Fish mapping of a translocation breakpoint at 6q21 (or q22) in a patient with heterotaxia

1997 ◽  
Vol 42 (4) ◽  
pp. 525-532 ◽  
Author(s):  
Rumiko Kato ◽  
Naomichi Matsumoto ◽  
Masahiro Fujimoto ◽  
Motoi Nakano ◽  
Yusuke Nakamura ◽  
...  
Keyword(s):  
Translocation Breakpoint ◽  
Fish Mapping

An extended river buffalo (Bubalus bubalis, 2n = 50) cytogenetic map: assignment of 68 autosomal loci by FISH-mapping and R-banding and comparison with human chromosomes

2008 ◽  
Vol 16 (6) ◽  
pp. 827-837 ◽  
Author(s):  
G. P. Di Meo ◽  
A. Perucatti ◽  
S. Floriot ◽  
H. Hayes ◽  
L. Schibler ◽  
...  
Keyword(s):  
Bubalus Bubalis ◽  
River Buffalo ◽  
Human Chromosomes ◽  
Cytogenetic Map ◽  
Fish Mapping

Translocation breakpoint in Aarskog syndrome maps to Xp11.21 between ALAS2 and DXS323

1993 ◽  
Vol 2 (10) ◽  
pp. 1717-1718 ◽  
Author(s):  
Thomas W. Glover ◽  
Vera Verga ◽  
Jill Rafael ◽  
Christine Barcroft ◽  
Jerome L. Gorski ◽  
...  
Keyword(s):  
Translocation Breakpoint ◽  
Aarskog Syndrome

Cloning and characterization of the human t(3;6)(p14;p11) translocation breakpoint associated with hematologic malignancies

1993 ◽  
Vol 71 (1) ◽  
pp. 15-21 ◽  
Author(s):  
Scott E. Smith ◽  
Anthony Joseph ◽  
Scott Nadeau ◽  
Viji Shridhar ◽  
Robert Gemmill ◽  
...  
Keyword(s):  
Hematologic Malignancies ◽  
Translocation Breakpoint

CD3G is within 200 kb of the leukemic t(4;II) translocation breakpoint

1991 ◽  
Vol 3 (1) ◽  
pp. 44-47 ◽  
Author(s):  
Soma Das ◽  
Finbarr E. Cotter ◽  
Barbara Gibbons ◽  
Susheela Dhut ◽  
Bryan D. Young
Keyword(s):  
Translocation Breakpoint

High-Resolution Pachytene Chromosome Mapping of Bacterial Artificial Chromosomes Anchored by Genetic Markers Reveals the Centromere Location and the Distribution of Genetic Recombination Along Chromosome 10 of Rice

Genetics ◽  
2001 ◽  
Vol 157 (4) ◽  
pp. 1749-1757 ◽  
Author(s):  
Zhukuan Cheng ◽  
Gernot G Presting ◽  
C Robin Buell ◽  
Rod A Wing ◽  
Jiming Jiang
Keyword(s):  
Large Scale ◽  
Genetic Recombination ◽  
Physical Map ◽  
Rice Chromosome ◽  
Resolving Power ◽  
Pachytene Chromosome ◽  
Chromosome 10 ◽  
Bac Clone ◽  
Fish Mapping ◽  
Artificial Chromosomes

AbstractLarge-scale physical mapping has been a major challenge for plant geneticists due to the lack of techniques that are widely affordable and can be applied to different species. Here we present a physical map of rice chromosome 10 developed by fluorescence in situ hybridization (FISH) mapping of bacterial artificial chromosome (BAC) clones on meiotic pachytene chromosomes. This physical map is fully integrated with a genetic linkage map of rice chromosome 10 because each BAC clone is anchored by a genetically mapped restriction fragment length polymorphism marker. The pachytene chromosome-based FISH mapping shows a superior resolving power compared to the somatic metaphase chromosome-based methods. The telomere-centromere orientation of DNA clones separated by 40 kb can be resolved on early pachytene chromosomes. Genetic recombination is generally evenly distributed along rice chromosome 10. However, the highly heterochromatic short arm shows a lower recombination frequency than the largely euchromatic long arm. Suppression of recombination was found in the centromeric region, but the affected region is far smaller than those reported in wheat and barley. Our FISH mapping effort also revealed the precise genetic position of the centromere on chromosome 10.

Characterization of a fusion cDNA (RARA/myl) transcribed from the t(15;17) translocation breakpoint in acute promyelocytic leukemia

1992 ◽  
Vol 12 (2) ◽  
pp. 800-810
Author(s):  
K S Chang ◽  
S A Stass ◽  
D T Chu ◽  
L L Deaven ◽  
J M Trujillo ◽  
...  
Keyword(s):  
Cdna Library ◽  
Acute Promyelocytic Leukemia ◽  
Blot Analysis ◽  
Blot Hybridization ◽  
Promyelocytic Leukemia ◽  
Dna Probe ◽  
Translocation Breakpoint ◽  
Chromosome 15 ◽  
Dot Blot ◽  
Mrna Species

A nonrandom chromosomal translocation breakpoint, t(15;17)(q22;q21), is found in almost all patients with acute promyelocytic leukemia (APL). Most of these breakpoints occur within the second intron of the retinoic acid receptor-alpha (RARA) gene. We screened a cDNA library of APL and have identified and sequenced a cDNA transcribed from the t(15;17) translocation breakpoint. The 5' end of cDNA p1715 consists of 503 bp of the RARA exon II sequence. A 1.76-kb cDNA without homology to any known gene available in GenBank was found truncated downstream. This cDNA sequence was assigned to chromosome 15 by dot blot hybridization of the flow cytometry-sorted chromosomes. We designate this fusion cDNA RARA/myl, which is different from myl/RARA reported by de The et al. (H. de The, C. Chomienne, M. Lanotte, L. Degos, and A. Dejean, Nature (London) 347:558-561, 1990). This result demonstrates that the two different types of hybrid mRNA can be transcribed from this breakpoint. We screened a non-APL cDNA library and identified a 2.8-kb myl cDNA. This cDNA is able to encode a polypeptide with a molecular weight of 78,450. Alternative splicing of the myl gene which resulted in myl proteins with different C terminals was found. Southern blot analysis of the genomic DNA isolated from 17 APL patients by using the myl DNA probe demonstrated that the myl gene in 12 samples was rearranged. Northern (RNA) blot analysis of RARA gene expression in two APL RNA samples showed abnormal mRNA species of 4.2 and 3.2 kb in one patient and of 4.8 and 3.8 kb in another patient; these were in addition to the normal mRNA species of 3.7 and 2.7-kb. The myl DNA probe detected a 2.6-kb abnormal mRNA in addition to the normal mRNA species of 3.2, 4.2, and 5.5 kb. Using the polymerase chain reaction, we demonstrated that both RARA/myl and myl/RARA were coexpressed in samples from three different APL patients. From this study, we conclude that the t(15;17) translocation breakpoint results in the transcription of two different fusion transcripts which are expected to be translated into fusion proteins.

scholarly journals FISH Mapping of the 5S and 18S-28S rDNA Loci in Different Species of Glycine

2001 ◽  
Vol 92 (3) ◽  
pp. 295-300 ◽  
Author(s):  
P. Krishnan ◽  
V. T. Sapra ◽  
K. M. Soliman ◽  
A. Zipf
Keyword(s):  
28S Rdna ◽  
Fish Mapping ◽  
Rdna Loci
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