scholarly journals Bacterial community profiles on feathers during composting as determined by terminal restriction fragment length polymorphism analysis of 16S rDNA genes

2004 ◽  
Vol 67 (3) ◽  
pp. 412-419 ◽  
Author(s):  
S. M. Tiquia ◽  
J. M. Ichida ◽  
H. M. Keener ◽  
D. L. Elwell ◽  
E. H. Burtt ◽  
...  
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Bacterial Community ◽  
16S Rdna ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Polymorphism Analysis ◽  
Rdna Genes ◽  
Restriction Fragment Length

Rapid Estimation of Microbial Populations in Fish Samples by Using Terminal Restriction Fragment Length Polymorphism Analysis of 16S rDNA

2010 ◽  
Vol 73 (1) ◽  
pp. 104-113 ◽  
Author(s):  
YUICHIRO TANAKA ◽  
HAJIME TAKAHASHI ◽  
NAO KITAZAWA ◽  
BON KIMURA
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
16S Rdna ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Microbial Population ◽  
Fragment Length Polymorphism ◽  
Polymorphism Analysis ◽  
Fish Samples ◽  
Restriction Fragment Length

A rapid system using terminal restriction fragment length polymorphism (T-RFLP) analysis targeting 16S rDNA is described for microbial population analysis in edible fish samples. The defined terminal restriction fragment database was constructed by collecting 102 strains of bacteria representing 53 genera that are associated with fish. Digestion of these 102 strains with two restriction enzymes, HhaI and MspI, formed 54 pattern groups with discrimination to the genus level. This TRFLP system produced results comparable to those from a culture-based method in six natural fish samples with a qualitative correspondence of 71.4 to 92.3%. Using the T-RFLP system allowed an estimation of the microbial population within 7 h. Rapid assay of the microbial population is advantageous for food manufacturers and testing laboratories; moreover, the strategy presented here allows adaptation to specific testing applications.

scholarly journals Modification of the terminal restriction fragment length polymorphism analysisfor assessment of a specific taxonomic group within a soil microbial community

2009 ◽  
Vol 55 (No. 9) ◽  
pp. 397-403 ◽  
Author(s):  
J. Kopecký ◽  
G. Novotná ◽  
M. Ságová-Marečková
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Bacterial Community ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Taxonomic Group ◽  
Polymorphism Analysis ◽  
Diversity Assessment ◽  
Restriction Fragment Length

A double color terminal restriction fragment length polymorphism analysis was applied for the diversity assessment of a complex bacterial community in a deciduous forest soil. Application of the second label was used to project a specific taxonomic group of bacteria, actinomycetes in our case, to the universal fingerprint. The diversity of actinomycetes was evaluated directly as a percentage of the whole bacterial community by comparing the corresponding terminal fragments of the same length.

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