An updated genetic map of Peromyscus with chromosomal assignment of linkage groups

A molecular-marker linkage map has been constructed for perennial ryegrass (Lolium perenne L.) using a one-way pseudo-testcross population based on the mating of a multiple heterozygous individual with a doubled haploid genotype. RFLP, AFLP, isoenzyme, and EST data from four collaborating laboratories within the International Lolium Genome Initiative were combined to produce an integrated genetic map containing 240 loci covering 811 cM on seven linkage groups. The map contained 124 codominant markers, of which 109 were heterologous anchor RFLP probes from wheat, barley, oat, and rice, allowing comparative relationships between perennial ryegrass and other Poaceae species to be inferred. The genetic maps of perennial ryegrass and the Triticeae cereals are highly conserved in terms of synteny and colinearity. This observation was supported by the general agreement of the syntenic relationships between perennial ryegrass, oat, and rice and those between the Triticeae and these species. A lower level of synteny and colinearity was observed between perennial ryegrass and oat compared with the Triticeae, despite the closer taxonomic affinity between these species. It is proposed that the linkage groups of perennial ryegrass be numbered in accordance with these syntenic relationships, to correspond to the homoeologous groups of the Triticeae cereals.Key words: Lolium perenne, genetic linkage map, RFLP, AFLP, conserved synteny.

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Genetic map of eight microsatellite markers comprising two linkage groups on rat chromosome 6

Cytogenetic and Genome Research ◽

10.1159/000133901 ◽

1995 ◽

Vol 68 (1-2) ◽

pp. 107-111 ◽

Cited By ~ 4

Author(s):

Y. Du ◽

E.F. Remmers ◽

H. Zha ◽

E.A. Goldmuntz ◽

P. Mathern ◽

...

Keyword(s):

Microsatellite Markers ◽

Genetic Map ◽

Chromosome 6 ◽

Linkage Groups

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Centromere-Linkage Analysis and Consolidation of the Zebrafish Genetic Map

Genetics ◽

10.1093/genetics/142.4.1277 ◽

1996 ◽

Vol 142 (4) ◽

pp. 1277-1288

Author(s):

Stephen L Johnson ◽

Michael A Gates ◽

Michele Johnson ◽

William S Talbot ◽

Sally Horne ◽

...

Keyword(s):

Linkage Group ◽

Linkage Analysis ◽

Genetic Analysis ◽

Linkage Map ◽

Rapid Method ◽

Genetic Map ◽

Dna Polymorphisms ◽

Linkage Groups

Abstract The ease of isolating mutations in zebrafish will contribute to an understanding of a variety of processes common to all vertebrates. To facilitate genetic analysis of such mutations, we have identified DNA polymorphisms closely linked to each of the 25 centromeres of zebrafish, placed centromeres on the linkage map, increased the number of mapped PCR-based markers to 652, and consolidated the number of linkage groups to the number of chromosomes. This work makes possible centromere-linkage analysis, a novel, rapid method to assign mutations to a specific linkage group using half-tetrads.

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Genetic map of 16 polymorphic markers forming three linkage groups assigned to rat Chromosome 4

Mammalian Genome ◽

10.1007/bf00360654 ◽

1995 ◽

Vol 6 (7) ◽

pp. 459-463 ◽

Cited By ~ 7

Author(s):

E. A. Goldmuntz ◽

E. F. Remmers ◽

Y. Du ◽

H. Zha ◽

P. Mathern ◽

...

Keyword(s):

Genetic Map ◽

Polymorphic Markers ◽

Chromosome 4 ◽

Linkage Groups

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Chromosomal regions which control sporulation in Bacillus subtilis

Canadian Journal of Microbiology ◽

10.1139/m69-137 ◽

1969 ◽

Vol 15 (7) ◽

pp. 787-790 ◽

Cited By ~ 6

Author(s):

Marvin Rogolsky

Keyword(s):

Bacillus Subtilis ◽

Linkage Group ◽

Genetic Map ◽

Gene Clusters ◽

Linkage Groups ◽

The Third ◽

The Right

Large quantities of sporulation mutants have been isolated with a variety of mutagens. The genetic sites for asporogeny have been localized on the chromosome of Bacillus subtilis through transduction with phage PBSI. Through these procedures specific portions of the chromosome which are associated with sporulation have been identified. Although asporogenic (Sp−) defects were observed to be scattered throughout the four linkage groups of the genetic map of B. subtilis, only three extensive Sp− linkage groups were identified. The first linkage group of Sp− markers is located at the proximal end of the chromosome between the cys A and ery markers. The second cluster of spore genes mapped to the right of ura, and the third linkage group of spore markers mapped to the left of lys-2. Defects within specific regions of the first and third spore gene clusters obstructed some early products of sporogenesis.

