Ligninolytic enzyme production and the ability of decolourisation of Poly R-478 in packed-bed bioreactors by Phanerochaete chrysosporium

2000 ◽  
Vol 23 (3) ◽  
pp. 287-293 ◽  
Author(s):  
S. Rodríguez Couto ◽  
I. Rivela ◽  
M. R. Muñoz ◽  
A. Sanromán
2001 ◽  
Vol 37 (5) ◽  
pp. 549-554 ◽  
Author(s):  
Alberto Domı́nguez ◽  
Isabel Rivela ◽  
Susana Rodrı́guez Couto ◽  
Ma Ángeles Sanromán

2003 ◽  
Vol 23 (1) ◽  
pp. 65-74 ◽  
Author(s):  
S. Rodríguez Couto ◽  
R. Rodríguez ◽  
P. P. Gallego ◽  
A. Sanromán

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Caroline Henn ◽  
Diego Alves Monteiro ◽  
Mauricio Boscolo ◽  
Roberto da Silva ◽  
Eleni Gomes

2013 ◽  
Vol 43 ◽  
pp. 146-156 ◽  
Author(s):  
Pratibha Srivastava ◽  
Peter C. Andersen ◽  
James J. Marois ◽  
David L. Wright ◽  
Mrittunjai Srivastava ◽  
...  

Biologia ◽  
2014 ◽  
Vol 69 (11) ◽  
Author(s):  
Denisse González-Ramírez ◽  
Claudia Muro-Urista ◽  
Ainhoa Arana-Cuenca ◽  
Alejandro Téllez-Jurado ◽  
Aldo González-Becerra

AbstractEnzyme production by immobilized Phanerochaete chrysosporium was evaluated in airlift bioreactor and agitated cultures. Free mycelium and immobilized mycelium on alginate beads were tested in the decolourization of 50 and 500 mg/L of Remazol Brilliant Blue R. Dye concentration did not inhibit the fungi development in all tests. In addition, high decolourization percentage of dye was found with free mycelium (99%) in agitated flasks and with immobilized mycelium in airlift (98%). However, decolourization period by immobilized mycelium (120 h) was greater than that by the free mycelium (14 h). Important manganese peroxidase, lignine peroxidase and laccase activities were identified in decolourization process. Manganese peroxidase appeared to be promoted by high dye concentrations during the treatment with immobilized mycelium, but this enzyme was not detected with free mycelium in airlift. Bioreactor prompted also laccase and lignine peroxidase actions in both tests; free mycelium registered a maximum laccase action of 31.569 × 103 U/L in 70 h, whereas immobilized mycelium registered 1.680 × 103 U/L in 170 h, while lignine peroxidase secretion by free P. chrysosporium was higher (1.300 × 103 U/L) than immobilized mycelium (1.250 × 103 U/L). Maximum laccase activity coincided with the maximum percentage of decolourization, however, high peroxidase activity was identified from the start of dye treatment.


2006 ◽  
Vol 58 (3) ◽  
pp. 179-182 ◽  
Author(s):  
Jelena Vukojevic ◽  
Mirjana Stajic ◽  
Sonja Duletic-Lausevic ◽  
Jasmina Simonic

The effect of initial medium pH on biomass, extracellular and intracellular polysaccharide, and ligninolytic enzyme production by Ganoderma lucidum was investigated at different pH values after 7 and 14 days of cultivation. Maximal production of biomass was recorded at pH 4.5 and 5.0; maximal production of extracellular polysaccharides at pH 7.0 and 3.0; and maximal production of intracellular polysaccharides at pH 7.0 and 5.5. Ligninolytic enzymes were not produced at any pH of the medium. Maximal biomass production was obtained on the 11th day of cultivation; maximal extracellular polysaccharide production on the 7th day; and maximal intracellular polysaccharide production on the 6th and 10th day of cultivation. .


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