scholarly journals Development of a chemically defined platform fed-batch culture media for monoclonal antibody-producing CHO cell lines with optimized choline content

2018 ◽  
Vol 70 (3) ◽  
pp. 939-948 ◽  
Author(s):  
Shinobu Kuwae ◽  
Ichiko Miyakawa ◽  
Tomohiro Doi
Keyword(s):  
Monoclonal Antibody ◽  
Batch Culture ◽  
Cell Lines ◽  
Culture Media ◽  
Fed Batch ◽  
Cho Cell ◽  
Fed Batch Culture

Bax and Bak knockout apoptosis‐resistant CHO cell lines significantly improve culture viability and titer in intensified fed‐batch culture process

2021 ◽  
Author(s):  
Danming Tang ◽  
Cynthia Lam ◽  
Niels Bauer ◽  
Simon Auslaender ◽  
Brad Snedecor ◽  
...  
Keyword(s):  
Batch Culture ◽  
Cell Lines ◽  
Fed Batch ◽  
Cho Cell ◽  
Culture Viability ◽  
Culture Process ◽  
Fed Batch Culture

scholarly journals Selection of chemically defined media for CHO cell fed-batch culture processes

2016 ◽  
Vol 69 (1) ◽  
pp. 39-56 ◽  
Author(s):  
Xiao Pan ◽  
Mathieu Streefland ◽  
Ciska Dalm ◽  
René H. Wijffels ◽  
Dirk E. Martens
Keyword(s):  
Batch Culture ◽  
Fed Batch ◽  
Cho Cell ◽  
Chemically Defined Media ◽  
Defined Media ◽  
Fed Batch Culture ◽  
Selection Of

Principle of Express Selection of Leading Producing Clones of Monoclonal Antibodies in the Development of Stable CHO-Based Cell Lines

2019 ◽  
Vol 35 (4) ◽  
pp. 65-72
Author(s):  
V.I. Pavelko ◽  
I.A. Kirik ◽  
V.N. Bade ◽  
T.O. Malygina ◽  
R.A. Khamitov ◽  
...  
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Parallel Processing ◽  
Cell Lines ◽  
Cho Cells ◽  
Fed Batch ◽  
Cho Cell ◽  
Recombinant Monoclonal Antibody ◽  
Selection Of

Growth and productive characteristics of monoclonal cell lines based on CHO cells and producing a therapeutic protein have been monitored using the robot Ambr Tap Biosystems, which permitted to identify the leading line. Twenty four clones producing a recombinant monoclonal antibody were studied under the close to industrial conditions in a fed-batch culturing mode. The ambr®15 cell culture workstation controls 24 disposable mini bioreactors, and offers parallel processing and evaluation of multiple (24) experiments in an automated bench-top system. The volumetric productivity of 24 clones determined by ELISA was 120-450 mg /L. A protocol was shown to select a leader among producing clones for further research. producing clones, mini bioreactor; Ambr Tap Biosystems, fed-batch, monoclonal antibodies, CHO cell culture

Protein-free fed-batch culture of non-GS NS0 cell lines for production of recombinant antibodies

2006 ◽  
Vol 96 (2) ◽  
pp. 281-293 ◽  
Author(s):  
John E. Burky ◽  
Mark C. Wesson ◽  
Amy Young ◽  
Sharyn Farnsworth ◽  
Ben Dionne ◽  
...  
Keyword(s):  
Batch Culture ◽  
Cell Lines ◽  
Recombinant Antibodies ◽  
Fed Batch ◽  
Fed Batch Culture

Maximizing productivity of CHO cell-based fed-batch culture using chemically defined media conditions and typical manufacturing equipment

2010 ◽  
Vol 26 (5) ◽  
pp. 1400-1410 ◽  
Author(s):  
Yao-Ming Huang ◽  
WeiWei Hu ◽  
Eddie Rustandi ◽  
Kevin Chang ◽  
Helena Yusuf-Makagiansar ◽  
...  
Keyword(s):  
Batch Culture ◽  
Fed Batch ◽  
Cho Cell ◽  
Manufacturing Equipment ◽  
Chemically Defined Media ◽  
Defined Media ◽  
Fed Batch Culture

Enhancement of monoclonal antibody yield by hybridoma fed-batch culture, resulting in extended maintenance of viable cell population

1994 ◽  
Vol 44 (9) ◽  
pp. 1099-1106 ◽  
Author(s):  
M. E. Bushell ◽  
S. L. Bell ◽  
M. F. Scott ◽  
R. E. Spier ◽  
J. N. Wardell ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Batch Culture ◽  
Cell Population ◽  
Viable Cell ◽  
Fed Batch ◽  
Fed Batch Culture

Application of improved top-down approach in maximizing CHO cell mass and productivity in fed-batch culture

2013 ◽  
Vol 88 (7) ◽  
pp. 1237-1247 ◽  
Author(s):  
Ya-Ting Sun ◽  
Liang Zhao ◽  
Xu-Ping Liu ◽  
Dong-dong Hu ◽  
Li Fan ◽  
...  
Keyword(s):  
Batch Culture ◽  
Cell Mass ◽  
Fed Batch ◽  
Cho Cell ◽  
Top Down ◽  
Fed Batch Culture
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