The influence of plant growth regulators and storage on root induction and growth in Pelargonium zonale cuttings

The Almond (Amygdalus communis) is one of the most important and oldest commercial nut crops, belonging to the Rosaceae family. Almond has been used as base material in pharmaceutical, cosmetic, hygienically and food industry. Propagation by tissue culture technique is the most important one in woody plants. In the current research, in vitro optimization of tissue culture and mass production of almond was investigated. In this idea, explants of actively growing shoots were collected and sterilized, then transferred to MS medium with different concentrations and combinations of plant growth regulators. The experiment was done in completely randomized blocks design, with 7 treatment and 30 replications. After 4 weeks, calli induction, proliferation, shoot length and number of shoot per explants were measured. Results showed that the best medium for shoot initiation and proliferation was MS + 0.5 mg/l IAA (Indol-3-Acetic Acid) + 1 mg/l BA (Benzyl Adenine). Autumn was the best season for collecting explants. The shoots were transferred to root induction medium with different concentrations of plant growth regulators. The best root induction medium was MS + 0.5 mg/l IBA (Indol Butyric Acid).

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Priming and storage of amaranth seeds: effects of plant growth regulators on germination performance at low temperature

Seed Science and Technology ◽

10.15258/sst.2006.34.1.18 ◽

2006 ◽

Vol 34 (1) ◽

pp. 169-179 ◽

Cited By ~ 2

Author(s):

I. Tiryaki

Keyword(s):

Low Temperature ◽

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

And Storage

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Phytohormones Influence on the In-Vitro Regeneration of Saccharum Officinarum L. from Apical Meristem Culture

Journal of Biology and Life Science ◽

10.5296/jbls.v5i2.4885 ◽

2014 ◽

Vol 5 (2) ◽

pp. 85 ◽

Cited By ~ 1

Author(s):

Ejiroghene Felix Lawyer ◽

Z. O. Jamaleddine ◽

P. T. Lyam ◽

I. T. Borokini ◽

A. A. Adedeji ◽

...

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Apical Meristem ◽

In Vitro Regeneration ◽

Saccharum Officinarum ◽

Naphthalene Acetic Acid ◽

Root Induction ◽

Indole Butyric Acid

Growth regulators especially auxins and cytokinins are critical for plant in-vitro regeneration. The effect of these plant growth regulators on in-vitro propagation of Saccharum officinarum L (Sugarcane) was investigated. In vitro response of two different varieties of sugarcane (NCS 005 and NCS 008) to Plant Growth Regulators was obtained in this study. Formation of buds was obtained on shoot apical meristem when cultured on MS (Murashige and Skoog) medium supplemented with 0.1mg/l BAP (6-Benzylaminopurine). After two weeks of initiation, regenerated meristem was inoculated into MS (Murashige and Skoog) fortified with different concentrations and combination of cytokinins. Shoot multiplication was optimal on 0.5mg/l BAP + 0.25 mg/l Kin(Kinetin) for NCS 005 variety while for NCS 008 variety, no significant (P≥0.05) difference was observed between 1.5mg/l BAP and 1.5mg/l BAP +0.5mg/l Kin. The best root induction for in vitro derived shoots was obtained on 1.0 mg/l NAA (Naphthalene acetic acid) and 2.0 mg/l IBA( Indole butyric acid) for both varieties of sugarcane within ten days of culture transfer. Successfully established plantlets showed excellent growth response when weaned under regulated green house conditions.

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Micropropagation of cloudberry (Rubus chamaemorus L.) by initiation of axillary shoots

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.2001.002 ◽

2014 ◽

Vol 70 (1) ◽

pp. 11-16 ◽

Cited By ~ 5

Author(s):

Barbara Thiem

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Botanical Garden ◽

Axillary Shoot ◽

Root Induction ◽

Ms Medium ◽

Axillary Shoots ◽

Rubus Chamaemorus ◽

Step Procedure

A method for micropropagation of the endangered <em>Rubus chamaemorus</em> L. (<em>Rosaceae</em>) from shoot tips was developed. Murashige and Skoog (MS) medium supplemented with BA and IBA in different concentrations was used for axillary shoot development. The most effective was MS medium containing 0.88 µM BA and 0.49 µM IBA with value of pH = 4.0. Cultures of microshoots (up to 12 shoots from a single explant) developed into regular shoots on the same medium or after transferring them onto MS media with 0.44 µM BA, 0.49 µM IBA and 434 µM SA. The rooting of initiated axillary shoots proved extremely difficult. A two-step procedure for root induction was adopted. Auxin- treated shoots were put to medium devoid of plant growth regulators. Rooted shoots were then transferred to pots and grown in the greenhouse until planted in the ground of the Botanical Garden.

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