Molecular cloning of a putative divalent-cation transporter gene as a new genetic marker for the identification of Lactobacillus brevis strains capable of growing in beer

2001 ◽  
Vol 55 (5) ◽  
pp. 596-603 ◽  
Author(s):  
Nobuyuki Hayashi ◽  
Mie Ito ◽  
Satoka Horiike ◽  
Hiroshi Taguchi
Keyword(s):  
Molecular Cloning ◽  
Genetic Marker ◽  
Divalent Cation ◽  
Lactobacillus Brevis ◽  
Transporter Gene ◽  
Divalent Cation Transporter

Molecular Cloning and Bioinformatics Analysis of K+Transporter Gene (GmKT12) from Soybean (Glycine max[L．] Merri)

2013 ◽  
Vol 39 (9) ◽  
pp. 1701
Author(s):  
Xiao-Ling ZHU ◽  
Hai-Feng CHEN ◽  
Cheng WANG ◽  
Qing-Nan HAO ◽  
Li-Miao CHEN ◽  
...  
Keyword(s):  
Glycine Max ◽  
Molecular Cloning ◽  
Bioinformatics Analysis ◽  
Transporter Gene ◽  
Glycine Max L

scholarly journals The Mechanism of the Tyrosine Transporter TyrP Supports a Proton Motive Tyrosine Decarboxylation Pathway in Lactobacillus brevis

2006 ◽  
Vol 188 (6) ◽  
pp. 2198-2206 ◽  
Author(s):  
Wout A. M. Wolken ◽  
Patrick M. Lucas ◽  
Aline Lonvaud-Funel ◽  
Juke S. Lolkema
Keyword(s):  
Phenyl Ring ◽  
Lactobacillus Brevis ◽  
Proton Motive Force ◽  
Proton Pumping ◽  
Hydroxyl Group ◽  
Transporter Gene ◽  
Tyrosine Decarboxylase ◽  
Rate Analysis ◽  
Substrate Analogues ◽  
Decarboxylation Reaction

ABSTRACT The tyrosine decarboxylase operon of Lactobacillus brevis IOEB9809 contains, adjacent to the tyrosine decarboxylase gene, a gene for TyrP, a putative tyrosine transporter. The two genes potentially form a proton motive tyrosine decarboxylation pathway. The putative tyrosine transporter gene of L. brevis was expressed in Lactococcus lactis and functionally characterized using right-side-out membranes. The transporter very efficiently catalyzes homologous tyrosine-tyrosine exchange and heterologous exchange between tyrosine and its decarboxylation product tyramine. Tyrosine-tyramine exchange was shown to be electrogenic. In addition to the exchange mode, the transporter catalyzes tyrosine uniport but at a much lower rate. Analysis of the substrate specificity of the transporter by use of a set of 19 different tyrosine substrate analogues showed that the main interactions between the protein and the substrates involve the amino group and the phenyl ring with the para hydroxyl group. The carboxylate group that is removed in the decarboxylation reaction does not seem to contribute to the affinity of the protein for the substrates significantly. The properties of the TyrP protein are those typical for precursor-product exchangers that operate in proton motive decarboxylation pathways. It is proposed that tyrosine decarboxylation in L. brevis results in proton motive force generation by an indirect proton pumping mechanism.

scholarly journals Molecular cloning and expression profile of an ATP-binding cassette (ABC) transporter gene from the hemipteran insect Nilaparvata lugens

2015 ◽  
Vol 14 (1) ◽  
pp. 2654-2664 ◽  
Author(s):  
W.J. Zha ◽  
S.H. Li ◽  
L. Zhou ◽  
Z.J. Chen ◽  
K. Liu ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Abc Transporter ◽  
Expression Profile ◽  
Nilaparvata Lugens ◽  
Cloning And Expression ◽  
Atp Binding ◽  
Transporter Gene ◽  
Hemipteran Insect ◽  
Atp Binding Cassette

scholarly journals Molecular cloning and functional analysis of a sugar transporter gene (CsTST2) from cucumber (Cucumis sativus L.)

2019 ◽  
Vol 33 (1) ◽  
pp. 118-127 ◽  
Author(s):  
Bin Hu ◽  
Weifeng Huang ◽  
Liaoliao Dong ◽  
Shiqiang Liu ◽  
Yong Zhou
Keyword(s):  
Functional Analysis ◽  
Molecular Cloning ◽  
Cucumis Sativus ◽  
Sugar Transporter ◽  
Transporter Gene ◽  
Cucumis Sativus L

Molecular cloning and functional characterization of the apple sucrose transporter gene MdSUT2

2016 ◽  
Vol 109 ◽  
pp. 442-451 ◽  
Author(s):  
Qi-Jun Ma ◽  
Mei-Hong Sun ◽  
Ya-Jing Liu ◽  
Jing Lu ◽  
Da-Gang Hu ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Functional Characterization ◽  
Sucrose Transporter ◽  
Transporter Gene

Molecular cloning and structure of the human (GABATHG) GABA transporter gene

1993 ◽  
Vol 19 (3) ◽  
pp. 227-232 ◽  
Author(s):  
Dominic Man-Kit Lam ◽  
Jian Fei ◽  
Xiao-Yong Zhang ◽  
Anthony C.W. Tam ◽  
Li-Hua Zhu ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Transporter Gene ◽  
Gaba Transporter

GENE ORGANIZATION AND EXPRESSION OF THE DIVALENT CATION TRANSPORTER NRAMP IN THE PROTISTAN PARASITE PERKINSUS MARINUS

2004 ◽  
Vol 90 (5) ◽  
pp. 1004-1014 ◽  
Author(s):  
José-Antonio F. Robledo ◽  
Pascal Courville ◽  
Mathieu F. M. Cellier ◽  
Gerardo R. Vasta
Keyword(s):  
Divalent Cation ◽  
Gene Organization ◽  
Perkinsus Marinus ◽  
Divalent Cation Transporter
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