Treatment In vitro of Retinal Cells with IL-4 Increases the Survival of Retinal Ganglion Cells: The Involvement of BDNF

2012 ◽  
Vol 38 (1) ◽  
pp. 162-173 ◽  
Author(s):  
Leandro de Araujo-Martins ◽  
Raphael Monteiro de Oliveira ◽  
Gabriela Velozo Gomes dos Santos ◽  
Renata Cláudia Celestino dos Santos ◽  
Aline Araujo dos Santos ◽  
...  
Keyword(s):  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
Retinal Ganglion ◽  
Retinal Cells

scholarly journals A Safe GDNF and GDNF/BDNF Controlled Delivery System Improves Migration in Human Retinal Pigment Epithelial Cells and Survival in Retinal Ganglion Cells: Potential Usefulness in Degenerative Retinal Pathologies

2021 ◽  
Vol 14 (1) ◽  
pp. 50
Author(s):  
Alicia Arranz-Romera ◽  
Maria Hernandez ◽  
Patricia Checa-Casalengua ◽  
Alfredo Garcia-Layana ◽  
Irene T. Molina-Martinez ◽  
...  
Keyword(s):  
Cell Viability ◽  
Retinal Ganglion Cells ◽  
Neurotrophic Factor ◽  
Retinal Pigment Epithelial ◽  
Ganglion Cells ◽  
Retinal Pigment ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Retinal Ganglion ◽  
Pigment Epithelial

We assessed the sustained delivery effect of poly (lactic-co-glycolic) acid (PLGA)/vitamin E (VitE) microspheres (MSs) loaded with glial cell-derived neurotrophic factor (GDNF) alone (GDNF-MSs) or combined with brain-derived neurotrophic factor (BDNF; GDNF/BDNF-MSs) on migration of the human adult retinal pigment epithelial cell-line-19 (ARPE-19) cells, primate choroidal endothelial (RF/6A) cells, and the survival of isolated mouse retinal ganglion cells (RGCs). The morphology of the MSs, particle size, and encapsulation efficiencies of the active substances were evaluated. In vitro release, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell viability, terminal deoxynucleotidyl transferase (TdT) deoxyuridine dUTP nick-end labelling (TUNEL) apoptosis, functional wound healing migration (ARPE-19; migration), and (RF/6A; angiogenesis) assays were conducted. The safety of MS intravitreal injection was assessed using hematoxylin and eosin, neuronal nuclei (NeuN) immunolabeling, and TUNEL assays, and RGC in vitro survival was analyzed. MSs delivered GDNF and co-delivered GDNF/BDNF in a sustained manner over 77 days. The BDNF/GDNF combination increased RPE cell migration, whereas no effect was observed on RF/6A. MSs did not alter cell viability, apoptosis was absent in vitro, and RGCs survived in vitro for seven weeks. In mice, retinal toxicity and apoptosis was absent in histologic sections. This delivery strategy could be useful as a potential co-therapy in retinal degenerations and glaucoma, in line with future personalized long-term intravitreal treatment as different amounts (doses) of microparticles can be administered according to patients’ needs.

Glucocorticoid effects on axonal regeneration of retinal ganglion cells in vitro

1994 ◽  
Vol 6 (2) ◽  
pp. 83-87
Author(s):  
Barbara Szymankiewicz-Rak ◽  
Jörg Mey ◽  
Solon Thanos
Keyword(s):  
Retinal Ganglion Cells ◽  
Axonal Regeneration ◽  
Ganglion Cells ◽  
Retinal Ganglion

scholarly journals Altered Transport Velocity of Axonal Mitochondria in Retinal Ganglion Cells After Laser-Induced Axonal Injury In Vitro

2015 ◽  
Vol 56 (13) ◽  
pp. 8019 ◽  
Author(s):  
Satoshi Yokota ◽  
Yuji Takihara ◽  
Shogo Arimura ◽  
Seiji Miyake ◽  
Yoshihiro Takamura ◽  
...  
Keyword(s):  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
Axonal Injury ◽  
Retinal Ganglion ◽  
Transport Velocity ◽  
Altered Transport

Tafluprost protects rat retinal ganglion cells from apoptosis in vitro and in vivo

2009 ◽  
Vol 247 (10) ◽  
pp. 1353-1360 ◽  
Author(s):  
Akiyasu Kanamori ◽  
Maiko Naka ◽  
Masahide Fukuda ◽  
Makoto Nakamura ◽  
Akira Negi
Keyword(s):  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
Retinal Ganglion

miR-223 induces retinal ganglion cells apoptosis and inflammation via decreasing HSP-70 in vitro and in vivo

2020 ◽  
Vol 104 ◽  
pp. 101747 ◽  
Author(s):  
Yun Ou-Yang ◽  
Zheng-Li Liu ◽  
Chun-Long Xu ◽  
Jia-Liang Wu ◽  
Jun Peng ◽  
...  
Keyword(s):  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
Retinal Ganglion ◽  
Hsp 70 ◽  
Apoptosis And Inflammation
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