Preparation and characterization of monoclonal antibodies against VP1 protein of foot-and-mouth disease virus O/China99

2009 ◽  
Vol 24 (6) ◽  
pp. 566-572
Author(s):  
Shuai Song ◽  
Tong Lin ◽  
Jun-jun Shao ◽  
Shan-dian Gao ◽  
Guo-zheng Cong ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Vp1 Protein ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Characterization of Foot-and-Mouth Disease Virus by Monoclonal Antibodies

1993 ◽  
Vol 6 (3) ◽  
pp. 219-228 ◽  
Author(s):  
ALBINO ALONSO ◽  
MARIA DA PENHA D. GOMES ◽  
ALBERTO KNUST RAMALHO ◽  
ROSSANA ALLENDE ◽  
HORACIO BARAHONA ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Generation and characterization of genetically stable heterohybridomas producing foot-and-mouth disease virus-specific porcine monoclonal antibodies

2020 ◽  
Vol 487 ◽  
pp. 112873
Author(s):  
Michael C. Puckette ◽  
Erica Martel ◽  
Jacob Rutherford ◽  
José Barrera ◽  
William Hurtle ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Study on the Porcinophilic Foot-and-Mouth Disease Virus I. Production and Characterization of Monoclonal Antibodies against VP1

2006 ◽  
Vol 68 (8) ◽  
pp. 859-864 ◽  
Author(s):  
Ivan-Chen CHENG ◽  
Shu-Mei LIANG ◽  
Wen-Jane TU ◽  
Chi-Min CHEN ◽  
Siou-Yu LAI ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Expression and Immunogenicity of Two Recombinant Fusion Proteins Comprising Foot-and-Mouth Disease Virus Structural Protein VP1 and DC-SIGN-Binding Glycoproteins

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Xinsheng Liu ◽  
Jianliang Lv ◽  
Yuzhen Fang ◽  
Peng Zhou ◽  
Yanzhen Lu ◽  
...  
Keyword(s):  
Immune Responses ◽  
Disease Virus ◽  
Fusion Proteins ◽  
Insect Cells ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Vp1 Protein ◽  
Sf9 Insect Cells ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Improving vaccine immunogenicity by targeting antigens to dendritic cells has recently emerged as a new design strategy in vaccine development. In this study, the VP1 gene of foot-and-mouth disease virus (FMDV) serotype A was fused with the gene encoding human immunodeficiency virus (HIV) membrane glycoprotein gp120 or C2-V3 domain of hepatitis C virus (HCV) envelope glycoprotein E2, both of which are DC-SIGN-binding glycoproteins. After codon optimization, the VP1 protein and the two recombinant VP1-gp120 and VP1-E2 fusion proteins were expressed in Sf9 insect cells using the insect cell-baculovirus expression system. Western blotting showed that the VP1 protein and two recombinant VP1-gp120 and VP1-E2 fusion proteins were correctly expressed in the Sf9 insect cells and had good reactogenicity. Guinea pigs were then immunized with the purified proteins, and the resulting humoral and cellular immune responses were analyzed. The VP1-gp120 and VP1-E2 fusion proteins induced significantly higher specific anti-FMDV antibody levels than the VP1 protein and stronger cell-mediated immune responses. This study provides a new perspective for the development of novel FMDV subunit vaccines.

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