A continuous fluorescence assay for cyclic nucleotide phosphodiesterase hydrolysis of cyclic GMP

1987 ◽  
Vol 162 (1) ◽  
pp. 291-295 ◽  
Author(s):  
J.David Johnson ◽  
John D. Walters ◽  
John S. Mills
Keyword(s):  
Cyclic Gmp ◽  
Cyclic Nucleotide ◽  
Fluorescence Assay ◽  
Cyclic Nucleotide Phosphodiesterase ◽  
Hydrolysis Of

scholarly journals Complex effects of inhibitors on cyclic GMP-stimulated cyclic nucleotide phosphodiesterase.

1983 ◽  
Vol 258 (23) ◽  
pp. 14173-14177 ◽  
Author(s):  
T Yamamoto ◽  
S Yamamoto ◽  
J C Osborne ◽  
V C Manganiello ◽  
M Vaughan ◽  
...  
Keyword(s):  
Cyclic Gmp ◽  
Cyclic Nucleotide ◽  
Cyclic Nucleotide Phosphodiesterase

Amino acid sequence of the cyclic GMP stimulated cyclic nucleotide phosphodiesterase from bovine heart

Biochemistry ◽  
1990 ◽  
Vol 29 (44) ◽  
pp. 10280-10288 ◽  
Author(s):  
Hai Le Trong ◽  
Norbert Beier ◽  
William K. Sonnenburg ◽  
Steven D. Stroop ◽  
Kenneth A. Walsh ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Cyclic Gmp ◽  
Cyclic Nucleotide ◽  
Cyclic Nucleotide Phosphodiesterase ◽  
Bovine Heart

Influence of dopamine on cyclic nucleotide enzymes in bovine retinal membrane fractions

1993 ◽  
Vol 10 (6) ◽  
pp. 991-996 ◽  
Author(s):  
Ari Sitaramayya ◽  
Lorraine Lombardi ◽  
Alexander Margulis
Keyword(s):  
Cyclic Amp ◽  
Cyclic Gmp ◽  
Cyclic Nucleotide ◽  
D2 Receptors ◽  
D1 Receptors ◽  
Metabolizing Enzymes ◽  
D2 Agonist ◽  
Hydrolysis Of ◽  
Cyclic Amp Synthesis

AbstractDopamine is a major neurotransmitter and neuromodulator in vertebrate retina. Although its pharmacological and physiological actions are well understood, the biochemical mechanisms of its signal transduction are less clear. Acting via D1 receptors, dopamine was shown to increase cyclic AMP levels in intact retina and to activate adenylate cyclase in retinal homogenates. The action via activation of D2 receptors is controversial: it was reported to decrease cyclic AMP levels in intact retina but inhibition of cyclase could not be demonstrated in retinal homogenates; also it was reported to activate rod outer segment cyclic GMP phosphodiesterase in vitro but did not decrease cyclic GMP levels in aspartate-treated retinas. We made an attempt to fractionate bovine retinal membranes and to investigate the effects of dopamine, via Dl and D2 receptors, on the synthesis and hydrolysis of cyclic AMP and cyclic GMP. Activation of cyclic AMP synthesis was noted in all fractions, but no effects were evident on cyclic nucleotide hydrolysis or cyclic GMP synthesis in any fraction. Also, D2 agonist did not inhibit cyclic AMP synthesis. These observations suggest that D2 receptors may not be directly coupled to cyclic nucleotide metabolizing enzymes in bovine retina.

scholarly journals Insulin stimulates hormone-sensitive cyclic GMP-inhibited cyclic nucleotide phosphodiesterase in rat brown adipose cells

FEBS Letters ◽  
1995 ◽  
Vol 374 (2) ◽  
pp. 187-191 ◽  
Author(s):  
Mariko Omatsu-Kanbe ◽  
Samuel W. Cushman ◽  
Vincent C. Manganiello ◽  
Masato Taira
Keyword(s):  
Cyclic Gmp ◽  
Cyclic Nucleotide ◽  
Cyclic Nucleotide Phosphodiesterase ◽  
Brown Adipose ◽  
Hormone Sensitive ◽  
Adipose Cells
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