Short-term organ culture of the retinal pigment epithelium in microtitration plates: Ultrastructural studies

1986 ◽  
Vol 224 (5) ◽  
pp. 401-406 ◽  
Author(s):  
N. M. McKechnie ◽  
W. A. Keegan ◽  
C. A. Converse ◽  
W. S. Foulds
Keyword(s):  
Retinal Pigment Epithelium ◽  
Organ Culture ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Short Term ◽  
Ultrastructural Studies

Triple Immune Suppression Increases Short-Term Survival of Porcine Fetal Retinal Pigment Epithelium Xenografts

2003 ◽  
Vol 44 (9) ◽  
pp. 4044 ◽  
Author(s):  
Lucian V. Del Priore ◽  
Osamu Ishida ◽  
Eric W. Johnson ◽  
Yaohua Sheng ◽  
Douglas B. Jacoby ◽  
...  
Keyword(s):  
Retinal Pigment Epithelium ◽  
Immune Suppression ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Short Term ◽  
Term Survival ◽  
Short Term Survival

Short-term study of retinal pigment epithelium sheet transplants onto Bruch's membrane

2004 ◽  
Vol 78 (1) ◽  
pp. 53-65 ◽  
Author(s):  
Hao Wang ◽  
Fumihiko Yagi ◽  
Noounanong Cheewatrakoolpong ◽  
Ilene K. Sugino ◽  
Marco A. Zarbin
Keyword(s):  
Retinal Pigment Epithelium ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Bruch's Membrane ◽  
Bruch’S Membrane ◽  
Short Term ◽  
Term Study ◽  
Short Term Study

Chick embryonic pigmented retina is one of the group of epithelioid tissues that lack cytokeratins and desmosomes and have intermediate filaments composed of vimentin

1984 ◽  
Vol 71 (1) ◽  
pp. 61-74
Author(s):  
R.J. Docherty ◽  
J.G. Edwards ◽  
D.R. Garrod ◽  
D.L. Mattey
Keyword(s):  
Retinal Pigment Epithelium ◽  
Intermediate Filaments ◽  
Vascular Endothelium ◽  
Sodium Dodecyl Sulphate ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Sodium Dodecyl ◽  
Major Protein ◽  
Ultrastructural Studies ◽  
Chick Embryonic Tissue

Using sodium dodecyl sulphate/polyacrylamide gels to analyse detergent-insoluble residues, and indirect immunofluorescence, we have found that the major protein of intermediate filaments in cultures and freshly explanted fragments of chick embryonic retinal pigment epithelium (RPE) is vimentin. Moreover, these cells also fail to stain with antibodies against cytokeratins and most components of true desmosomes (maculae adhaerentes). Staining with anti-vinculin antibody suggests that the principal intercellular junction is the zonula adherens. Thus although RPE is an epithelium according to all other criteria, it belongs to a group of tissues (including vascular endothelium, iris and lens-forming epithelium) that have intermediate filaments composed of vimentin and possess neither cytokeratins nor desmosomes. That a tissue can be fully epithelial by other criteria, whilst lacking these components, is in agreement with other work, which has shown a lack of effect of micro-injection of antibodies to cytokeratin, and of the suppression of desmosome formation, on epithelial organization in culture. Although our observations were made solely on chick embryonic tissue, we suggest that published ultrastructural studies are consistent with the possibility that RPE of other species, including human, may lack true desmosomes.

Morphology of Pig Retinal Pigment Epithelium Maintained in Organ Culture

1988 ◽  
Vol 106 (9) ◽  
pp. 1286-1290 ◽  
Author(s):  
L. V. Del Priore ◽  
B. M. Glaser ◽  
H. A. Quigley ◽  
M. E. Dorman ◽  
W. R. Green
Keyword(s):  
Retinal Pigment Epithelium ◽  
Organ Culture ◽  
Pigment Epithelium ◽  
Retinal Pigment
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