Discovery of novel MHC-class I alleles and haplotypes in Filipino cynomolgus macaques (Macaca fascicularis) by pyrosequencing and Sanger sequencing

2015 ◽  
Vol 67 (10) ◽  
pp. 563-578 ◽  
Author(s):  
Takashi Shiina ◽  
Yukiho Yamada ◽  
Alice Aarnink ◽  
Shingo Suzuki ◽  
Anri Masuya ◽  
...  
Keyword(s):  
Mhc Class I ◽  
Sanger Sequencing ◽  
Macaca Fascicularis ◽  
Class I ◽  
Cynomolgus Macaques

scholarly journals MHC class I allele diversity in cynomolgus macaques of Vietnamese origin

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7941
Author(s):  
Shuting Huang ◽  
Xia Huang ◽  
Shuang Li ◽  
Mingjun Zhu ◽  
Min Zhuo
Keyword(s):  
Mhc Class I ◽  
Binding Sites ◽  
Macaca Fascicularis ◽  
Peptide Binding ◽  
Cynomolgus Macaque ◽  
Class I ◽  
Histocompatibility Complex ◽  
Cynomolgus Macaques ◽  
Mhc Alleles ◽  
Potential Recombination Event

Cynomolgus macaques (Macaca fascicularis, Mafa) have been used as important experimental animal models for carrying out biomedical researches. The results of biomedical experiments strongly depend on the immunogenetic background of animals, especially on the diversity of major histocompatibility complex (MHC) alleles. However, there is much less information available on the polymorphism of MHC class I genes in cynomolgus macaques, than is currently available for humans. In this study, we have identified 40 Mafa-A and 60 Mafa-B exons 2 and 3 sequences from 30 unrelated cynomolgus macaques of Vietnamese origin. Among these alleles, 28 are novel. As for the remaining 72 known alleles, 15 alleles are shared with other cynomolgus macaque populations and 32 are identical to alleles previously reported in other macaque species. A potential recombination event was observed between Mafa-A1*091:02 and Mafa-A1*057:01. In addition, the Mafa-A1 genes were found to be more diverse than human HLA-A and the functional residues for peptide binding sites (PBS) or TCR binding sites (TBS) in Mafa-A1 have greater variability than that for non-PBS or non-TBS regions. Overall, this study provides important information on the diversity of Mafa-A and Mafa-B alleles from Vietnamese origin, which may help researchers to choose the most appropriate animals for their studies.

scholarly journals Characterization of 100 extended major histocompatibility complex haplotypes in Indonesian cynomolgus macaques

2019 ◽  
Author(s):  
Cecilia G. Shortreed ◽  
Roger W. Wiseman ◽  
Julie A. Karl ◽  
Hailey E. Bussan ◽  
David A. Baker ◽  
...  
Keyword(s):  
Major Histocompatibility Complex ◽  
Mhc Class I ◽  
Macaca Fascicularis ◽  
Class Ii ◽  
Class I ◽  
Major Histocompatibility ◽  
Breeding Colony ◽  
Allelic Variants ◽  
Histocompatibility Complex ◽  
Cynomolgus Macaques

AbstractMany medical advancements – including improvements to anti-rejection therapies in transplantation and vaccine development – rely on pre-clinical studies conducted in cynomolgus macaques (Macaca fascicularis). Major histocompatibility complex (MHC) class I and class II genes of cynomolgus macaques are orthologous to human leukocyte antigen complex (HLA) class I and class II genes, respectively. Both encode cell-surface proteins involved in cell recognition and rejection of non-host tissues. MHC class I and class II genes are highly polymorphic, so comprehensive genotyping requires the development of complete databases of allelic variants. Our group used PacBio circular consensus sequencing of full-length cDNA amplicons to characterize MHC class I and class II transcript sequences for a cohort of 295 Indonesian cynomolgus macaques (ICM) in a large, pedigreed breeding colony. These studies allowed us to expand the existing database of Macaca fascicularis (Mafa) alleles by identifying an additional 141 MHC class I and 61 class II transcript sequences. In addition, we defined co-segregating combinations of allelic variants as regional haplotypes for 70 Mafa-A, 78 Mafa-B and 45 Mafa-DRB gene clusters. Finally, we defined class I and class II transcripts that are associated with 100 extended MHC haplotypes in this breeding colony by combining our genotyping analyses with short tandem repeat (STR) patterns across the MHC region. Our sequencing analyses and haplotype definitions improve the utility of these ICM for transplantation studies as well as infectious disease and vaccine research.

scholarly journals MHC class I characterization of Indonesian cynomolgus macaques

2008 ◽  
Vol 60 (7) ◽  
Author(s):  
Chad J. Pendley ◽  
Ericka A. Becker ◽  
Julie A. Karl ◽  
Alex J. Blasky ◽  
Roger W. Wiseman ◽  
...  
Keyword(s):  
Mhc Class I ◽  
Class I ◽  
Cynomolgus Macaques

Influence of MHC class I and II haplotypes on the experimental infection of Mauritian cynomolgus macaques with SHIVSF162P4cy

2012 ◽  
Vol 80 (1) ◽  
pp. 36-45 ◽  
Author(s):  
A. Borsetti ◽  
M. T. Maggiorella ◽  
L. Sernicola ◽  
S. Bellino ◽  
F. Ferrantelli ◽  
...  
Keyword(s):  
Mhc Class I ◽  
Experimental Infection ◽  
Class I ◽  
Cynomolgus Macaques

scholarly journals Unusually High Frequency MHC Class I Alleles in Mauritian Origin Cynomolgus Macaques

2005 ◽  
Vol 175 (8) ◽  
pp. 5230-5239 ◽  
Author(s):  
Kendall C. Krebs ◽  
ZheYuan Jin ◽  
Richard Rudersdorf ◽  
Austin L. Hughes ◽  
David H. O’Connor
Keyword(s):  
Mhc Class I ◽  
High Frequency ◽  
Class I ◽  
Cynomolgus Macaques

Identification of 23 novel MHC class I alleles in cynomolgus macaques of Philippine and Philippine/Mauritius origins

2012 ◽  
Vol 79 (4) ◽  
pp. 306-307 ◽  
Author(s):  
J. Lawrence ◽  
D. Orysiuk ◽  
T. Prashar ◽  
R. Pilon ◽  
J. Fournier ◽  
...  
Keyword(s):  
Mhc Class I ◽  
Class I ◽  
Cynomolgus Macaques
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