VP2 gene sequencing based Geno-grouping of infectious bursal disease viruses isolated from Gujarat and Maharashtra state (India)

VirusDisease ◽  
2021 ◽  
Author(s):  
Ritesh S. Shinde ◽  
Harshadkumar C. Chauhan ◽  
Arun C. Patel ◽  
Kishan K. Sharma ◽  
Sandip S. Patel ◽  
...  
2001 ◽  
Vol 30 (5) ◽  
pp. 501-507 ◽  
Author(s):  
R. S. Kataria ◽  
A. K. Tiwari ◽  
G. Butchaiah ◽  
J. M. Kataria ◽  
M. A. Skinner

2012 ◽  
Vol 48 (No. 12) ◽  
pp. 359-362 ◽  
Author(s):  
G. Ozbes ◽  
Ertas HB ◽  
A. Muzo

Infectious bursal disease Virus (IBDV) specific reverse transcriptase/polymerase chain recation (RT/PCR) positive 40 broiler bursa fabricius samples obtained from a commercially reared flock were investigated for genetic diversity by PCR-RFLP assay. The assay amplifies a 743 bp fragment of the IBDV VP2 gene. The RFLP profiles of 40 of these positive samples were determined using the enzyme MboI. Most of the viruses had the same RFLP with the MboI enzyme. RFLP analysis of the isolates produced two different band profiles. The results of this study showed that little genetic heterogeneity exists among IBDV strains in a infected flock.


Author(s):  
Phạm Hồng Sơn ◽  
Phạm Hồng Kỳ ◽  
Nguyễn Thị Lan Hương ◽  
Phạm Thị Hồng Hà

. Using the method of shifting assay of standardized indirect agglutination (SSIA), the prevalence of Newcastle disease viruses (NDV) and infectious bursal disease viruses (IBDV) in chickens reared in several districts of Thua Thien Hue province in the Spring-Summer and Fall-Winter seasons was determined. In the Spring-Summer season of 2011, about 22.3% of the chickens were infected with NDV, in which A Luoi  accounted for the highest percentage of 25% of the infected chickens and Huong Thuy  the lowest  of 18.2%. Meanwhile, 36% of the same chickens were infected with IBDV, with the highest percentage (46.66%) also in A Luoi and the lowest (30.3%) also in Huong Thuy. The intensity of NDV infection in the Spring-Summer season in A Luoi and Phu Vang was highest (GMT = 1.45), and in Huong Thuy lowest (GMT = 1.31). In addition, in the Fall-Winter season, about 46% of the chickens were infected with NDV and 46.3% with IBDV in Huong Thuy and Phu Vang – two neighbouring districts of Hue City, in which NDV was detected in 54.4% of the chickens in Huong Thuy and 33.9% in Phu Vang. In contrast, IBDV was detected in 41.9% and 52.7% of the chickens respectively in the two districts. The infection was not inter-dependent. Methodically, although the differences in the infection rates were insignificant with the accuracy of 95%, faecal samples showed higher sensitivity in SSIA analyses for both cases of NDV and IBDV infection in comparision with mouth exudates. By SSIA method, results could be read clearly with unaided eyes for a long time after the performance, and it was also proven applicable for cases of haemagglutinating viruses if proper treatments for depletion of animal RBCs’ surface agglutinins could be applied.


2000 ◽  
Vol 44 (3) ◽  
pp. 691 ◽  
Author(s):  
Hyuk Moo Kwon ◽  
Dae Kyu Kim ◽  
Tae Wook Hahn ◽  
Jeong Hee Han ◽  
Daral J. Jackwood

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