Engineering improved thermostability of the GH11 xylanase from Neocallimastix patriciarum via computational library design

2018 ◽  
Vol 102 (8) ◽  
pp. 3675-3685 ◽  
Author(s):  
Yifan Bu ◽  
Yinglu Cui ◽  
Ying Peng ◽  
Meirong Hu ◽  
Yu’e Tian ◽  
...  
Keyword(s):  
Library Design ◽  
Neocallimastix Patriciarum

Cereal grain digestion by selected strains of ruminal fungi

1993 ◽  
Vol 39 (4) ◽  
pp. 367-376 ◽  
Author(s):  
T. A. McAllister ◽  
Y. Dong ◽  
L. J. Yanke ◽  
H. D. Bae ◽  
K.-J. Cheng ◽  
...  
Keyword(s):  
Corn Starch ◽  
Fungal Growth ◽  
Wheat Starch ◽  
Cereal Grains ◽  
Starch Granules ◽  
Starch Digestion ◽  
Protein Matrix ◽  
Cereal Grain ◽  
Neocallimastix Patriciarum ◽  
Grain Starch

The ruminal fungi Orpinomyces joyonii strain 19-2, Neocallimastix patriciarum strain 27, and Piromyces communis strain 22 were examined for their ability to digest cereal starch. All strains digested corn starch more readily than barley or wheat starch. Orpinomyces joyonii 19-2 exhibited the greatest propensity to digest starch in wheat and barley, whereas the digestion of these starches by N. patriciarum 27 and P. communis 22 was limited. Media ammonia concentrations were lower when fungal growth was evident, suggesting that all strains assimilate ammonia. Fungi formed extensive rhizoidal systems on the endosperm of corn, but O. joyonii 19-2 was the only strain to form such systems on the endosperm of wheat and barley. All strains penetrated the protein matrix of corn but did not penetrate starch granules. Starch granules from all three cereals were pitted, evidence of extensive digestion by extracellular amylases produced by O. joyonii 19-2. Similar pitting was observed on the surface of corn starch granules digested by N. patriciarum 27 and P. communis 22, but not on wheat and barley starch granules. The ability of ruminal fungi to digest cereal grains depends on both the strain of fungus and the type of grain. The extent to which fungi digest cereal grain in the rumen remains to be determined.Key words: ruminal fungi, cereal grain, starch digestion, ruminant.

scholarly journals J. Max Bond Jr. and the Appropriation of Modernism in a Library Design in Ghana

Fabrications ◽  
2018 ◽  
Vol 28 (3) ◽  
pp. 355-374
Author(s):  
Allan Stephen Balaara ◽  
Errol Haarhoff ◽  
Alessandro Melis
Keyword(s):  
Library Design

Precise and Efficient Antibody Epitope Determination through Library Design, Yeast Display and Next-Generation Sequencing

2015 ◽  
Vol 427 (6) ◽  
pp. 1513-1534 ◽  
Author(s):  
Thomas Van Blarcom ◽  
Andrea Rossi ◽  
Davide Foletti ◽  
Purnima Sundar ◽  
Steven Pitts ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Library Design ◽  
Next Generation ◽  
Yeast Display ◽  
Antibody Epitope ◽  
Generation Sequencing

Informative Library Design as an Efficient Strategy to Identify and Optimize Leads:  Application to Cyclin-Dependent Kinase 2 Antagonists

2003 ◽  
Vol 46 (20) ◽  
pp. 4360-4364 ◽  
Author(s):  
Erin K. Bradley ◽  
Jennifer L. Miller ◽  
Eddine Saiah ◽  
Peter D. J. Grootenhuis
Keyword(s):  
Cyclin Dependent Kinase ◽  
Library Design ◽  
Efficient Strategy

2‐Substituted tricyclic oxazolo[5,4‐ d ]pyrimidine library: Design, synthesis and cytotoxicity activity

2021 ◽  
Author(s):  
Yan Zeng ◽  
Lifei Nie ◽  
Khurshed Bozorov ◽  
Zukela Ruzi ◽  
Buer Song ◽  
...  
Keyword(s):  
Library Design ◽  
Design Synthesis ◽  
Cytotoxicity Activity

Library design and screening protocol for artificial metalloenzymes based on the biotin-streptavidin technology

2016 ◽  
Vol 11 (5) ◽  
pp. 835-852 ◽  
Author(s):  
Hendrik Mallin ◽  
Martina Hestericová ◽  
Raphael Reuter ◽  
Thomas R Ward
Keyword(s):  
Library Design ◽  
Screening Protocol ◽  
Artificial Metalloenzymes
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