Effect of ticarcillin/potassium clavulanate on callus growth and shoot regeneration in Agrobacterium -mediated transformation of tomato ( Lycopersicon esculentum Mill.)

1998 ◽  
Vol 17 (11) ◽  
pp. 843-847 ◽  
Author(s):  
H.-Q. Ling ◽  
D. Kriseleit ◽  
M. W. Ganal
Keyword(s):  
Lycopersicon Esculentum ◽  
Shoot Regeneration ◽  
Callus Growth ◽  
Agrobacterium Mediated Transformation ◽  
Lycopersicon Esculentum Mill ◽  
Potassium Clavulanate

scholarly journals The effect of gamma radiation on callus growth and plantlet regeneration of four tomato hybrid Lycopersicon esculentum mill In vitro

2009 ◽  
Vol 3 (1) ◽  
pp. 56-62
Author(s):  
AssRana A.H. Al-roomia
Keyword(s):  
Lycopersicon Esculentum ◽  
Gamma Radiation ◽  
Dry Weight ◽  
Plantlet Regeneration ◽  
Callus Growth ◽  
Control Treatment ◽  
Ms Medium ◽  
Lycopersicon Esculentum Mill

Calluses were induced from four tomato hybrid (Davista, superDavista, Muntcarloo,Hymar). On MS medium supplemented with 2, 4-D Auxin at concentration of 4 mg/L.The Callus were divided into two groups the first one was affected with gammaradiation at the doses (0,2,4,6,8)Gray and re cultured on the same mentioned medium.The second group was also affected with gamma radiation with the same dosesmentioned above and re- cultured on MS media supplemented with 0.5 mg/L Kinetinfor regeneration. Fresh and dry weight was measured after 8 weeks. The number andlength of shoots were measured as indicators after 6 weeks. Results revealed that thedose 2 Gray was superior to give highest rate of fresh and dry weight reaching 238.5and 43.1 mg respectively and the hybrid super Davista was superior in giving thehighest fresh and dry weight reaching 231.6 and 30.2 mg respectively. Moreover theresults revealed that control treatment was superior in giving the highest rate innumber and length of plantlets. The 2 Gray treatment showed a significant effect onthe rate and length of plantlets. In addition, there were significant differences amongthe cultivars in most characters mentioned above.

Shoot regeneration of tomato (Lycopersicon esculentum Mill.) in tissue culture using several kinds of supporting materials

Plant Science ◽  
1995 ◽  
Vol 108 (1) ◽  
pp. 93-100 ◽  
Author(s):  
Kazuo Ichimura ◽  
Toshiko Uchiumi ◽  
Kenkou Tsuji ◽  
Masayuki Oda ◽  
Masaaki Nagaoka
Keyword(s):  
Tissue Culture ◽  
Lycopersicon Esculentum ◽  
Shoot Regeneration ◽  
Lycopersicon Esculentum Mill ◽  
Supporting Materials

scholarly journals Effect of Genotype and Explant Type on Shoot Regeneration in Tomato (Lycopersicon esculentum Mill.) in vitro

2011 ◽  
Vol 39 (No. 1) ◽  
pp. 9-14 ◽  
Author(s):  
J. Gubiš ◽  
Z. Lajchová ◽  
J. Faragó ◽  
Z. Jureková
Keyword(s):  
Acetic Acid ◽  
Lycopersicon Esculentum ◽  
Shoot Regeneration ◽  
Explant Type ◽  
Regeneration Capacity ◽  
Regeneration Medium ◽  
Lycopersicon Esculentum Mill ◽  
Shoot Primordia ◽  
Indole 3 Acetic Acid

The regeneration capacity of six types of explants (segments from hypocotyl, cotyledons, epicotyl, leaf, internodes and petiole) was compared in 13 cultivars of tomato (Lycopersicon esculentum Mill). Explants were cultured on a regeneration medium containing 1 mg/l zeatin and 0.1 mg/l indole-3-acetic acid. The number of shoot primordia and shoots with 1 or more fully developed leaves was evaluated after 6 weeks. The regeneration capacity was significantly influenced by cultivars and explant types. The total number of shoot primordia produced in all types of explants was highest in the cultivars Hana and Premium and lowest in UC 82 and Money Marker. Cv. Hana also produced the highest number of shoots. The most responsive explants in most cultivars were hypocotyls and epicotyls with up to 100% regeneration and mean production of 6.3 and 6.5 shoot primordia per explant, respectively.  

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