Fractionation of wheat proteins by counter-current distribution using aqueous two-phase systems containing propionic acid

1996 ◽  
Vol 680 (1-2) ◽  
pp. 71-80 ◽  
Author(s):  
Helle Truust ◽  
Göte Johansson
Keyword(s):  
Propionic Acid ◽  
Current Distribution ◽  
Wheat Proteins ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Counter Current ◽  
Counter Current Distribution

Heterogeneity of hypotonically loaded rat erythrocyte populations as detected by counter-current distribution in aqueous polymer two-phase systems

1996 ◽  
Vol 677 (1) ◽  
pp. 45-51 ◽  
Author(s):  
M. Teresa Pe´rez ◽  
F. Javier Alvarez ◽  
Ana-Isabel Garci´a-Pe´rez ◽  
Luisa Lucas ◽  
M. Cristina Tejedor ◽  
...  
Keyword(s):  
Current Distribution ◽  
Two Phase ◽  
Aqueous Polymer ◽  
Phase Systems ◽  
Counter Current ◽  
Counter Current Distribution

Biochemical characterization of human erythrocytes fractionated by counter-current distribution in aqueous polymer two-phase systems

1994 ◽  
Vol 668 (1) ◽  
pp. 165-171 ◽  
Author(s):  
Montserrat Pinilla ◽  
Jesus de la Fuente ◽  
Ana I. García-Pérez ◽  
Pilar Jimeno ◽  
Pilar Sancho ◽  
...  
Keyword(s):  
Current Distribution ◽  
Biochemical Characterization ◽  
Human Erythrocytes ◽  
Two Phase ◽  
Aqueous Polymer ◽  
Phase Systems ◽  
Counter Current ◽  
Counter Current Distribution

Rat bone marrow erythroid cell fractionation by counter current distribution in non-charge-sensitive two-phase systems

1992 ◽  
Vol 12 (2) ◽  
pp. 77-85 ◽  
Author(s):  
A. I. Garcia-Pérez ◽  
P. Sancho ◽  
J. Mendieta ◽  
J. Luque
Keyword(s):  
Bone Marrow ◽  
Distribution Coefficient ◽  
Current Distribution ◽  
Erythroid Cell ◽  
Cell Fractionation ◽  
Two Phase ◽  
Phase Systems ◽  
Counter Current ◽  
Counter Current Distribution ◽  
Rat Bone

Counter-current distribution in non charge-sensitive aqueous poly(ethylene glycol)-dextran two phase systems allows the fractionation of rat bone marrow cells into two broad cell subpopulations with different distribution coefficients in a relatively short time. Morphological identification and enzymatic studies suggest that erythroid cells are mainly present in the subpopulation with the higher distribution coefficient. The distribution coefficient and, therefore, surface hydrophobicity of these cells, apparently increase in parallel with an increase in their degree of differentiation and maturation.

scholarly journals Changes in glycolytic enzyme activities in aging erythrocytes fractionated by counter-current distribution in aqueous polymer two-phase systems

1991 ◽  
Vol 279 (1) ◽  
pp. 237-243 ◽  
Author(s):  
P Jimeno ◽  
A I Garcia-Perez ◽  
J Luque ◽  
M Pinilla
Keyword(s):  
Pyruvate Kinase ◽  
Current Distribution ◽  
Phosphoglycerate Kinase ◽  
Rat Erythrocytes ◽  
Two Phase ◽  
Phase Systems ◽  
Cell Fractions ◽  
Counter Current ◽  
Counter Current Distribution ◽  
Bisphosphoglycerate Mutase

Human and rat erythrocytes were fractionated by counter-current distribution in charge-sensitive dextran/poly(ethylene glycol) two-phase systems. The specific activities of the key glycolytic enzymes (hexokinase, phosphofructokinase and pyruvate kinase) declined along the distribution profiles, although the relative positions of the activity profiles were reversed in the two species. These enzymes maintained their normal response to specific regulatory effectors in all cell fractions. No variations were observed for phosphoglycerate kinase and bisphosphoglycerate mutase activities. Some correlations between enzyme activities (pyruvate kinase/hexokinase, pyruvate kinase/phosphofructokinase, pyruvate kinase/pyruvate kinase plus phosphoglycerate kinase, pyruvate kinase/bisphosphoglycerate mutase and phosphoglycerate kinase/bisphosphoglycerate mutase ratios) were studied in whole erythrocyte populations as well as in cell fractions. These results strongly support the fractionation of human erythrocytes according to cell age, as occurs with rat erythrocytes.

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