Development of a serum-free medium for dihydrofolate reductase-deficient chinese hamster ovary cells (DG44) using a statistical design: Beneficial effect of weaning of cells

1999 ◽  
Vol 35 (4) ◽  
pp. 178-182 ◽  
Author(s):  
Eun Jung Kim ◽  
No Soo Kim ◽  
Gyun Min Lee
Keyword(s):  
Beneficial Effect ◽  
Dihydrofolate Reductase ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Statistical Design ◽  
Serum Free Medium ◽  
Free Medium ◽  
Ovary Cells ◽  
Serum Free

scholarly journals Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells

2021 ◽  
Vol 9 ◽  
Author(s):  
Weifeng Li ◽  
Zhenlin Fan ◽  
Yan Lin ◽  
Tian-Yun Wang
Keyword(s):  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Recombinant Protein Expression ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Serum Free Medium ◽  
Free Medium ◽  
Ovary Cells ◽  
Serum Free ◽  
Small Molecule Compound

At present, nearly 70% of recombinant therapeutic proteins (RTPs) are produced by Chinese hamster ovary (CHO) cells, and serum-free medium (SFM) is necessary for their culture to produce RTPs. In this review, the history and key components of SFM are first summarized, and its preparation and experimental design are described. Some small molecule compound additives can improve the yield and quality of RTP. The function and possible mechanisms of these additives are also reviewed here. Finally, the future perspectives of SFM use with CHO cells for RTP production are discussed.

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