Mitochondrial genome size variation and restriction fragment length polymorphisms in threePhaseolus species

1991 ◽  
Vol 82 (3) ◽  
pp. 321-328 ◽  
Author(s):  
M. M. Khairallah ◽  
M. W. Adams ◽  
B. B. Sears
Keyword(s):  
Mitochondrial Genome ◽  
Genome Size ◽  
Restriction Fragment ◽  
Fragment Length ◽  
Size Variation ◽  
Restriction Fragment Length Polymorphisms ◽  
Genome Size Variation ◽  
Length Polymorphisms ◽  
Restriction Fragment Length

scholarly journals Comparison of Restriction Fragment Length Polymorphisms in Chloroplast DNA of Five Leafy Spurge (Euphorbiaspp.) Accessions

Weed Science ◽  
1992 ◽  
Vol 40 (1) ◽  
pp. 63-67 ◽  
Author(s):  
Scott J. Nissen ◽  
Robert A. Masters ◽  
Donald J. Lee ◽  
Martha L. Rowe
Keyword(s):  
Chloroplast Dna ◽  
Chloroplast Genome ◽  
Genome Size ◽  
Restriction Fragment ◽  
Fragment Length ◽  
Mung Bean ◽  
Leafy Spurge ◽  
Restriction Fragment Length Polymorphisms ◽  
Length Polymorphisms ◽  
Restriction Fragment Length

Chloroplast DNA (cpDNA) restriction fragment length polymorphisms (RFLPs) were analyzed to assess genetic variation and relatedness among selections of North American and Eurasian leafy spurge. Leafy spurge accessions from Nebraska, Montana, Russia, Italy, and Austria were evaluated. Total DNA was extracted from young leaves and digested with the restriction endonuclease,EcoRI. CpDNA fragment patterns were determined by Southern blot analysis using mung bean cpDNA probes. Colinearity between the mung bean and leafy spurge chloroplast genomes was indicated by the observation that common fragments were hybridized by adjacent probes. Minimum estimates of chloroplast genome size for the five leafy spurge accessions, which ranged in kilobase size from 130 to 132, were within the size range of most terrestrial plants. Structural collinearity and reasonable estimates of chloroplast genome size provided evidence that the mung bean cpDNA library was suitable for characterizing leafy spurge cpDNA. Seven of the 13 mung bean probes hybridized to polymorphic leafy spurge cpDNA fragments. Based on number of polymorphisms unique to each Eurasian accession, the Austrian accession appeared to be most divergent followed by the Italian and Russian. The North American accessions seem to be most closely related to each other and to the Russian leafy spurge accession.

scholarly journals A genetic linkage map for the domesticated silkworm, Bombyx mori, based on restriction fragment length polymorphisms

1995 ◽  
Vol 66 (2) ◽  
pp. 109-126 ◽  
Author(s):  
Jinrui Shi ◽  
David G. Heckel ◽  
Marian R. Goldsmith
Keyword(s):  
Linkage Map ◽  
Bombyx Mori ◽  
Restriction Fragment ◽  
Fragment Length ◽  
Restriction Fragment Length Polymorphisms ◽  
Genetic Maps ◽  
Crossing Over ◽  
Linkage Groups ◽  
Length Polymorphisms ◽  
Restriction Fragment Length

SummaryWe present data for the initial construction of a molecular linkage map for the domesticated silkworm, Bombyx mori, based on 52 progeny from an F2 cross from a pair mating of inbred strains p50 and C108, using restriction fragment length polymorphisms (RFLPs). The map contains 15 characterized single copy sequences, 36 anonymous sequences derived from a follicular cDNA library, and 10 loci corresponding to a low copy number retrotransposon, mag. The 15 linkage groups and 8 ungrouped loci account for 23 of the 28 chromosomes and span a total recombination length of 413 cM; 10 linkage groups were correlated with established classic genetic maps. Scoring data from Southern blots were analysed using two Pascal programs written specifically to analyse linkage data in Lepidoptera, where females are the heterogametic sex and have achiasmatic meiosis (no crossing-over). These first examine evidence for linkage by calculating the maximum lod score under the hypothesis that the two loci are linked over the likelihood under the hypothesis that the two loci assort independently, and then determine multilocus linkage maps for groups of putatively syntenic loci by calculating the maximum likelihood estimate of the recombination fractions and the log likelihood using the EM algorithm for a specified order of loci along the chromosome. In addition, the possibility of spurious linkage was exhaustively tested by searching for genotypes forbidden by the absence of crossing-over in one sex.

Analysis of Restriction Fragment Length Polymorphisms in Rheumatic Diseases

1987 ◽  
Vol 13 (2) ◽  
pp. 353-367
Author(s):  
D. Olga McDaniel ◽  
Bruce O. Barger ◽  
John D. Reveille ◽  
Graciela S. Alarcón ◽  
William J. Koopman ◽  
...  
Keyword(s):  
Rheumatic Diseases ◽  
Restriction Fragment ◽  
Fragment Length ◽  
Restriction Fragment Length Polymorphisms ◽  
Length Polymorphisms ◽  
Restriction Fragment Length
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