A simple and rapid Agrobacterium -mediated transformation protocol for cotton ( Gossypium hirsutum L.): Embryogenic calli as a source to generate large numbers of transgenic plants

2004 ◽  
Vol 22 (7) ◽  
pp. 465-470 ◽  
Author(s):  
S. Leelavathi ◽  
V. G. Sunnichan ◽  
R. Kumria ◽  
G. P. Vijaykanth ◽  
R. K. Bhatnagar ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Gossypium Hirsutum ◽  
Embryogenic Calli ◽  
Gossypium Hirsutum L ◽  
Transformation Protocol ◽  
Agrobacterium Mediated Transformation ◽  
Large Numbers

Transgenic Miscanthus lutarioriparius that co-expresses the Cry 2Aa# and Bar genes

2019 ◽  
Vol 99 (6) ◽  
pp. 841-851
Author(s):  
Yanan Xia ◽  
Jun Xu ◽  
Junyi Duan ◽  
Qingbo Liu ◽  
Hongmei Huang ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Transgenic Plants ◽  
Herbicide Tolerance ◽  
Transformation Method ◽  
Energy Crop ◽  
Embryogenic Calli ◽  
Transformation Protocol ◽  
Agrobacterium Mediated Transformation ◽  
Domestic Species ◽  
Miscanthus Lutarioriparius

Miscanthus lutarioriparius, a domestic species of China, belongs to the genus of perennial rhizomatous Miscanthus and is a promising energy crop for biomass production. To establish an Agrobacterium tumefaciens transformation protocol and obtain transgenic plants co-expressing the Cry 2Aa# and Bar genes, an Agrobacterium-mediated transformation method for M. lutarioriparius was developed in this study. To inhibit tissue browning, optimized callus induction and regeneration media were developed. Embryogenic calli were efficiently induced from immature inflorescences on Murashige & Skoog (MS) medium containing 0.2 g L−1 citric acid. Agrobacterium tumefaciens EHA105 carrying pC3300 with the Cry 2Aa# and Bar genes were introduced into embryogenic calli. Six transgenic lines were selected by Basta screening and confirmed by genomic PCR. The herbicide tolerance of M. lutarioriparius transgenic plants was verified by spraying with glufosinate and expression of the Cry 2Aa# protein by ELISA. Therefore, an Agrobacterium-mediated transformation protocol for M. lutarioriparius was established and the co-expression of herbicide tolerance and Cry 2Aa# was successfully introduced with the system.

Agrobacterium-mediated transformation of cotton (Gossypium hirsutum L.) with a fungal phytase gene improves phosphorus acquisition

Euphytica ◽  
2011 ◽  
Vol 181 (1) ◽  
pp. 31-40 ◽  
Author(s):  
J. F. Liu ◽  
C. Y. Zhao ◽  
J. Ma ◽  
G. Y. Zhang ◽  
M. G. Li ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Phosphorus Acquisition ◽  
Gossypium Hirsutum L ◽  
Phytase Gene ◽  
Agrobacterium Mediated Transformation

Induction of Highly Embryogenic Calli and Plant Regeneration in Upland ( Gossypium hirsutum L.) and Pima ( Gossypium barbadense L.) Cottons

Crop Science ◽  
2001 ◽  
Vol 41 (4) ◽  
pp. 1235-1240 ◽  
Author(s):  
Hamidou F. Sakhanokho ◽  
Allan Zipf ◽  
Kanniah Rajasekaran ◽  
Sukumar Saha ◽  
Govind C. Sharma
Keyword(s):  
Plant Regeneration ◽  
Gossypium Hirsutum ◽  
Gossypium Barbadense ◽  
Embryogenic Calli ◽  
Gossypium Hirsutum L

scholarly journals Optimization of the parameters of Agrobacterium-mediated transformation of bar-shaped millet

2020 ◽  
pp. 32-43
Author(s):  
A.T. Zhumabek ◽  
◽  
Y.M. Ramankulov ◽  
S.A. Manabayeva ◽  
◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Optical Density ◽  
Panicum Virgatum ◽  
Virulence Gene ◽  
Transformation Method ◽  
Bacterial Suspension ◽  
Embryogenic Calli ◽  
Agrobacterium Mediated Transformation ◽  
Agrobacterial Transformation ◽  
Panicum Virgatum L

An efficient Agrobacterium-mediated transformation method has been developed and optimized for switchgrass (Panicum virgatum L.). The aim of this study is to optimize the conditions for agrobacterial transformation of upland switchgrass cultivars Alamo, Forestburg, Pathfinder, Shawnee, and Trailblazer. For genetic transformation, the authors have used embryogenic calli of switchgrass cultivars. As a result of the study the effect of the virulence gene inducer, surfactant, optical density of bacterial suspension and co-cultivation time, it has been revealed that the presence of acetosyringone with silwet-L77 in the medium, bacterial suspension at an optical density of 0.6 and 8 days of co-cultivation with bacteria is optimal for Agrobacterium– mediated transformation of embryogenic calli of switchgrass. The results obtained can serve as a basis for the creation of transgenic plants of switchgrass.

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