Sensitivity of Intracellular Calcium-Binding Sites for Exo- and Endocytosis of Synaptic Vesicles to Sr, Ba, and Mg Ions

2010 ◽  
Vol 40 (4) ◽  
pp. 389-396 ◽  
Author(s):  
A. L. Zefirov ◽  
P. N. Grigor’ev
Keyword(s):  
Intracellular Calcium ◽  
Binding Sites ◽  
Calcium Binding ◽  
Synaptic Vesicles ◽  
Calcium Binding Sites

Ultrastructural localization of calcium-binding sites in the electrocyte of Electrophorus electricus (L.)

1979 ◽  
Vol 38 (1) ◽  
pp. 97-104
Author(s):  
T.C. De Araujo Jorge ◽  
W. De Souza ◽  
R.D. Machado
Keyword(s):  
Plasma Membrane ◽  
Binding Sites ◽  
Calcium Binding ◽  
Synaptic Vesicles ◽  
Ultrastructural Localization ◽  
Synaptic Membrane ◽  
Electrophorus Electricus ◽  
Calcium Binding Sites

Calcium-binding sites were detected in the electrocyte of Electrophorus electricus (L.) using the Oschman & Wall technique, in which CaCl2 was added to the fixative and washing solutions. Deposits were seen scattered along the plasma membrane of the electrocyte, inside mitochondria, associated with the post-synaptic membrane and the membrane of synaptic vesicles.

scholarly journals Intracellular Calcium Binding and Release in Frog Heart

1973 ◽  
Vol 62 (6) ◽  
pp. 693-706 ◽  
Author(s):  
Saul Winegrad
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Intracellular Calcium ◽  
Binding Sites ◽  
Calcium Binding ◽  
Calcium Content ◽  
Frog Heart ◽  
Affinity Constant ◽  
Total Calcium ◽  
Abrupt Changes ◽  
Calcium Binding Sites

The capacities and affinities of intracellular calcium-binding sites have been studied in frog ventricles, in which the concentration of Ca++ in the sarcoplasm can be controlled as a result of treatment with EDTA. The total calcium content of calcium-depleted and nondepleted muscles at rest and muscles generating considerable tension was 0.8, 1.4, and 5.4 µmol/g of muscle, respectively. Net movement of calcium into or out of the cells occurred without change in tension when the sarcoplasmic concentration of Ca++ was either of two values, less than 10-7 M or approximately 5 x 10-7 M. These data can be explained by the presence of two groups of intracellular calcium sinks which compete with the contractile proteins, one with a capacity of about 0.6 µmol/g and an affinity constant greater than 107 M-1 and a second with a capacity of 4.0 µmol/g and an affinity constant of about 2 x 106 M-1. The higher affinity calcium is released by anoxia, oligomycin, or abrupt changes in sarcoplasmic Ca++. Muscles soaked in Sr-Ringer's contain electron densities in the sarcoplasmic reticulum and to a lesser extent in the mitochondria.

scholarly journals THE CALCIUM BINDING SITES OF SYNAPTIC VESICLES OF THE FROG SARTORIUS NEUROMUSCULAR JUNCTION

1974 ◽  
Vol 61 (3) ◽  
pp. 818-823 ◽  
Author(s):  
Alberto L. Politoff ◽  
Steven Rose ◽  
George D. Pappas
Keyword(s):  
Neuromuscular Junction ◽  
Binding Sites ◽  
Calcium Binding ◽  
Synaptic Vesicles ◽  
Calcium Binding Sites ◽  
Frog Sartorius

scholarly journals Mutation of the high affinity calcium binding sites in cardiac troponin C.

1992 ◽  
Vol 267 (2) ◽  
pp. 825-831 ◽  
Author(s):  
J C Negele ◽  
D G Dotson ◽  
W Liu ◽  
H L Sweeney ◽  
J A Putkey
Keyword(s):  
Binding Sites ◽  
Calcium Binding ◽  
Cardiac Troponin ◽  
Troponin C ◽  
High Affinity ◽  
Cardiac Troponin C ◽  
Calcium Binding Sites

Rat GTP cyclohydrolase I is a homodecameric protein complex containing high-affinity calcium-binding sites 1 1Edited by W. Baumeister

1998 ◽  
Vol 279 (1) ◽  
pp. 189-199 ◽  
Author(s):  
Michel O Steinmetz ◽  
Christoph Plüss ◽  
Urs Christen ◽  
Bettina Wolpensinger ◽  
Ariel Lustig ◽  
...  
Keyword(s):  
Protein Complex ◽  
Binding Sites ◽  
Calcium Binding ◽  
Gtp Cyclohydrolase I ◽  
Gtp Cyclohydrolase ◽  
High Affinity ◽  
Calcium Binding Sites

Visualization of calcium-binding sites at plasma membrane of shock-frozen Acanthamoeba cells

1981 ◽  
Vol 68 (1) ◽  
pp. 125-129 ◽  
Author(s):  
Andrzej Sobota ◽  
Aleksandra Przelecka
Keyword(s):  
Plasma Membrane ◽  
Binding Sites ◽  
Calcium Binding ◽  
Calcium Binding Sites

scholarly journals Characterization of the calcium-binding sites of calcineurin B

FEBS Letters ◽  
1995 ◽  
Vol 362 (1) ◽  
pp. 55-58 ◽  
Author(s):  
Lazaros T Kakalis ◽  
Michael Kennedy ◽  
Robert Sikkink ◽  
Frank Rusnak ◽  
Ian M Armitage
Keyword(s):  
Binding Sites ◽  
Calcium Binding ◽  
Calcineurin B ◽  
Calcium Binding Sites
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