Enhancement of bacteriophage λ stability using a λQ − S − mutant in the continuous culture of Escherichia coli

2010 ◽  
Vol 33 (9) ◽  
pp. 1103-1107 ◽  
Author(s):  
Jeong Seok Oh ◽  
Shin Sik Choi ◽  
Tai Hyun Park
Keyword(s):  
Escherichia Coli ◽  
Continuous Culture ◽  
Bacteriophage Λ

Restriction of bacteriophage λ by Escherichia coli K

1973 ◽  
Vol 81 (3) ◽  
pp. 395-407 ◽  
Author(s):  
Noreen E. Murray ◽  
P.L. Batten ◽  
K. Murray
Keyword(s):  
Escherichia Coli ◽  
Bacteriophage Λ

scholarly journals THE INTERACTION OF cos WITH CHI IS SEPARABLE FROM DNA PACKAGING IN recA-recBC-MEDIATED RECOMBINATION OF BACTERIOPHAGE LAMBDA

Genetics ◽  
1983 ◽  
Vol 104 (4) ◽  
pp. 549-570
Author(s):  
Ichizo Kobayashi ◽  
Mary M Stahl ◽  
David Leach ◽  
Franklin W Stahl
Keyword(s):  
Escherichia Coli ◽  
Bacteriophage Lambda ◽  
Dna Packaging ◽  
Bacteriophage Λ ◽  
Entry Site ◽  
Two Component ◽  
General Recombination

ABSTRACT Chi (5′-GCTGGTGG) is a recombinator in RecA- RecBC-mediated recombination in Escherichia coli. In bacteriophage λ vegetative recombination, Chi is fully active only when it is correctly oriented with respect to cos, the site that defines the ends of the packaged chromosome. Here we demonstrate that packaging from cos is not necessary for this cos-Chi interaction. Our evidence suggests that correctly oriented cos is an activator of Chi. cos, as an activator, is (1) dominant over cos  -, (2) active opposite an extensive heterology, (3) able to interact with Chi only when on the same (cis) chromosome, and (4) able to interact with Chi at distances as far as ≥ 20 kb. Thus, cos and Chi form a two-component recombinator system for general recombination. cos may serve as an asymmetric entry site for a recombination enzyme that recognizes Chi in an asymmetric way.

scholarly journals Investigating the use of bacteriophages as a new decolonization strategy for intestinal carriage of CTX-M-15-producing ST131 Escherichia coli: An in vitro continuous culture system model

2020 ◽  
Vol 22 ◽  
pp. 664-671
Author(s):  
Odette J. Bernasconi ◽  
Edgar I. Campos-Madueno ◽  
Valentina Donà ◽  
Vincent Perreten ◽  
Alessandra Carattoli ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Continuous Culture ◽  
Culture System ◽  
System Model ◽  
Continuous Culture System
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