In silico evaluation and in vitro growth inhibition of Plasmodium falciparum by natural amides and synthetic analogs

2020 ◽  
Vol 119 (6) ◽  
pp. 1879-1887
Author(s):  
Minelly Azevedo da Silva ◽  
Márcia Paranho Veloso ◽  
Kassius de Souza Reis ◽  
Guilherme de Matos Passarini ◽  
Ana Paula de Azevedo dos Santos ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Growth Inhibition ◽  
In Silico ◽  
In Vitro Growth ◽  
Synthetic Analogs

scholarly journals Correction to: In silico evaluation and in vitro growth inhibition of Plasmodium falciparum by natural amides and synthetic analogs

2020 ◽  
Vol 119 (6) ◽  
pp. 1889-1889
Author(s):  
Minelly Azevedo da Silva ◽  
Márcia Paranho Veloso ◽  
Kassius de Souza Reis ◽  
Guilherme Matos Passarini ◽  
Ana Paula de Azevedo dos Santos ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Growth Inhibition ◽  
In Silico ◽  
In Vitro Growth ◽  
Synthetic Analogs

scholarly journals Human-human hybridomas secreting monoclonal antibodies to the Mr 195,000 Plasmodium falciparum blood stage antigen.

1986 ◽  
Vol 163 (1) ◽  
pp. 179-188 ◽  
Author(s):  
R Schmidt-Ullrich ◽  
J Brown ◽  
H Whittle ◽  
P S Lin
Keyword(s):  
Plasmodium Falciparum ◽  
Growth Inhibition ◽  
Protective Immunity ◽  
Lymphoblastoid Cell Line ◽  
In Vitro Growth ◽  
Human Lymphoblastoid Cell ◽  
Immune Precipitation ◽  
Human Lymphoblastoid Cell Line ◽  
Donor Lymphocytes

Using the human lymphoblastoid cell line, GM 4672, and PBL of Gambian adults immune to Plasmodium falciparum (Pf) malaria, we have produced human-human hybridomas and selected those that produce mAb against Pf antigens. The fusion frequency, using PWM-stimulated donor lymphocytes was between 6.8 X 10(-5) and 1.5 X 10(-6). Using immune fluorescence, immune precipitation, and Pf in vitro growth inhibition, we cloned four hybridomas that reacted with the Pf Mr 195,000 schizont/merozoite protein. The differences in proteins immune precipitated and in growth inhibition indicate that, during development of protective immunity against Pf malaria, a spectrum of antibodies is produced reacting with different epitopes on the same antigen. Only a portion of these antibodies exhibits biological activity, suggesting that the recognition of certain epitopes is required for the development of a protective immune response.

scholarly journals Development of fluorescent Plasmodium falciparum for in vitro growth inhibition assays

2010 ◽  
Vol 9 (1) ◽  
pp. 152 ◽  
Author(s):  
Danny W Wilson ◽  
Brendan S Crabb ◽  
James G Beeson
Keyword(s):  
Plasmodium Falciparum ◽  
Growth Inhibition ◽  
In Vitro Growth

ANTIBODY-MEDIATED IN VITRO GROWTH INHIBITION OF FIELD ISOLATES OF PLASMODIUM FALCIPARUM FROM ASYMPTOMATIC CHILDREN IN BURKINA FASO

2003 ◽  
Vol 68 (6) ◽  
pp. 728-733 ◽  
Author(s):  
AHMED BOLAD ◽  
ALFRED TRAORE ◽  
KLAVS BERZINS ◽  
NADINE CUZIN-OUATTARA ◽  
ISSA NEBIÉ ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Growth Inhibition ◽  
Burkina Faso ◽  
In Vitro Growth ◽  
Field Isolates ◽  
Asymptomatic Children

In Vitro Growth Inhibition of Plasmodium Falciparum by Sera from Tropical Splenomegaly Syndrome Patients

1986 ◽  
Vol 35 (4) ◽  
pp. 708-710
Author(s):  
James R. Campbell ◽  
Liliana Kurniawan ◽  
Budhi Leksana ◽  
Harijani A. Marwoto ◽  
Stephen L. Hoffman
Keyword(s):  
Plasmodium Falciparum ◽  
Growth Inhibition ◽  
In Vitro Growth

In Vitro Growth Inhibition of Plasmodium falciparum by Sera from Different Regions of the Philippines

1990 ◽  
Vol 43 (3) ◽  
pp. 243-247 ◽  
Author(s):  
Nunilon E. Sy ◽  
Shirley F. Cruzada ◽  
Richard B. Oberst ◽  
Larry W. Laughlin ◽  
Victoria D. Fallarme ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Growth Inhibition ◽  
The Philippines ◽  
In Vitro Growth

Plasmodium falciparum : in vitro growth inhibition by febrile temperatures

2001 ◽  
Vol 87 (7) ◽  
pp. 553-555 ◽  
Author(s):  
Not Available Not Available ◽  
Not Available Not Available ◽  
Not Available Not Available ◽  
Not Available Not Available
Keyword(s):  
Plasmodium Falciparum ◽  
Growth Inhibition ◽  
In Vitro Growth

scholarly journals Stage-dependent effect of deferoxamine on growth of Plasmodium falciparum in vitro

Blood ◽  
1990 ◽  
Vol 76 (6) ◽  
pp. 1250-1255 ◽  
Author(s):  
S Whitehead ◽  
TE Peto
Keyword(s):  
Plasmodium Falciparum ◽  
Growth Inhibition ◽  
Antimalarial Activity ◽  
Clinical Malaria ◽  
Inhibitory Effect ◽  
Parasite Development ◽  
And Control ◽  
Speed Of Onset

Abstract Deferoxamine (DF) has antimalarial activity that can be demonstrated in vitro and in vivo. This study is designed to examine the speed of onset and stage dependency of growth inhibition by DF and to determine whether its antimalarial activity is cytostatic or cytocidal. Growth inhibition was assessed by suppression of hypoxanthine incorporation and differences in morphologic appearance between treated and control parasites. Using synchronized in vitro cultures of Plasmodium falciparum, growth inhibition by DF was detected within a single parasite cycle. Ring and nonpigmented trophozoite stages were sensitive to the inhibitory effect of DF but cytostatic antimalarial activity was suggested by evidence of parasite recovery in later cycles. However, profound growth inhibition, with no evidence of subsequent recovery, occurred when pigmented trophozoites and early schizonts were exposed to DF. At this stage in parasite development, the activity of DF was cytocidal and furthermore, the critical period of exposure may be as short as 6 hours. These observations suggest that iron chelators may have a role in the treatment of clinical malaria.

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