Seasonal effect on tissue culture response and plant regeneration frequency from non-bombarded and bombarded immature scutella of barley (Hordeum vulgare) harvested from controlled environment

2005 ◽  
Vol 81 (1) ◽  
pp. 19-26 ◽  
Author(s):  
Vijendra K. Sharma ◽  
Robert H�nsch ◽  
Ralf R. Mendel ◽  
Jutta Schulze
Keyword(s):  
Tissue Culture ◽  
Hordeum Vulgare ◽  
Plant Regeneration ◽  
Seasonal Effect ◽  
Controlled Environment ◽  
Regeneration Frequency ◽  
Tissue Culture Response

Tissue culture response of Beta germplasm: callus induction and plant regeneration

1991 ◽  
Vol 27 (2) ◽  
pp. 123-127 ◽  
Author(s):  
J. Abe ◽  
H. Nakashima ◽  
K. Mitsui ◽  
T. Mikami ◽  
Y. Shimamoto
Keyword(s):  
Tissue Culture ◽  
Plant Regeneration ◽  
Callus Induction ◽  
Tissue Culture Response

Evaluation of 10 Canadian barley (Hordeum vulgare L.) cultivars for tissue culture response

1993 ◽  
Vol 73 (1) ◽  
pp. 171-174 ◽  
Author(s):  
A. M. R. Baillie ◽  
K. K. Kartha ◽  
B. G. Rossnagel
Keyword(s):  
Tissue Culture ◽  
Hordeum Vulgare ◽  
Growth Regulators ◽  
Dichlorophenoxyacetic Acid ◽  
Hordeum Vulgare L ◽  
Tissue Culture Response ◽  
Barley Cultivars ◽  
Best Response ◽  
Regenerated Plants ◽  
2,4 Dichlorophenoxyacetic Acid

Ten Canadian barley cultivars — Abee, Deuce, Ellice, Harrington, Manley, Bonanza, Conquest, Duke, Heartland, and Samson — were evaluated for tissue-culture response. Callus was obtained from embryos 3–5 d post anthesis from all cultivars. Fertile plants were regenerated from eight. Abee cultures gave the best response in terms of the number of plants regenerated, while Bonanza and Samson cultures produced no regenerated plants. Heartland and Deuce were selected for further study to determine optimum growth-regulator concentrations for callus production and plant regeneration. Two growth regulators — 2,4-dichlorophenoxyacetic acid (2,4-D) and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) — were evaluated at five concentrations (0.5, 1.0, 2.5, 5.0 and 10 mg L−1). Maximum regeneration rates were achieved with Gamborg’s B5 medium supplemented with 2.5 mg L−1 2,4-D. Thirty-four Heartland and 19 Deuce regenerants were produced per 100 embryos cultured. Key words: Barley, growth regulators, Hordeum vulgare, regeneration, tissue culture

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