Rat bone marrow erythroid cell fractionation by counter current distribution in non-charge-sensitive two-phase systems

1992 ◽  
Vol 12 (2) ◽  
pp. 77-85 ◽  
Author(s):  
A. I. Garcia-Pérez ◽  
P. Sancho ◽  
J. Mendieta ◽  
J. Luque
Keyword(s):  
Bone Marrow ◽  
Distribution Coefficient ◽  
Current Distribution ◽  
Erythroid Cell ◽  
Cell Fractionation ◽  
Two Phase ◽  
Phase Systems ◽  
Counter Current ◽  
Counter Current Distribution ◽  
Rat Bone

Counter-current distribution in non charge-sensitive aqueous poly(ethylene glycol)-dextran two phase systems allows the fractionation of rat bone marrow cells into two broad cell subpopulations with different distribution coefficients in a relatively short time. Morphological identification and enzymatic studies suggest that erythroid cells are mainly present in the subpopulation with the higher distribution coefficient. The distribution coefficient and, therefore, surface hydrophobicity of these cells, apparently increase in parallel with an increase in their degree of differentiation and maturation.

Surface properties of crosslinked erythrocytes as studied by counter-current distribution in aqueous polymer two-phase systems

1995 ◽  
Vol 664 (1) ◽  
pp. 137-144 ◽  
Author(s):  
Luisa Lucas ◽  
Ana-Isabel García-Pérez ◽  
Pilar Jimeno ◽  
M. Teresa Pérez ◽  
Montserrat Pinilla ◽  
...  
Keyword(s):  
Surface Properties ◽  
Current Distribution ◽  
Two Phase ◽  
Aqueous Polymer ◽  
Phase Systems ◽  
Counter Current ◽  
Counter Current Distribution

Heterogeneity of hypotonically loaded rat erythrocyte populations as detected by counter-current distribution in aqueous polymer two-phase systems

1996 ◽  
Vol 677 (1) ◽  
pp. 45-51 ◽  
Author(s):  
M. Teresa Pe´rez ◽  
F. Javier Alvarez ◽  
Ana-Isabel Garci´a-Pe´rez ◽  
Luisa Lucas ◽  
M. Cristina Tejedor ◽  
...  
Keyword(s):  
Current Distribution ◽  
Two Phase ◽  
Aqueous Polymer ◽  
Phase Systems ◽  
Counter Current ◽  
Counter Current Distribution

Transferrin binding capacity as a marker of differentiation and maturation of rat erythroid cells fractionated by counter current distribution in aqueous polymer two-phase systems

1994 ◽  
Vol 14 (3) ◽  
pp. 119-130
Author(s):  
P. Sancho ◽  
J. Mendieta ◽  
A. I. García-Pérez ◽  
J. Luque
Keyword(s):  
Bone Marrow ◽  
Binding Capacity ◽  
Marrow Cell ◽  
Erythroid Cells ◽  
Two Phase ◽  
Cell Ratio ◽  
Phase Systems ◽  
Cell Fractions ◽  
Counter Current ◽  
Counter Current Distribution

Rat bone marrow cell populations, containing different proportions of erythroid cells, have been fractionated by counter-current distribution in the non-charge-sensitive dextran/polyethyleneglycol two-phase systems on the basis of hydrophobic cell surface properties. Cell fractions with a low distribution coefficient, which contain non-erythroid cells and early erythoblasts, showed a low transferrin binding capacity and a low haemoglobin/cell ratio whereas cell fractions with a high distribution coefficient, which contain intermediate-late erythroblasts and mature red cells, showed an elevated transferrin binding capacity and the highest haemoglobin/cell ratio. These results support transferrin binding capacity as a good marker parameter for the erythroid bone marrow cell differentiation and maturation processes.

Biochemical characterization of human erythrocytes fractionated by counter-current distribution in aqueous polymer two-phase systems

1994 ◽  
Vol 668 (1) ◽  
pp. 165-171 ◽  
Author(s):  
Montserrat Pinilla ◽  
Jesus de la Fuente ◽  
Ana I. García-Pérez ◽  
Pilar Jimeno ◽  
Pilar Sancho ◽  
...  
Keyword(s):  
Current Distribution ◽  
Biochemical Characterization ◽  
Human Erythrocytes ◽  
Two Phase ◽  
Aqueous Polymer ◽  
Phase Systems ◽  
Counter Current ◽  
Counter Current Distribution
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