scholarly journals Isolation and characterization of 38 SNP markers for the black rockfish, Sebastes schlegelii by next-generation sequencing

2021 ◽  
Author(s):  
Xianggang Gao ◽  
Xiangbo Bao ◽  
Wei Sun ◽  
Yunfeng Li ◽  
Zongying Liu ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Snp Markers ◽  
Next Generation ◽  
Black Rockfish ◽  
Isolation And Characterization ◽  
Sebastes Schlegelii ◽  
Generation Sequencing

scholarly journals Next Generation Sequencing, Isolation and Characterization of 14 Microsatellite Loci of Canthon Cyanellus (Coleoptera: Scarabaeidae)

2021 ◽  
Author(s):  
Luis R. Arce-Valdés ◽  
Rosa Ana Sánchez-Guillén ◽  
Janet Nolasco-Soto ◽  
Mario E. Favila
Keyword(s):  
Next Generation Sequencing ◽  
Microsatellite Loci ◽  
Dung Beetle ◽  
Distribution Range ◽  
Next Generation ◽  
Isolation And Characterization ◽  
Paired End Sequencing ◽  
Generation Sequencing

Abstract BackgroundWe used Illumina paired-end-sequencing to isolate and characterize microsatellites of Canthon cyanellus, a Neotropical roller dung beetle encompassing several lineages within its distribution range.Methods and ResultsWe examined C. cyanellus specimens collected at eight different localities in Mexico (two or three specimens per locality). We initially made amplification tests with 16 loci, but two of these showed amplification problems; thus, we evaluated 14 loci. The 14 microsatellites were polymorphic, with 2–16 alleles each. The expected and observed heterozygosity ranged from 0.11 to 0.76 and from 0.20 to 0.78, respectively. ConclusionsThese microsatellites will help to assess structure at the population and lineage levels, identify zones of potential hybridization between lineages, and draw a more precise geographic delimitation of Canthon cyanellus lineages.

scholarly journals Isolation And Characterization Of 38 SNP Markers For The Black Rockfish, Sebastes Schlegelii By Next-Generation Sequencing

2021 ◽  
Author(s):  
Xianggang Gao ◽  
Xiangbo Bao ◽  
Wei Sun ◽  
Yunfeng Li ◽  
Zongying Liu ◽  
...  
Keyword(s):  
Polymorphic Information Content ◽  
Snp Markers ◽  
Habitat Destruction ◽  
Single Nucleotide ◽  
Genetic Studies ◽  
Important Species ◽  
Black Rockfish ◽  
Isolation And Characterization ◽  
Sebastes Schlegelii

Abstract The black rockfish (Sebastes schlegelii) is an economically important species. However, wild S. schlegelii resources have declined sharply in recent years as a result of human disturbance and habitat destruction. Thus, it is crucial to protect the current resources of S. schlegelii. In this study, 38 novel single nucleotide polymorphism (SNP) markers were developed based on restriction-site associated DNA sequencing. The results showed that the observed heterozygosity and expected heterozygosity ranged from 0.1400 to 0.6400 and 0.1487 to 0.4978, respectively. The minor allele frequency raged from 0.1429 to 0.4694. Polymorphic information content ranged from 0.174 to 0.365. Four SNPs were found to be deviated significantly from the HWE (P<0.05). These SNP markers will serve as a useful tool for genetic studies and population evaluation aimed at the conservation of S. schlegelii.

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