scholarly journals Metagenomic sequencing determines complete infectious bronchitis virus (avian Gammacoronavirus) vaccine strain genomes and associated viromes in chicken clinical samples

Virus Genes ◽  
2021 ◽  
Author(s):  
Steven Van Borm ◽  
Mieke Steensels ◽  
Elisabeth Mathijs ◽  
Frank Vandenbussche ◽  
Thierry van den Berg ◽  
...  
Keyword(s):  
Vaccine Strain ◽  
Infectious Bronchitis Virus ◽  
Clinical Samples ◽  
Metagenomic Sequencing ◽  
Infectious Bronchitis

Transmissibility of Infectious Bronchitis Virus H120 Vaccine Strain Among Broilers Under Experimental Conditions

2008 ◽  
Vol 52 (3) ◽  
pp. 461-466 ◽  
Author(s):  
M. G. R. Matthijs ◽  
A. Bouma ◽  
F. C. Velkers ◽  
J. H. H. van Eck ◽  
J. A. Stegeman
Keyword(s):  
Vaccine Strain ◽  
Infectious Bronchitis Virus ◽  
Experimental Conditions ◽  
Infectious Bronchitis

scholarly journals Development of an attenuated vaccine strain from a korean respiratory type infectious bronchitis virus

2011 ◽  
Vol 51 (3) ◽  
pp. 193-201
Author(s):  
Kang-Seuk Choi ◽  
Woo-Jin Jeon ◽  
Eun-Kyoung Lee ◽  
Soo-Jeong Kye ◽  
Mi-Ja Park ◽  
...  
Keyword(s):  
Vaccine Strain ◽  
Infectious Bronchitis Virus ◽  
Infectious Bronchitis ◽  
Attenuated Vaccine

scholarly journals Construction of an infectious bronchitis virus vaccine strain carrying chimeric S1 gene of a virulent isolate and its pathogenicity analysis

2020 ◽  
Vol 104 (19) ◽  
pp. 8427-8437
Author(s):  
Chenfei Lv ◽  
Tingting Shi ◽  
Pengpeng Zhu ◽  
Xing Peng ◽  
Shangshang Cao ◽  
...  
Keyword(s):  
Vaccine Strain ◽  
Virus Vaccine ◽  
Infectious Bronchitis Virus ◽  
S1 Gene ◽  
Infectious Bronchitis ◽  
Virulent Isolate

scholarly journals Delmarva (DMV/1639) Infectious Bronchitis Virus (IBV) Variants Isolated in Eastern Canada Show Evidence of Recombination

Viruses ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 1054 ◽  
Author(s):  
Mohamed S. H. Hassan ◽  
Davor Ojkic ◽  
Carla S. Coffin ◽  
Susan C. Cork ◽  
Frank van der Meer ◽  
...  
Keyword(s):  
Infectious Bronchitis Virus ◽  
Genomic Sequence ◽  
Sequence Similarity ◽  
Clinical Samples ◽  
Complete Genomic Sequence ◽  
Eastern Canada ◽  
Diagnostic Laboratory ◽  
Infectious Bronchitis ◽  
Show Evidence ◽  
Complete Genomic

Infectious bronchitis virus (IBV) infection in chickens can lead to an economically important disease, namely, infectious bronchitis (IB). New IBV variants are continuously emerging, which complicates vaccination-based IB control. In this study, five IBVs were isolated from clinical samples submitted to a diagnostic laboratory in Ontario, Canada, and subjected to detailed molecular characterization. Analysis of the spike (S)1 gene showed that these five IBVs were highly related to the Delmarva (DMV/1639) strain (~97.0% nucleotide sequence similarity) that was firstly isolated from an IB outbreak in the Delmarva peninsula, United States of America (USA), in 2011. However, the complete genomic sequence analysis showed a 93.5–93.7% similarity with the Connecticut (Conn) vaccine strain, suggesting that Conn-like viruses contributed to the evolution of the five Canadian IBV/DMV isolates. A SimPlot analysis of the complete genomic sequence showed evidence of recombination for at least three different IBV strains, including a Conn vaccine-like strain, a 4/91 vaccine-like strain, and one strain that is yet-unidentified. The unidentified strain may have contributed the genomic regions of the S, 3, and membrane (M) genes of the five Canadian IBV/DMV isolates. The study outcomes add to the existing knowledge about involvement of recombination in IBV evolution.

