scholarly journals Evaluation of restriction fragment length polymorphism analysis of 16S rDNA as a tool for genomovar characterisation within theBurkholderia cepaciacomplex

2002 ◽  
Vol 214 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Karen Vermis ◽  
Christoph Vandekerckhove ◽  
Hans J Nelis ◽  
Peter A.R Vandamme
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
16S Rdna ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Polymorphism Analysis ◽  
Restriction Fragment Length

Rapid Estimation of Microbial Populations in Fish Samples by Using Terminal Restriction Fragment Length Polymorphism Analysis of 16S rDNA

2010 ◽  
Vol 73 (1) ◽  
pp. 104-113 ◽  
Author(s):  
YUICHIRO TANAKA ◽  
HAJIME TAKAHASHI ◽  
NAO KITAZAWA ◽  
BON KIMURA
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
16S Rdna ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Microbial Population ◽  
Fragment Length Polymorphism ◽  
Polymorphism Analysis ◽  
Fish Samples ◽  
Restriction Fragment Length

A rapid system using terminal restriction fragment length polymorphism (T-RFLP) analysis targeting 16S rDNA is described for microbial population analysis in edible fish samples. The defined terminal restriction fragment database was constructed by collecting 102 strains of bacteria representing 53 genera that are associated with fish. Digestion of these 102 strains with two restriction enzymes, HhaI and MspI, formed 54 pattern groups with discrimination to the genus level. This TRFLP system produced results comparable to those from a culture-based method in six natural fish samples with a qualitative correspondence of 71.4 to 92.3%. Using the T-RFLP system allowed an estimation of the microbial population within 7 h. Rapid assay of the microbial population is advantageous for food manufacturers and testing laboratories; moreover, the strategy presented here allows adaptation to specific testing applications.

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