scholarly journals Identification of Outer Membrane Proteins Altered in Response to UVC-Radiation in Vibrio parahaemolyticus and Vibrio alginolyticus

2012 ◽  
Vol 52 (4) ◽  
pp. 660-665 ◽  
Author(s):  
Fethi Ben Abdallah ◽  
Rihab Lagha ◽  
Ali Ellafi ◽  
Abdelkader Namane ◽  
Jean-Claude Rousselle ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Outer Membrane ◽  
Vibrio Parahaemolyticus ◽  
Outer Membrane Proteins ◽  
Vibrio Alginolyticus ◽  
Uvc Radiation

scholarly journals Identification of outer membrane proteins of Vibrio parahaemolyticus and Vibrio alginolyticus altered in response to γ-irradiation or long-term starvation

2010 ◽  
Vol 161 (10) ◽  
pp. 869-875 ◽  
Author(s):  
Fethi Ben Abdallah ◽  
Ali Ellafi ◽  
Rihab Lagha ◽  
Amina Bakhrouf ◽  
Abdelkader Namane ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Outer Membrane ◽  
Vibrio Parahaemolyticus ◽  
Outer Membrane Proteins ◽  
Vibrio Alginolyticus ◽  
Γ Irradiation

Immunoproteomic Identification of Polyvalent Vaccine Candidates from Vibrio parahaemolyticus Outer Membrane Proteins

2010 ◽  
Vol 9 (5) ◽  
pp. 2573-2583 ◽  
Author(s):  
Hui Li ◽  
Ming-Zhi Ye ◽  
Bo Peng ◽  
Hong-Kai Wu ◽  
Chang-Xin Xu ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Outer Membrane ◽  
Vibrio Parahaemolyticus ◽  
Outer Membrane Proteins ◽  
Vaccine Candidates ◽  
Polyvalent Vaccine

scholarly journals Identification of Vibrio parahaemolyticus and Vibrio spp. Specific Outer Membrane Proteins by Reverse Vaccinology and Surface Proteome

2021 ◽  
Vol 11 ◽  
Author(s):  
Wenbin Wang ◽  
Jianxin Liu ◽  
Shanshan Guo ◽  
Lei Liu ◽  
Qianyun Yuan ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Outer Membrane ◽  
Vibrio Parahaemolyticus ◽  
Outer Membrane Proteins ◽  
Subcellular Location ◽  
Hypothetical Protein ◽  
High Adhesion ◽  
Proteomic Study ◽  
Α Helix ◽  
Surface Proteome

The discovery of outer membrane proteins (OMPs) with desirable specificity and surface availability is a fundamental challenge to develop accurate immunodiagnostic assay and multivalent vaccine of pathogenic Vibrio species in food and aquaculture. Herein 101 OMPs were systemically screened from 4,831 non-redundant proteins of Vibrio parahaemolyticus by bioinformatical predication of signaling peptides, transmembrane (TM) α-helix, and subcellular location. The sequence homology analysis with 32 species of Vibrio spp. and all the non-Vibrio strains revealed that 15 OMPs were conserved in at least 23 Vibrio species, including BamA (VP2310), GspD (VP0133), Tolc (VP0425), OmpK (VP2362), OmpW (VPA0096), LptD (VP0339), Pal (VP1061), flagellar L-ring protein (VP0782), flagellar protein MotY (VP2111), hypothetical protein (VP1713), fimbrial assembly protein (VP2746), VacJ lipoprotein (VP2214), agglutination protein (VP1634), and lipoprotein (VP1267), Chitobiase (VP0755); high adhesion probability of flgH, LptD, OmpK, and OmpW indicated they were potential multivalent Vibrio vaccine candidates. V. parahaemolyticus OMPs were found to share high homology with at least one or two Vibrio species, 19 OMPs including OmpA like protein (VPA073), CsuD (VPA1504), and MtrC (VP1220) were found relatively specific to V. parahaemolyticus. The surface proteomic study by enzymatical shaving the cells showed the capsular polysaccharides most likely limited the protease action, while the glycosidases improved the availability of OMPs to trypsin. The OmpA (VPA1186, VPA0248, VP0764), Omp (VPA0166), OmpU (VP2467), BamA (VP2310), TolC (VP0425), GspD (VP0133), OmpK (VP2362), lpp (VPA1469), Pal (VP1061), agglutination protein (VP1634), and putative iron (III) compound receptor (VPA1435) have better availability on the cell surface.

Proteomic analysis of salt-sensitive outer membrane proteins of Vibrio parahaemolyticus

2004 ◽  
Vol 155 (10) ◽  
pp. 835-842 ◽  
Author(s):  
Changxin Xu ◽  
Haixia Ren ◽  
Sanying Wang ◽  
Xuanxian Peng
Keyword(s):  
Membrane Proteins ◽  
Outer Membrane ◽  
Proteomic Analysis ◽  
Vibrio Parahaemolyticus ◽  
Outer Membrane Proteins

Cloning, expression and immunogenicty analysis of five outer membrane proteins of Vibrio parahaemolyticus zj2003

2007 ◽  
Vol 23 (3) ◽  
pp. 567-575 ◽  
Author(s):  
Zhijuan Mao ◽  
Lian Yu ◽  
Zhenqiang You ◽  
Yongwei Wei ◽  
Yan Liu
Keyword(s):  
Membrane Proteins ◽  
Outer Membrane ◽  
Vibrio Parahaemolyticus ◽  
Outer Membrane Proteins
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