Globin gene structure in a reptile supports the transpositional model for amniote α- and β-globin gene evolution

In previous work, three clonal cell lines with extremely low DNA methylation levels were derived by multiple consecutive treatments of C3H 10T1/2 C18 (10T1/2) cells with 5-aza-2'-deoxycytidine (5-aza-CdR). In this study we examined the methylation status of genes in these three methyl-deficient clones to assess the specificity of the induced hypomethylation. Complete demethylation of virtually all 5'-CCGG-3' sites was observed in four genes examined, but some sites common to all three clones were persistently methylated even after further exhaustive 5-aza-CdR treatment. Thus, there is a subset of methylation sites within these cells which can never be stably demethylated. The extensive demethylation was not always associated with changes in the level of RNA expression of the genes examined but was strongly correlated with an altered chromatin structure of the unexpressed alpha 1-globin gene and the muscle determination gene MyoD1. These results provide a direct correlation between hypomethylation and the induction of a transcriptionally competent chromatin state.

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The Globin Gene: Structure and Expression

Cold Spring Harbor Symposia on Quantitative Biology ◽

10.1101/sqb.1974.038.01.090 ◽

1974 ◽

Vol 38 (0) ◽

pp. 885-890 ◽

Cited By ~ 40

Author(s):

J. Paul ◽

R. S. Gilmour ◽

N. Affara ◽

G. Birnie ◽

P. Harrison ◽

...

Keyword(s):

Gene Structure ◽

Globin Gene ◽

Gene Structure And Expression

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Analysis of Globin Gene Structure in Patients with β Thalassemia by Restriction Endonuclease Mapping

Hemoglobin ◽

10.3109/03630268108997545 ◽

1981 ◽

Vol 5 (3) ◽

pp. 209-215 ◽

Cited By ~ 3

Author(s):

Joseph W. O. Tam ◽

Russel E. Kaufman ◽

Arthur W. Nienhuis

Keyword(s):

Restriction Endonuclease ◽

Gene Structure ◽

Globin Gene ◽

Restriction Endonuclease Mapping ◽

Endonuclease Mapping

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Biogeography and adaptation of Notothenioid fish: Hemoglobin function and globin–gene evolution

Gene ◽

10.1016/j.gene.2007.02.047 ◽

2007 ◽

Vol 398 (1-2) ◽

pp. 143-155 ◽

Cited By ~ 42

Author(s):

Guido di Prisco ◽

Joseph T. Eastman ◽

Daniela Giordano ◽

Elio Parisi ◽

Cinzia Verde

Keyword(s):

Gene Evolution ◽

Globin Gene ◽

Notothenioid Fish ◽

Fish Hemoglobin

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Gene structure and transcription in mouse cells with extensively demethylated DNA.

Molecular and Cellular Biology ◽

10.1128/mcb.9.3.885 ◽

1989 ◽

Vol 9 (3) ◽

pp. 885-892 ◽

Cited By ~ 28

Author(s):

L A Michalowsky ◽

P A Jones

Keyword(s):

Dna Methylation ◽

Cell Lines ◽

Gene Structure ◽

Chromatin Structure ◽

Globin Gene ◽

Methylation Status ◽

Rna Expression ◽

Strongly Correlated ◽

Clonal Cell Lines ◽

Mouse Cells

In previous work, three clonal cell lines with extremely low DNA methylation levels were derived by multiple consecutive treatments of C3H 10T1/2 C18 (10T1/2) cells with 5-aza-2'-deoxycytidine (5-aza-CdR). In this study we examined the methylation status of genes in these three methyl-deficient clones to assess the specificity of the induced hypomethylation. Complete demethylation of virtually all 5'-CCGG-3' sites was observed in four genes examined, but some sites common to all three clones were persistently methylated even after further exhaustive 5-aza-CdR treatment. Thus, there is a subset of methylation sites within these cells which can never be stably demethylated. The extensive demethylation was not always associated with changes in the level of RNA expression of the genes examined but was strongly correlated with an altered chromatin structure of the unexpressed alpha 1-globin gene and the muscle determination gene MyoD1. These results provide a direct correlation between hypomethylation and the induction of a transcriptionally competent chromatin state.

Download Full-text