An Improved Micropropagation of Arnebia hispidissima (Lehm.) DC. and Assessment of Genetic Fidelity of Micropropagated Plants Using DNA-Based Molecular Markers

2013 ◽  
Vol 170 (5) ◽  
pp. 1163-1173 ◽  
Author(s):  
Mahendra Phulwaria ◽  
Manoj K. Rai ◽  
N. S. Shekhawat
Keyword(s):  
Molecular Markers ◽  
Genetic Fidelity ◽  
Arnebia Hispidissima ◽  
Micropropagated Plants

scholarly journals ASSESSMENT OF GENETIC STABILITY OF MICROPROPAGATED Eucalyptus globulus Labill HYBRID CLONES BY MEANS OF FLOW CYTOMETRY AND MICROSATELLITES MARKERS

2017 ◽  
Vol 41 (1) ◽  
Author(s):  
Leandro Silva Oliveira ◽  
Aloisio Xavier ◽  
Wagner Campos Otoni ◽  
José Marcello Salabert Campos ◽  
Lyderson Facio Viccini ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Microsatellite Markers ◽  
Genetic Stability ◽  
Culture Medium ◽  
Eucalyptus Grandis ◽  
Genetic Fidelity ◽  
Shoot Multiplication ◽  
Micropropagated Plants ◽  
Hybrid Clones

ABSTRACT Flow cytometry and microsatellite markers were used to determine a genetic fidelity of micropropagated plants from the two Eucalyptus urophylla x E. globulus clones and a Eucalyptus grandis x E. globulus clone derived from adult material. Clones were repeatedly subcultured for 25 subcultures on MS medium supplemented with BA (2.22 µM) and ANA (0.05 µM) for in vitro shoot multiplication. The elongation was performed in MS culture medium supplemented with AIB (2.46 µM) and BA(0.22 µM). The ex vitro rooting and acclimatization phases were lead at the same time. The micropropagated clones showed genetic stability by flow cytometry and microsatellite markers. The results proved that micropropagation, for purposes of rejuvenation, can be a viable technique to generate genetically stable or identical E. globulus hybrid clones.

Molecular markers based genetic fidelity assessment of micropropagated Dendrobium chrysotoxum Lindl

Meta Gene ◽  
2019 ◽  
Vol 20 ◽  
pp. 100562 ◽  
Author(s):  
Leimapokpam Tikendra ◽  
Arunkumar Singh Koijam ◽  
Potshangbam Nongdam
Keyword(s):  
Molecular Markers ◽  
Genetic Fidelity ◽  
Fidelity Assessment

scholarly journals In Vitro Propagation of an Endangered Helianthus verticillatus by Axillary Bud Proliferation

Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 712
Author(s):  
Marzena Nowakowska ◽  
Žaklina Pavlović ◽  
Marcin Nowicki ◽  
Sarah L. Boggess ◽  
Robert N. Trigiano
Keyword(s):  
Endangered Species ◽  
Shoot Proliferation ◽  
Genetic Fidelity ◽  
Induction Medium ◽  
Axillary Shoot ◽  
Axillary Shoot Proliferation ◽  
Regenerated Plants ◽  
Micropropagated Plants ◽  
Ssrs Markers

Helianthus verticillatus (Asteraceae), whorled sunflower, is a perennial species restricted to a few locations in the Southeastern United States. Habitat loss has caused H. verticillatus to become rare, and since 2014, it has been federally listed as an endangered species. As a part of the recovery plan for the restoration and protection of H. verticillatus, an efficient micropropagation protocol based on axillary shoot proliferation was developed. Various concentrations of 6-benzylaminopurine (BAP; 0 to 4.44 µM) were examined for their morphogenetic potential in the regeneration of six genotypes of H. verticillatus from the nodal explants derived from greenhouse-grown plants. Both the BAP concentration and genotype had significant effects on the regeneration capacity of H. verticillatus. Although the induced buds were observed on ½-strength Murashige and Skoog medium without plant growth regulators, a higher rate of induction and bud development were achieved on media with either 0.88 or 2.22 µM BAP, regardless of the genotype. Successful rooting of the induced shoots was achieved within four weeks after the transfer from the induction medium to the fresh ½-strength MS medium, but the rooting efficiency was dependent on the plant’s genetic background. Regenerated plantlets, with well-developed shoots and roots, were acclimatized successfully to greenhouse conditions with a 97% survival rate. Simple sequence repeats (SSRs) markers were employed to assess the genetic uniformity of the micropropagated plants of H. verticillatus. No extraneous bands were detected between regenerants and their respective donor plants, confirming the genetic fidelity and stability of regenerated plants. To our knowledge, the protocol developed in this study is the first such report for this endangered species.

Rapid regeneration and analysis of genetic fidelity and scopoletin contents of micropropagated plants ofSpilanthes oleraceaL.

2014 ◽  
Vol 89 (1) ◽  
pp. 79-85 ◽  
Author(s):  
V. S. Dandin ◽  
P. M. Naik ◽  
H. N. Murthy ◽  
S. Y. Park ◽  
E. J. Lee ◽  
...  
Keyword(s):  
Genetic Fidelity ◽  
Micropropagated Plants ◽  
Rapid Regeneration

Evaluation of genetic fidelity among micropropagated plants of Salvadora persica L. using DNA-based markers

Meta Gene ◽  
2017 ◽  
Vol 14 ◽  
pp. 129-133 ◽  
Author(s):  
Suman Kumari ◽  
Kuldeep Yadav ◽  
Narender Singh
Keyword(s):  
Genetic Fidelity ◽  
Salvadora Persica ◽  
Micropropagated Plants
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