Isolation and characterization of 14 tetranucleotide microsatellite loci for the cannonball jellyfish (Stomolophus sp.) by next generation sequencing

2017 ◽  
Vol 44 (2) ◽  
pp. 257-260
Author(s):  
Leandro Nicolás Getino-Mamet ◽  
Tania Valdivia-Carrillo ◽  
Liza Gómez Daglio ◽  
Francisco Javier García-De León
Keyword(s):  
Next Generation Sequencing ◽  
Microsatellite Loci ◽  
Next Generation ◽  
Isolation And Characterization ◽  
Generation Sequencing

scholarly journals Isolation and characterization of 23 microsatellite loci in the stingless bee Melipona subnitida using next-generation sequencing

2014 ◽  
Vol 7 (1) ◽  
pp. 239-241 ◽  
Author(s):  
Isis G. B. Souza ◽  
Ian Paterson ◽  
Meghan C. McBride ◽  
Bruno A. Souza ◽  
Fabia M. Pereira ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Microsatellite Loci ◽  
Stingless Bee ◽  
Next Generation ◽  
Melipona Subnitida ◽  
Isolation And Characterization ◽  
Generation Sequencing

scholarly journals Next Generation Sequencing, Isolation and Characterization of 14 Microsatellite Loci of Canthon Cyanellus (Coleoptera: Scarabaeidae)

2021 ◽  
Author(s):  
Luis R. Arce-Valdés ◽  
Rosa Ana Sánchez-Guillén ◽  
Janet Nolasco-Soto ◽  
Mario E. Favila
Keyword(s):  
Next Generation Sequencing ◽  
Microsatellite Loci ◽  
Dung Beetle ◽  
Distribution Range ◽  
Next Generation ◽  
Isolation And Characterization ◽  
Paired End Sequencing ◽  
Generation Sequencing

Abstract BackgroundWe used Illumina paired-end-sequencing to isolate and characterize microsatellites of Canthon cyanellus, a Neotropical roller dung beetle encompassing several lineages within its distribution range.Methods and ResultsWe examined C. cyanellus specimens collected at eight different localities in Mexico (two or three specimens per locality). We initially made amplification tests with 16 loci, but two of these showed amplification problems; thus, we evaluated 14 loci. The 14 microsatellites were polymorphic, with 2–16 alleles each. The expected and observed heterozygosity ranged from 0.11 to 0.76 and from 0.20 to 0.78, respectively. ConclusionsThese microsatellites will help to assess structure at the population and lineage levels, identify zones of potential hybridization between lineages, and draw a more precise geographic delimitation of Canthon cyanellus lineages.

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