: An immunocytochemical study

Neuroscience ◽  
1990 ◽  
Vol 37 (2) ◽  
pp. 517-522 ◽  
Author(s):  
P.P. Sood ◽  
J.P. Richoux ◽  
M. Panigel ◽  
J. Bouhnik ◽  
R. Wegmann
Keyword(s):  
Spinal Cord ◽  
Immunocytochemical Study ◽  
Developing Rat

Periciliary structure of developing rat photoreceptor cells. A deep etch replica and freeze substitution study

1999 ◽  
Vol 48 (6) ◽  
pp. 929-935 ◽  
Author(s):  
N. Watanabe ◽  
Y. Miyake ◽  
T. Wakabayashi ◽  
J. Usukura
Keyword(s):  
Freeze Substitution ◽  
Photoreceptor Cells ◽  
Developing Rat

scholarly journals Immunocytochemical localization of opsin in the cell membrane of developing rat retinal photoreceptors.

1984 ◽  
Vol 98 (5) ◽  
pp. 1788-1795 ◽  
Author(s):  
I Nir ◽  
D Cohen ◽  
D S Papermaster
Keyword(s):  
Plasma Membrane ◽  
Outer Segment ◽  
Plasma Membranes ◽  
Photoreceptor Cells ◽  
Rod Photoreceptor ◽  
Rod Photoreceptors ◽  
Retinal Photoreceptors ◽  
Retinal Rod ◽  
Ros Formation ◽  
Developing Rat

Mature retinal rod photoreceptors sequester opsin in the disk and plasma membranes of the rod outer segment (ROS). Opsin is synthesized in the inner segment and is transferred to the outer segment along the connecting cilium that joins the two compartments. We have investigated early stages of retinal development during which the polarized distribution of opsin is established in the rod photoreceptor cell. Retinas were isolated from newborn rats, 3-21 d old, and incubated with affinity purified biotinyl-sheep anti-bovine opsin followed by avidin-ferritin. At early postnatal ages prior to the development of the ROS, opsin is labeled by antiopsin on the inner segment plasma membrane. At the fifth postnatal day, as ROS formation begins opsin was detected on the connecting cilium plasma membrane. However, the labeling density of the ciliary plasma membrane was not uniform: the proximal cilium was relatively unlabeled in comparison with the distal cilium and the ROS plasma membrane. In nearly mature rat retinas, opsin was no longer detected on the inner segment plasma membrane. A similar polarized distribution of opsin was also observed in adult human rod photoreceptor cells labeled with the same antibodies. These results suggest that some component(s) of the connecting cilium and its plasma membrane may participate in establishing and maintaining the polarized distribution of opsin.

scholarly journals Immunocytochemical localization of opsin in outer segments and Golgi zones of frog photoreceptor cells. An electron microscope analysis of cross-linked albumin-embedded retinas

1978 ◽  
Vol 77 (1) ◽  
pp. 196-210 ◽  
Author(s):  
DS Papermaster ◽  
BG Schneider ◽  
MA Zorn ◽  
JP Kraehenbuhl
Keyword(s):  
Electron Microscope ◽  
Outer Segment ◽  
Specific Binding ◽  
Photoreceptor Cells ◽  
Indirect Detection ◽  
Thin Sections ◽  
Rods And Cones ◽  
Outer Segments ◽  
Cell Structures ◽  
Cone Photopigments

Adult vertebrate retinal cells (rod and cones) continuously synthesize membrane proteins and transport them to the organelle specialized for photon capture, the outer segment. The cell structures involved in the synthesis of opsin have been identified by means of immunocytochemistry at the electron microscope level. Two indirect detection systems were used: (a) rabbit antibodies to frog opsin were localized with ferritin conjugated F(ab')2 of sheep antibodies to rabbit F(ab')2 and (b) sheep antibodies to cattle opsin were coupled to biotin and visualized by means of avidin-ferritin conjugates (AvF). The reagents were applied directly to the surface of thin sections of frog retinal tissues embedded in glutaraldehyde cross-linked bovine serum albumin (BSA). Specific binding of anti-opsin antibodies indicates that opsin is localized in the disks of rod outer segments (ROS), as expected, and in the Golgi zone of the rod cell inner segments. In addition, we observed quantitatively different labeling patterns of outer segments of rods and cones with each of the sera employed. These reactions may indicate immunological homology of rod and cone photopigments. Because these quantitiative variations of labeling density extend along the entire length of the outer segment, they also serve to identify the cell which has shed its disks into adjacent pigment ipithelial cell phagosomes.

Cholinergic amacrine cells of the chicken retina: A light and electron microscope immunocytochemical study

Neuroscience ◽  
1987 ◽  
Vol 21 (3) ◽  
pp. 725-743 ◽  
Author(s):  
T.J. Millar ◽  
I. Ishimoto ◽  
I.W. Chubb ◽  
M.L. Epstein ◽  
C.D. Johnson ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Amacrine Cells ◽  
Immunocytochemical Study ◽  
Chicken Retina ◽  
Cholinergic Amacrine Cells
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