Investigation of diethylstilbestrol residue level in human urine samples by a specific monoclonal antibody

2017 ◽  
Vol 24 (8) ◽  
pp. 7042-7050 ◽  
Author(s):  
Dai Zhang ◽  
Lifang Zhou ◽  
Yajing Lei ◽  
Zhan Zhou ◽  
Jie Zhou ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Human Urine ◽  
Residue Level ◽  
Urine Samples ◽  
Specific Monoclonal Antibody ◽  
Human Urine Samples

scholarly journals Competitive enzyme immunoassay with monoclonal antibody for homovanillic acid measurement in human urine samples

1997 ◽  
Vol 43 (2) ◽  
pp. 363-368 ◽  
Author(s):  
Frédéric Taran ◽  
Yveline Frobert ◽  
Christophe Créminon ◽  
Jacques Grassi ◽  
Didier Olichon ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Enzyme Immunoassay ◽  
Human Urine ◽  
Homovanillic Acid ◽  
Colorimetric Assay ◽  
Cross Reactivity ◽  
Urine Samples ◽  
Vanillylmandelic Acid ◽  
Human Urine Samples ◽  
Enzyme Detection

Abstract A fast competitive enzyme immunoassay (EIA) for measuring homovanillic acid in human urine samples was developed with a monoclonal antibody and acetylcholinesterase as enzyme label. Enzyme detection was performed by an easy colorimetric assay. Monoclonal antibodies were screened on the basis of sensitivity, specificity, and correlation studies. EIA has a detection limit of 0.5 μmol/L, a CV <10% in the 1.25–10 μmol/L range, and intra- and interassay CVs of <10%. Cross-reactivity with vanillylmandelic acid was 0.5% and <8% for other structurally related catecholamine metabolites. Parallelism of the EIA was shown in dilution studies and the correlation with routine HPLC assay in 62 normal and pathologic samples was EIA = 1.492 (HPLC) − 3.46, Sy|x = 47.52, range = 4–1800 μmol/L, r2 = 0.977. Additional data concerning the validity of this assay were provided by HPLC analysis of urinary immunoreactive material.

scholarly journals Stanozolol‐ N ‐glucuronide metabolites in human urine samples as suitable targets in terms of routine anti‐doping analysis

2021 ◽  
Author(s):  
Lorenz Göschl ◽  
Günter Gmeiner ◽  
Peter Gärtner ◽  
Georg Stadler ◽  
Valentin Enev ◽  
...  
Keyword(s):  
Human Urine ◽  
Urine Samples ◽  
Doping Analysis ◽  
Human Urine Samples

scholarly journals The colorimetric and microfluidic paper-based detection of cysteine and homocysteine using 1,5-diphenylcarbazide-capped silver nanoparticles

RSC Advances ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 3295-3303 ◽  
Author(s):  
Sattar Shariati ◽  
Gholamreza Khayatian
Keyword(s):  
Silver Nanoparticles ◽  
Human Urine ◽  
Quantitative Measurement ◽  
Urine Samples ◽  
Human Urine Samples

A simple and novel portable method for the quantitative measurement of cysteine and homocysteine in human urine samples is presented.

scholarly journals Analytical and physiological validation of an enzyme immunoassay to measure oxytocin in dog, wolf, and human urine samples

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
G. Wirobski ◽  
F. S. Schaebs ◽  
F. Range ◽  
S. Marshall-Pescini ◽  
T. Deschner
Keyword(s):  
Enzyme Immunoassay ◽  
Human Urine ◽  
Extraction Efficiency ◽  
Urine Samples ◽  
Antibody Specificity ◽  
Analytical Validation ◽  
Human Urine Samples ◽  
Assay Performance ◽  
Fit For Purpose ◽  
Commercial Enzyme Immunoassay

AbstractOxytocin (OT) promotes pro-sociality, bonding, and cooperation in a variety of species. Measuring oxytocin metabolite (OTM) concentrations in urine or saliva provides intriguing opportunities to study human and animal behaviour with minimal disturbance. However, a thorough validation of analytical methods and an assessment of the physiological significance of these measures are essential. We conducted an analytical validation of a commercial Enzyme Immunoassay (EIA; Arbor OT assay kit) to measure OTM concentrations in dog, wolf, and human urine samples. To test the assay’s ability to detect changes in OTM concentrations, we administered oxytocin intranasally to 14 dogs. Assay performance with regard to parallelism was acceptable. Assay accuracy and extraction efficiency for dog and wolf samples were comparable to a previously validated assay (Enzo OT assay kit) but variation was smaller for human samples. Binding sensitivity and antibody specificity were better in the Arbor assay. Average OTM concentrations were more than twice as high as in comparable samples measured with the Enzo assay, highlighting a lack of comparability of absolute values between different assays. Changes in OTM concentrations after intranasal treatment were detected reliably. The Arbor assay met requirements of a “fit-for-purpose” validation with improvement of several parameters compared to the Enzo assay.

CE-ESI-MS coupled with dynamic pH junction online concentration for analysis of peptides in human urine samples

2010 ◽  
Vol 31 (20) ◽  
pp. 3400-3406 ◽  
Author(s):  
Hongzhi Ye ◽  
Shifei Xia ◽  
Wei Lin ◽  
Lishuang Yu ◽  
Xueqin Xu ◽  
...  
Keyword(s):  
Human Urine ◽  
Urine Samples ◽  
Human Urine Samples ◽  
Esi Ms ◽  
Dynamic Ph Junction
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