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Integration of the Cytogenetic and Genetic Linkage Maps of Brassica oleracea

Genetics ◽

10.1093/genetics/161.3.1225 ◽

2002 ◽

Vol 161 (3) ◽

pp. 1225-1234 ◽

Cited By ~ 5

Author(s):

Elaine C Howell ◽

Guy C Barker ◽

Gareth H Jones ◽

Michael J Kearsey ◽

Graham J King ◽

...

Keyword(s):

Linkage Map ◽

Brassica Oleracea ◽

Genetic Map ◽

Genetic Linkage ◽

Linkage Maps ◽

Linkage Groups ◽

Opposite Orientation ◽

Cytogenetic Map ◽

Specific Pcr ◽

Genetic Linkage Maps

Abstract We have assigned all nine linkage groups of a Brassica oleracea genetic map to each of the nine chromosomes of the karyotype derived from mitotic metaphase spreads of the B. oleracea var. alboglabra line A12DHd using FISH. The majority of probes were BACs, with A12DHd DNA inserts, which give clear, reliable FISH signals. We have added nine markers to the existing integrated linkage map, distributed over six linkage groups. BACs were definitively assigned to linkage map positions through development of locus-specific PCR assays. Integration of the cytogenetic and genetic linkage maps was achieved with 22 probes representing 19 loci. Four chromosomes (2, 4, 7, and 9) are in the same orientation as their respective linkage groups (O4, O7, O8, and O6) whereas four chromosomes (1, 3, 5, and 8) and linkage groups (O3, O9, O2, and O1) are in the opposite orientation. The remaining chromosome (6) is probably in the opposite orientation. The cytogenetic map is an important resource for locating probes with unknown genetic map positions and is also being used to analyze the relationships between genetic and cytogenetic maps.

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An SSR genetic map of Sorghum bicolor (L.) Moench and its comparison to a published genetic map

Genome ◽

10.1139/g06-133 ◽

2007 ◽

Vol 50 (1) ◽

pp. 84-89 ◽

Cited By ~ 27

Author(s):

Y.Q. Wu ◽

Yinghua Huang

Keyword(s):

Sorghum Bicolor ◽

Genetic Map ◽

Dna Markers ◽

Genetic Distances ◽

Genetic Maps ◽

Linkage Groups ◽

Common Marker ◽

Ssr Loci ◽

The Difference ◽

Sorghum Bicolor L

Sorghum bicolor (L.) Moench is an important grain and forage crop grown worldwide. We developed a simple sequence repeat (SSR) linkage map for sorghum using 352 publicly available SSR primer pairs and a population of 277 F2 individuals derived from a cross between the Westland A line and PI 550610. A total of 132 SSR loci appeared polymorphic in the mapping population, and 118 SSRs were mapped to 16 linkage groups. These mapped SSR loci were distributed throughout 10 chromosomes of sorghum, and spanned a distance of 997.5 cM. More important, 38 new SSR loci were added to the sorghum genetic map in this study. The mapping result also showed that chromosomes SBI-01, SBI-02, SBI-05, and SBI-06 each had 1 linkage group; the other 6 chromosomes were composed of 2 linkage groups each. Except for 5 closely linked marker flips and 1 locus (Sb6_34), the marker order of this map was collinear to a published sorghum map, and the genetic distances of common marker intervals were similar, with a difference ratio ≤ 0.05 between the 2 maps. The difference ratio is a new index developed in this study that can be used to compare the genetic distances of DNA markers between 2 maps. This SSR map carrying additional SSR markers will facilitate mapping quantitative trait loci to the sorghum genome and map-based gene cloning. Furthermore, the novel method for calculating distance between DNA markers will be a useful tool for the comparative analysis of genetic markers between linkage maps with different genetic backgrounds and the alignment of different sorghum genetic maps.

