Chenopodium quinoa strains suitable for quantitative assay of potato virus S

1980 ◽  
Vol 23 (1) ◽  
pp. 49-55 ◽  
Author(s):  
T. Matsumoto ◽  
C. Hiruki
Keyword(s):  
Potato Virus ◽  
Chenopodium Quinoa ◽  
Quantitative Assay ◽  
Potato Virus S

scholarly journals Strain Characterization of Potato virus S Isolates from Tasmania, Australia

Plant Disease ◽  
2012 ◽  
Vol 96 (6) ◽  
pp. 813-819 ◽  
Author(s):  
Susan J. Lambert ◽  
Jason B. Scott ◽  
Sarah J. Pethybridge ◽  
Frank S. Hay
Keyword(s):  
Potato Virus ◽  
Chenopodium Quinoa ◽  
Protein Sequencing ◽  
Potato Seed ◽  
Serological Detection ◽  
Potato Virus S ◽  
Strain Characterization ◽  
Geographical Regions ◽  
Local Lesions

Potato virus S (PVS) is prevalent within potato (Solanum tuberosum) production worldwide. Traditionally, PVS has been split into two strains, Ordinary (PVSO) and Andean (PVSA), based on reaction in herbaceous indicator species such as Chenopodium quinoa. However, recent research has identified further strain designations, such as PVSO-CS (Ordinary and Chenopodium systemic). Forty-four isolates of PVS were collected from potato seed lines in different geographical regions within Tasmania, Australia. Isolates were initially characterized by reactions in C. quinoa. Nineteen isolates were characterized as PVSO, based on the development of local lesions and serological detection in inoculated leaves only. Three isolates were identified as PVSA-like, based on local lesion development in inoculated leaves, mild mottling or chlorotic spots on noninoculated leaves, and serological detection in both inoculated and noninoculated leaves. Thirteen isolates produced no symptoms, and were detected serologically in inoculated leaves only (PVSO-like). Four isolates produced no symptoms but were detected serologically in both inoculated and noninoculated leaves (PVSA-like). Five isolates produced symptoms in inoculated leaves only but were detected serologically in both inoculated and noninoculated leaves (also PVSA-like). The ability of isolates to infect tomato has also been used as a criterion to assist in PVS strain differentiation. A subsample of isolates (n = 16) was unable to infect tomato ‘Grosse Lisse’. Seventeen isolates representative of these groupings based on reactions in C. quinoa were also characterized by coat-protein sequencing. Phylogenetic comparisons suggested that all isolates were PVSO rather than PVSA. Therefore, whereas some of these PVS isolates were systemic in C. quinoa, findings from this study suggest that they were not PVSA, and that only PVSO and PVSO-CS isolates are present in Tasmania. The implications of this finding for disease management are discussed.

Intracellular location of potato virus S in leaf tissue of Chenopodium quinoa

1973 ◽  
Vol 51 (9) ◽  
pp. 1699-1702 ◽  
Author(s):  
C. Hlruki ◽  
P. Shukla
Keyword(s):  
Potato Virus ◽  
Serological Test ◽  
Chenopodium Quinoa ◽  
Leaf Tissue ◽  
Potato Virus S ◽  
Thin Sections ◽  
Intracellular Location ◽  
Cellular Inclusions ◽  
Close Proximity ◽  
Local Lesions

The presence of potato virus S (PVS) in the local lesions of Chenopodium quinoa was shown by bioassay, a serological test, and electron microscopy of negatively stained preparations of the infected tissues. Amorphous aggregates of particles believed to be PVS were found with the electron microscope in ultra-thin sections of both necrotic and nearby chlorotic cells of lesions sampled 8 days after inoculation of C. quinoa. The virus masses were confined to the cytoplasm and found in close proximity to the nucleus, chloroplasts, and tonoplasts. No other cellular inclusions were found in these cells.

Detection of potato virus M and potato virus S on test plants

1976 ◽  
Vol 19 (2) ◽  
pp. 131-139 ◽  
Author(s):  
A. Kowalska ◽  
M. Waś
Keyword(s):  
Potato Virus ◽  
Potato Virus S ◽  
Potato Virus M ◽  
Test Plants

Spatiotemporal Spread of Potato virus S and Potato virus X in Seed Potato in Tasmania, Australia

2007 ◽  
Vol 8 (1) ◽  
pp. 70 ◽  
Author(s):  
Susan J. Lambert ◽  
Frank S. Hay ◽  
Sarah J. Pethybridge ◽  
Calum R. Wilson
Keyword(s):  
Seed Potato ◽  
Potato Virus ◽  
Temporal Distribution ◽  
Potato Virus X ◽  
Russet Burbank ◽  
Mechanical Transmission ◽  
Spatial And Temporal Distribution ◽  
Plant Spacing ◽  
Potato Virus S ◽  
The Mean

The spatial and temporal distribution of Potato virus S (PVS) and Potato virus X (PVX) was studied in two trials within each of four commercial fields of seed potato var. Russet Burbank in Tasmania, Australia. In the first trial (plots) 20 leaflets were collected from each of 49 plots (each approximately 8 m wide by 10 m long), with plots arranged in a 7-×-7 lattice. In the second trial (transects), leaflets were collected at 1-m intervals along seven adjacent, 50-m long rows. The mean incidence of PVS increased during the season by 5.2% in one of four plot trials and 25.5% in one of four transect trials. The mean incidence of PVX increased during the season by 10.1%, in one of two transect trials. Spatial Analysis by Distance IndicEs and ordinary runs analysis detected aggregation of PVS infected plants early in the season in one and two fields respectively, suggesting transmission during seed-cutting or during planting. An increase in PVS incidence mid- to late season in one field was associated with aggregation of PVS along, but not across rows, which may be related to the closer plant spacing within rows and hence increased potential for mechanical transmission along rows. Results suggested limited spread of PVS and PVX occurred within crops during the season. Accepted for publication 9 April 2007. Published 26 July 2007.

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