ppGpp-mediated stationary phase induction of the genes encoded by horizontally acquired pathogenicity islands and cob/pdu locus in Salmonella enterica serovar Typhimurium

2010 ◽  
Vol 48 (1) ◽  
pp. 89-95 ◽  
Author(s):  
Miryoung Song ◽  
Hyun-Ju Kim ◽  
Sangryeol Ryu ◽  
Hyunjin Yoon ◽  
Jiae Yun ◽  
...  
Keyword(s):  
Stationary Phase ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Pathogenicity Islands ◽  
Serovar Typhimurium

scholarly journals Salmonella enterica Serovar Typhimurium Induces Cell Death in Bovine Monocyte-Derived Macrophages by Early sipB-Dependent and DelayedsipB-Independent Mechanisms

2001 ◽  
Vol 69 (4) ◽  
pp. 2293-2301 ◽  
Author(s):  
Renato L. Santos ◽  
Renée M. Tsolis ◽  
Andreas J. Bäumler ◽  
Roger Smith ◽  
L. Garry Adams
Keyword(s):  
Cell Death ◽  
Stationary Phase ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Systemic Disease ◽  
Logarithmic Phase ◽  
Intracellular Pathogens ◽  
Caspase Inhibitor ◽  
Serovar Typhimurium ◽  
General Caspase Inhibitor

ABSTRACT It was previously demonstrated that Salmonella enterica serovar Typhimurium induces cell death with features of apoptosis in murine macrophages. Mice infected withSalmonella serovar Typhimurium develop systemic disease without diarrhea, whereas the infection in cattle and in humans is localized and characterized by diarrhea. Considering these clinical disease expression differences between mice and cattle, we investigated whether serovar Typhimurium is cytotoxic for bovine macrophages. Macrophages infected with serovar Typhimurium grown in the logarithmic phase quickly underwent cell death. Macrophages infected with stationary-phase cultures or with a mutant lacking sipBunderwent no immediate cell death but did develop delayed cytotoxicity, undergoing cell death between 12 and 18 h postinfection. Both pathways were temporarily blocked by the general caspase inhibitor Z-VAD-Fmk and by the caspase 1 inhibitor Z-YVAD-Fmk. Comparisons of macrophages from cattle naturally resistant or susceptible to intracellular pathogens indicated no differences between these two genetic backgrounds in terms of susceptibility to serovar Typhimurium-induced cell death. We conclude thatSalmonella serovar Typhimurium induces cell death in bovine macrophages by two distinct mechanisms, earlysipB-mediated and delayedsipB-independent mechanisms.

scholarly journals Selective Capture of Salmonella enterica Serovar Typhi Genes Expressed in Macrophages That Are Absent from the Salmonella enterica Serovar Typhimurium Genome

2005 ◽  
Vol 73 (8) ◽  
pp. 5217-5221 ◽  
Author(s):  
Sébastien P. Faucher ◽  
Roy Curtiss ◽  
France Daigle
Keyword(s):  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Pathogenicity Islands ◽  
Human Macrophages ◽  
Salmonella Enterica Serovar Typhi ◽  
Serovar Typhimurium ◽  
Transcribed Sequences

ABSTRACT Thirty-six Salmonella enterica serovar Typhi-specific genes, absent from the Salmonella enterica serovar Typhimurium genome, that were expressed in human macrophages were identified by selective capture of transcribed sequences. These genes are located on 15 unique loci of the serovar Typhi genome, including Salmonella pathogenicity islands (SPI-7, SPI-8, and SPI-10) and bacteriophages (ST15, ST18, and ST35).

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