Transmissibility of Infectious Bronchitis Virus H120 Vaccine Strain among Broilers under Experimental Conditions

2008 ◽  
Vol 3 (3) ◽  
pp. e16-e16
Author(s):  
M. G. R. Matthijs ◽  
A. Bouma ◽  
F. C. Velkers ◽  
J. H. H. van Eck ◽  
J. A. Stegeman
Keyword(s):  
Vaccine Strain ◽  
Infectious Bronchitis Virus ◽  
Experimental Conditions ◽  
Infectious Bronchitis

scholarly journals Complete genome sequence and recombination analysis of infectious bronchitis virus attenuated vaccine strain H120

Virus Genes ◽  
2010 ◽  
Vol 41 (3) ◽  
pp. 377-388 ◽  
Author(s):  
Yi Zhang ◽  
Hong-Ning Wang ◽  
Ting Wang ◽  
Wen-Qiao Fan ◽  
An-Yun Zhang ◽  
...  
Keyword(s):  
Vaccine Strain ◽  
Genome Sequence ◽  
Infectious Bronchitis Virus ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Recombination Analysis ◽  
Infectious Bronchitis ◽  
Attenuated Vaccine

scholarly journals Molecular epizootiology of infectious bronchitis virus in Sweden indicating the involvement of a vaccine strain

2002 ◽  
Vol 31 (3) ◽  
pp. 229-236 ◽  
Author(s):  
A. Farsang ◽  
C. Ros ◽  
Lena H. M. Renström ◽  
Claudia Baule ◽  
T. Soós ◽  
...  
Keyword(s):  
Vaccine Strain ◽  
Infectious Bronchitis Virus ◽  
Infectious Bronchitis

scholarly journals Real-time, MinION-based, amplicon sequencing for lineage typing of infectious bronchitis virus from upper respiratory samples

2020 ◽  
pp. 104063872091010
Author(s):  
Salman L. Butt ◽  
Eric C. Erwood ◽  
Jian Zhang ◽  
Holly S. Sellers ◽  
Kelsey Young ◽  
...  
Keyword(s):  
Infectious Bronchitis Virus ◽  
Amplicon Sequencing ◽  
Economic Losses ◽  
Clinical Samples ◽  
Strain Identification ◽  
Accurate Identification ◽  
Oral Swab ◽  
Spike Gene ◽  
Infectious Bronchitis ◽  
Sequencing Library

Infectious bronchitis (IB) causes significant economic losses in the global poultry industry. Control of IB is hindered by the genetic diversity of the causative agent, infectious bronchitis virus (IBV), which has led to the emergence of several serotypes that lack complete serologic cross-protection. Although serotyping requires immunologic characterization, genotyping is an efficient means to identify IBVs detected in samples. Sanger sequencing of the S1 subunit of the spike gene is currently used to genotype IBV; however, the universal S1 PCR was created to work from cultured IBV, and it is inefficient at detecting multiple viruses in a single sample. We describe herein a MinION-based, amplicon-based sequencing (AmpSeq) method that genetically categorized IBV from clinical samples, including samples with multiple IBVs. Total RNA was extracted from 15 tracheal scrapings and choanal cleft swab samples, randomly reverse transcribed, and PCR amplified using modified S1-targeted primers. Amplicons were barcoded to allow for pooling of samples, processed per manufacturer’s instructions into a 1D MinION sequencing library, and then sequenced on the MinION. The AmpSeq method detected IBV in 13 of 14 IBV-positive samples. AmpSeq accurately detected and genotyped both IBV lineages in 3 of 5 samples containing 2 IBV lineages. Additionally, 1 sample contained 3 IBV lineages, and AmpSeq accurately detected 2 of the 3 lineages. Strain identification, including detection of different IBVs from the same lineage, was also possible with this AmpSeq method. Our results demonstrate the feasibility of using MinION-based AmpSeq for rapid and accurate identification and lineage typing of IBV from oral swab samples.

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