Stimulation of active potassium transport in LK sheep red cells by blood group-L-antiserum

1970 ◽  
Vol 3 (1) ◽  
pp. 1-13 ◽  
Author(s):  
P. K. Lauf ◽  
B. A. Rasmusen ◽  
P. G. Hoffman ◽  
P. Cook ◽  
M. L. Parmelee ◽  
...  
Keyword(s):  
Blood Group ◽  
Potassium Transport ◽  
Red Cells ◽  
Sheep Red Cells ◽  
Stimulation Of

scholarly journals THE EFFECTS OF ANTIGENS AND OF PHYTOHEMAGGLUTININ ON RABBIT SPLEEN CELL SUSPENSIONS

1966 ◽  
Vol 124 (4) ◽  
pp. 621-634 ◽  
Author(s):  
G. Harris ◽  
R. J. Littleton
Keyword(s):  
Dna Synthesis ◽  
Spleen Cell ◽  
Cell Suspensions ◽  
Red Cells ◽  
Specific Response ◽  
Spleen Cells ◽  
Sheep Red Cells ◽  
Stimulation Of

Phytohemagglutinin (PHA) stimulated the rate of DNA synthesis in rabbit spleen cell suspensions. Unlike antigens, previous immunization to PHA was not necessary and the specific response could not be transferred by macrophages, although lymphocytes primed by incubation in PHA were able to stimulate other spleen cells not directly exposed to PHA. When rabbits were stimulated by in vivo immunization with antigens, spleen cells proliferating in response to antigen were stimulated to divide by in vitro contact with PHA. Using the technique of specific hemolytic plaque formation by individual cells synthesizing γM-antibody to sheep red cells (plaque-forming cells), no evidence was obtained that stimulation of cell division by PHA resulted in specific antibody formation, although the presence of antigen resulted both in stimulation of cell proliferation and the production of plaque-forming cells. The presence of both sheep red cells and PHA in the medium of the same cell suspensions did not enhance the production of plaque-forming cells although there was a summative effect on DNA synthesis.

High potassium type red cells in cattle

1970 ◽  
Vol 74 (3) ◽  
pp. 595-596 ◽  
Author(s):  
J. C. Ellory ◽  
Elizabeth M. Tucker
Keyword(s):  
Potassium Transport ◽  
Potassium Uptake ◽  
Red Cells ◽  
Red Cell ◽  
High Potassium ◽  
Sheep Red Cells ◽  
Antigen System ◽  
Jersey Cows ◽  
Two Populations

SUMMARYThe distribution of red cell potassium concentrations in ninety–one Jersey cows was examined, and shown to be consistent with two populations, a high (HK) and low (LK) type. In tracer potassium uptake experiments the HK type cells were shown to have a higher active K+ uptake, and lower passive K+ leak than LK type cells. Tests with the sheep anti–L antibody showed that the L–antigen system, which controls active potassium transport in sheep red cells does not appear to act in cattle red cells.

The number and specificity of L antigen sites on low potassium type sheep red cells

1976 ◽  
Vol 194 (1115) ◽  
pp. 271-277 ◽  
Keyword(s):  
Electron Microscopy ◽  
Potassium Transport ◽  
Red Cells ◽  
Sheep Red Cells ◽  
Transport Studies ◽  
Low Potassium

Pig anti-sheep IgG conjugated, to haemocyanin was used as a marker to visualize by electron microscopy the L and. M antigen sites on sheep red cells. The number of sites seen correlated well with the results of serological and potassium transport studies and supported the concept that there are qualitative as well as quantitative differences in the expression of L antigen on the membrane of LK type cells. A tentative estimate of 340 L p sites on homozygous LK and 120 on heterozygous LK cells was made.

Depressed blood group antigens on red cells from a Mexican donor

Transfusion ◽  
1983 ◽  
Vol 23 (1) ◽  
pp. 65-66 ◽  
Author(s):  
V Biro ◽  
G Garratty ◽  
CL Johnson ◽  
WL Marsh
Keyword(s):  
Blood Group ◽  
Red Cells ◽  
Blood Group Antigens
Sign in / Sign up

Export Citation Format

Share Document