Gating properties of channels formed by colicin Ia in planar lipid bilayer membranes

1988 ◽  
Vol 105 (2) ◽  
pp. 143-153 ◽  
Author(s):  
Romildo A. Nogueira ◽  
A. Varanda
Keyword(s):  
Lipid Bilayer ◽  
Planar Lipid Bilayer ◽  
Lipid Bilayer Membranes ◽  
Bilayer Membranes ◽  
Colicin Ia

Formation of asymmetrical planar lipid bilayer membranes from characterized monolayers

1983 ◽  
Vol 7 (4) ◽  
pp. 299-310 ◽  
Author(s):  
Pierre Tancrède ◽  
Paul Paquin ◽  
André Houle ◽  
Roger M. Leblanc
Keyword(s):  
Lipid Bilayer ◽  
Planar Lipid Bilayer ◽  
Lipid Bilayer Membranes ◽  
Bilayer Membranes

scholarly journals Reconstitution of bacterial outer membrane TonB-dependent transporters in planar lipid bilayer membranes

2009 ◽  
Vol 106 (51) ◽  
pp. 21990-21995 ◽  
Author(s):  
E. Udho ◽  
K. S. Jakes ◽  
S. K. Buchanan ◽  
K. J. James ◽  
X. Jiang ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Lipid Bilayer ◽  
Planar Lipid Bilayer ◽  
Lipid Bilayer Membranes ◽  
Bilayer Membranes ◽  
Bacterial Outer Membrane

Single sodium channels from rat brain incorporated into planar lipid bilayer membranes

Nature ◽  
1983 ◽  
Vol 303 (5913) ◽  
pp. 172-175 ◽  
Author(s):  
Bruce K. Krueger ◽  
Jennings F. Worley ◽  
Robert J. French
Keyword(s):  
Rat Brain ◽  
Lipid Bilayer ◽  
Sodium Channels ◽  
Planar Lipid Bilayer ◽  
Lipid Bilayer Membranes ◽  
Bilayer Membranes ◽  
Single Sodium Channels

A quartz cell for studying planar lipid bilayer membranes

1982 ◽  
Vol 688 (3) ◽  
pp. 808-810 ◽  
Author(s):  
Joaquim Procopio ◽  
Wamberto A. Varanda ◽  
JoséA. Fornes
Keyword(s):  
Lipid Bilayer ◽  
Planar Lipid Bilayer ◽  
Lipid Bilayer Membranes ◽  
Bilayer Membranes

scholarly journals El Tor hemolysin of Vibrio cholerae O1 forms channels in planar lipid bilayer membranes

2006 ◽  
Vol 150 (2) ◽  
pp. 249-254 ◽  
Author(s):  
Hajime Ikigai ◽  
Toshihisa Ono ◽  
Masashi Iwata ◽  
Taiji Nakae ◽  
Tadakatsu Shimamura
Keyword(s):  
Vibrio Cholerae ◽  
Lipid Bilayer ◽  
Planar Lipid Bilayer ◽  
Lipid Bilayer Membranes ◽  
Bilayer Membranes ◽  
Vibrio Cholerae O1 ◽  
El Tor

scholarly journals Oligomeric Structure of Colicin Ia Channel in Lipid Bilayer Membranes

2009 ◽  
Vol 284 (24) ◽  
pp. 16126-16134 ◽  
Author(s):  
Sarah L. Greig ◽  
Mazdak Radjainia ◽  
Alok K. Mitra
Keyword(s):  
Lipid Bilayer ◽  
Large Scale ◽  
Three Dimensional ◽  
Velocity Sedimentation ◽  
Dimensional Structure ◽  
Lipid Bilayer Membranes ◽  
Outer Membrane Receptor ◽  
Bilayer Membranes ◽  
Three Dimensional Structure ◽  
Colicin Ia

Colicin Ia is a soluble, harpoon-shaped bacteriocin which translocates across the periplasmic space of sensitive Escherichia coli cell by parasitizing an outer membrane receptor and forms voltage-gated ion channels in the inner membrane. This process leads to cell death, which has been thought to be caused by a single colicin Ia molecule. To directly visualize the three-dimensional structure of the channel, we generated two-dimensional crystals of colicin Ia inserted in lipid-bilayer membranes and determined a ∼17 three-dimensional model by electron crystallography. Supported by velocity sedimentation, chemical cross-linking and single-particle image analysis, the three-dimensional structure is a crown-shaped oligomer enclosing a ∼35 Å-wide extrabilayer vestibule. Our study suggests that lipid insertion instigates a global conformational change in colicin Ia and that more than one molecule participates in the channel architecture with the vestibule, possibly facilitating the known large scale peptide translocation upon channel opening.

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