Laccase Gene LAC1 of Colletotrichum lagenarium Is Not Essential for Melanin Biosynthesis and Pathogenicity

2001 ◽  
Vol 67 (3) ◽  
pp. 182-190 ◽  
Author(s):  
Gento TSUJI ◽  
Junko FUJIKAWA ◽  
Hideaki ISHIDA ◽  
Osamu HORINO ◽  
Yasuyuki KUBO
Keyword(s):  
Laccase Gene ◽  
Colletotrichum Lagenarium ◽  
Melanin Biosynthesis

Relation between pigment intensity and penetrating ability in appressoria of Colletotrichum lagenarium

1987 ◽  
Vol 33 (10) ◽  
pp. 870-873 ◽  
Author(s):  
Yasuyuki Kubo ◽  
Iwao Furusawa ◽  
Jiko Shishiyama
Keyword(s):  
Parent Strain ◽  
Colletotrichum Lagenarium ◽  
Melanin Biosynthesis ◽  
Glass Slides ◽  
Albino Mutant

Mutant 8004 of Colletotrichum lagenarium formed slightly pigmented appressoria. The appressoria germinated laterally on glass slides, but could penetrate nitrocellulose membranes. The slightly pigmented appressoria of the albino mutant 79215 were observed to behave in a similar manner when treated with 100 μM scytalone. The appressorial pigmentation of mutant 8004 was increased by the application of scytalone, and mature appressorial pigmentation, indistinguishable from that of the parent strain 104-T, was observed when appressoria were treated with 100 μM scytalone, a lower concentration than that needed for mature pigmentation of the albino mutant. The sensitivity to tricyclazole of appressorial pigmentation of mutant 8004 was higher than that of the parent strain 104-T. These results suggest that, although melanin biosynthesis is essential for appressorial penetration, slight pigmentation is sufficient.

scholarly journals Inhibitory effect of aflastatin A on melanin biosynthesis by Colletotrichum lagenarium

Microbiology ◽  
2001 ◽  
Vol 147 (9) ◽  
pp. 2623-2628 ◽  
Author(s):  
Susumu Okamoto ◽  
Masaru Sakurada ◽  
Yasuyuki Kubo ◽  
Gento Tsuji ◽  
Isao Fujii ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
Colletotrichum Lagenarium ◽  
Melanin Biosynthesis

scholarly journals Multiple Contributions of Peroxisomal Metabolic Function to Fungal Pathogenicity in Colletotrichum lagenarium

2006 ◽  
Vol 72 (9) ◽  
pp. 6345-6354 ◽  
Author(s):  
Makoto Asakura ◽  
Tetsuro Okuno ◽  
Yoshitaka Takano
Keyword(s):  
Host Plant ◽  
Isocitrate Lyase ◽  
Glyoxylate Cycle ◽  
Acetate Metabolism ◽  
Colletotrichum Lagenarium ◽  
Melanin Biosynthesis ◽  
Fungal Pathogenicity ◽  
Peroxisomal Targeting ◽  
The Glyoxylate Cycle ◽  
Host Invasion

ABSTRACT In Colletotrichum lagenarium, which is the causal agent of cucumber anthracnose, PEX6 is required for peroxisome biogenesis and appressorium-mediated infection. To verify the roles of peroxisome-associated metabolism in fungal pathogenicity, we isolated and functionally characterized ICL1 of C. lagenarium, which encodes isocitrate lyase involved in the glyoxylate cycle in peroxisomes. The icl1 mutants failed to utilize fatty acids and acetate for growth. Although Icl1 has no typical peroxisomal targeting signals, expression analysis of the GFP-Icl1 fusion protein indicated that Icl1 localizes in peroxisomes. These results indicate that the glyoxylate cycle that occurs inside the peroxisome is required for fatty acid and acetate metabolism for growth. Importantly, in contrast with the pex6 mutants that form nonmelanized appressoria, the icl1 mutants formed appressoria that were highly pigmented with melanin, suggesting that the glyoxylate cycle is not essential for melanin biosynthesis in appressoria. However, the icl1 mutants exhibited a severe reduction in virulence. Appressoria of the icl1 mutants failed to develop penetration hyphae in the host plant, suggesting that ICL1 is involved in host invasion. The addition of glucose partially restored virulence of the icl1 mutant. Heat shock treatment of the host plant also enabled the icl1 mutants to develop lesions, implying that the infection defect of the icl1 mutant is associated with plant defense. Together with the requirement of PEX6 for appressorial melanization, our findings suggest that peroxisomal metabolic pathways play functional roles in appressorial melanization and subsequent host invasion steps, and the latter step requires the glyoxylate cycle.

Structural analysis of PKS1, a polyketide synthase gene involved in melanin biosynthesis in Colletotrichum lagenarium

1995 ◽  
Vol 249 (2) ◽  
pp. 162-167 ◽  
Author(s):  
Yoshitaka Takano ◽  
Yasuyuki Kubo ◽  
Kiminori Shimizu ◽  
Kazuyuki Mise ◽  
Tetsuro Okuno ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Polyketide Synthase ◽  
Colletotrichum Lagenarium ◽  
Melanin Biosynthesis ◽  
Polyketide Synthase Gene

scholarly journals Isolation of Abikoviromycin and Dihydroabikoviromycin as Inhibitors of Polyketide Synthase Involved in Melanin Biosynthesis by Colletotrichum lagenarium

2003 ◽  
Vol 56 (9) ◽  
pp. 801-804 ◽  
Author(s):  
HIROKI MARUYAMA ◽  
SUSUMU OKAMOTO ◽  
YASUYUKI KUBO ◽  
GENTO TSUJI ◽  
ISAO FUJII ◽  
...  
Keyword(s):  
Polyketide Synthase ◽  
Colletotrichum Lagenarium ◽  
Melanin Biosynthesis

Evidence for involvement of two naphthol reductases in the first reduction step of melanin biosynthesis pathway of Colletotrichum lagenarium

2003 ◽  
Vol 107 (7) ◽  
pp. 854-860 ◽  
Author(s):  
Gento Tsuji ◽  
Tomomi Sugahara ◽  
Isao Fujii ◽  
Yuichiro Mori ◽  
Yutaka Ebizuka ◽  
...  
Keyword(s):  
Biosynthesis Pathway ◽  
Reduction Step ◽  
Colletotrichum Lagenarium ◽  
Melanin Biosynthesis
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