The effects of carbon tetrachloride and ethionine on RNA synthesis in vivo and in isolated rat liver nuclei

1969 ◽  
Vol 132 (1) ◽  
pp. 62-79 ◽  
Author(s):  
Edward A. Smuckler ◽  
Marlene Koplitz
Keyword(s):  
Carbon Tetrachloride ◽  
Rat Liver ◽  
Rna Synthesis ◽  
Isolated Rat Liver ◽  
Rat Liver Nuclei ◽  
Liver Nuclei ◽  
Isolated Rat

scholarly journals Modulation by Exogenous Histones of Phosphorylation of Non-Histone Nuclear Proteins in Isolated Rat Liver Nuclei

1973 ◽  
Vol 248 (21) ◽  
pp. 7595-7600
Author(s):  
Edward M. Johnson ◽  
Giorgio Vidali ◽  
Virginia C. Littau ◽  
Vincent G. Allfrey
Keyword(s):  
Rat Liver ◽  
Nuclear Proteins ◽  
Isolated Rat Liver ◽  
Rat Liver Nuclei ◽  
Liver Nuclei ◽  
Isolated Rat

scholarly journals Cytochemical studies on the relation of nucleoside triphosphatase activity to ribonucleoproteins in isolated rat liver nuclei.

1980 ◽  
Vol 28 (1) ◽  
pp. 27-35 ◽  
Author(s):  
A Vorbrodt ◽  
G G Maul
Keyword(s):  
Nuclear Envelope ◽  
Rat Liver ◽  
Enzyme Reaction ◽  
Point Of View ◽  
Nuclear Pore ◽  
Nuclear Pore Complexes ◽  
Isolated Rat Liver ◽  
Rat Liver Nuclei ◽  
Liver Nuclei ◽  
Isolated Rat

Cytochemical tests for nucleosidetriphosphatase (NTPase) and Bernhard's preferential staining for ribonucleoproteins (RNP) were applied to isolated rat liver nuclei. The strongest and most easily reproducible positive reaction for NTPase was detected at pH 7.7 with ATP and GTP. This reaction was activated by Mg2+ and Ca2+ and inhibited by Be2+, Zn2+, quercetin, and ribonuclease. The major sites of enzyme reaction were intranuclear RNA-containing structures. Incubation of nuclei in ATP-stimulated RNA-release medium eliminated a considerable part of the material showing both NTPase reaction and staining for RNP; the perichromatin granules disappeared, while interchromatin granules remained. NTPase activity in the nuclear envelope seems to be associated with the annular part of nuclear pore complexes (permanent component) and with RNP particles translocated through nuclear pores or attached to the surface of nuclei (transitional component). From a morphological point of view, these observations support previous biochemical data suggesting the existence of a connection between NTPase activity and the translocation of RNP particles through the nuclear envelope.

scholarly journals CYTOFLUOROMETRIC DETERMINATION OF LYSINE WITH DANSYLCHLORIDE

1968 ◽  
Vol 16 (7) ◽  
pp. 459-466 ◽  
Author(s):  
A. ROSSELET ◽  
F. RUCH
Keyword(s):  
Rat Liver ◽  
Lysine Content ◽  
Amino Groups ◽  
Isolated Rat Liver ◽  
Model Experiments ◽  
Rat Liver Nuclei ◽  
Liver Nuclei ◽  
Isolated Rat

Dansylchloride (l-dimethylamino-naphthalene-5-sulfochloride) may be used for cytofluorometric determination of lysine. By means of model experiments on protein smears it is shown that the reaction must be carried out in ethanol if it is to be specific for amino groups. The fluorescence given by isolated rat liver nuclei treated with dansylchloride corresponds to the three classes of 2n, 4n and 8n nuclei. The dansylfluorescence of several kinds of sperms is proportional to their lysine content. In rat liver nuclei, 95% of the lysine is dansylated and the lysine content may be determined in absolute values by comparison with polylysine. In spermatozoa only 50% of the lysine reacts.

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