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Construction of a reference linkage map for melon

Genome ◽

10.1139/g01-073 ◽

2001 ◽

Vol 44 (5) ◽

pp. 836-845 ◽

Cited By ~ 45

Author(s):

M Oliver ◽

J Garcia-Mas ◽

M Cardús ◽

N Pueyo ◽

A I López-Sesé ◽

...

Keyword(s):

Molecular Markers ◽

Genetic Distance ◽

Linkage Map ◽

Genetic Map ◽

Cucumis Melo ◽

Morphological Trait ◽

Linkage Groups ◽

Resistant Gene ◽

Anchor Points ◽

Cucumis Melo L

A map of melon (Cucumis melo L.) with 411 markers (234 RFLPs, 94 AFLPs, 47 RAPDs, 29 SSRs, five inter-SSRs, and two isozymes) and one morphological trait (carpel number) was constructed using the F2 progeny of a cross between the Korean accession PI161375 and the Spanish melon type 'Pinyonet Piel de Sapo'. RFLPs were obtained using 212 probes from different genomic and cDNA melon libraries, including 16 Arabidopsis ESTs, 13 Cucumis known genes, and three resistant gene homologues. Most loci (391) mapped to 12 major linkage groups, spanning a total genetic distance of 1197 cM, with an average map interval of 3 cM/marker. The remaining 21 loci (six RAPDs and 15 AFLPs) were not linked. A majority (66%) of the markers were codominant (RFLPs, SSRs, and isozymes), making them easily transferable to other melon crosses. Such markers can be used as a reference, to merge other melon and cucumber maps already constructed. Indeed, some of them (23 SSRs, 14 RFLPs, one isozyme, and one morphological trait) could act as anchor points with other published cucurbit maps.Key words: Cucumis melo, genetic map, molecular markers, RFLPs, SSRs.

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A Molecular Genetic Map and Electrophoretic Karyotype of the Plant Pathogenic Fungus Cochliobolus sativus

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi.2002.15.5.481 ◽

2002 ◽

Vol 15 (5) ◽

pp. 481-492 ◽

Cited By ~ 48

Author(s):

Shaobin Zhong ◽

Brian J. Steffenson ◽

J. Patrick Martinez ◽

Lynda M. Ciuffetti

Keyword(s):

Genetic Map ◽

Length Polymorphism ◽

Fragment Length Polymorphism ◽

Molecular Genetic ◽

Aflp Markers ◽

Cochliobolus Sativus ◽

Electrophoretic Karyotype ◽

Linkage Groups ◽

Molecular Genetic Map ◽

Map Distance

A molecular genetic map was constructed and an electrophoretic karyotype was resolved for Cochliobolus sativus, the causal agent of spot blotch of barley and wheat. The genetic map consists of 27 linkage groups with 97 amplified fragment length polymorphism (AFLP) markers, 31 restriction fragment length polymorphism (RFLP) markers, two polymerase chain reaction amplified markers, the mating type locus (CsMAT), and a gene (VHv1) conditioning high virulence on barley cv. Bowman. These linkage groups covered a map distance of 849 cM. The virulence gene VHv1 cosegregated with six AFLP markers and was mapped on one of the major linkage groups. Fifteen chromosome-sized DNAs were resolved in C. sativus isolates ND93-1 and ND90Pr with contour-clamped homogeneous electric field (CHEF) electrophoresis combined with telo-mere probe analysis of comigrating chromosome-sized DNAs. The chromosome sizes ranged from 1.25 to 3.80 Mbp, and the genome size of the fungus was estimated to be approximately 33 Mbp. By hybridizing genetically mapped RFLP and AFLP markers to CHEF blots, 25 of the 27 linkage groups were assigned to specific chromosomes. The barley-specific virulence locus VHv1 was localized on a chromosome of 2.80 Mbp from isolate ND90Pr in the CHEF gel. The total map length of the fungus was estimated to be at least 1,329 cM based on the map distance covered by the linked markers and the estimated gaps. Therefore, the physical to genetic distance ratio is approximately 25 kb/cM. Construction of a high-resolution map around target loci will facilitate the cloning of the genes conferring virulence and other characters in C. sativus by a map-based cloning strategy.

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