Enrichment and biochemical characterization of boundary membrane contact sites from rat-liver mitochondria

1986 ◽  
Vol 860 (3) ◽  
pp. 672-689 ◽  
Author(s):  
Kay Ohlendieck ◽  
Ingrid Riesinger ◽  
Volker Adams ◽  
Jürgen Krause ◽  
Dieter Brdiczka
Keyword(s):  
Rat Liver ◽  
Biochemical Characterization ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Membrane Contact Sites ◽  
Contact Sites ◽  
Membrane Contact ◽  
Boundary Membrane

The ERMES complex and ER–mitochondria connections

2012 ◽  
Vol 40 (2) ◽  
pp. 445-450 ◽  
Author(s):  
Agnès H. Michel ◽  
Benoît Kornmann
Keyword(s):  
Protein Complex ◽  
Biochemical Characterization ◽  
Eukaryotic Cell ◽  
Yeast Protein ◽  
Membrane Contact Sites ◽  
Contact Sites ◽  
Synthetic Protein ◽  
Membrane Contact ◽  
Cellular Organelles

Cellular organelles need to communicate in order to co-ordinate homoeostasis of the compartmentalized eukaryotic cell. Such communication involves the formation of membrane contact sites between adjacent organelles, allowing privileged exchange of metabolites and information. Using a synthetic protein designed to artificially tether the ER (endoplasmic reticulum) to mitochondria, we have discovered a yeast protein complex naturally involved in establishing and maintaining contact sites between these two organelles. This protein complex is physiologically involved in a plethora of mitochondrial processes, suggesting that ER–mitochondria connections play a central co-ordinating role in the regulation of mitochondrial biology. Recent biochemical characterization of this protein complex led to the discovery that GTPases of the Miro family are part of ER–mitochondria connections. The yeast Miro GTPase Gem1 localizes to ER–mitochondria interface and influences the size and distribution of mitochondria. Thus Miro GTPases may serve as regulators of the ER–mitochondria connection.

scholarly journals Characterization of a DNA primase from rat liver mitochondria.

1986 ◽  
Vol 261 (14) ◽  
pp. 6571-6577
Author(s):  
B J Ledwith ◽  
S Manam ◽  
G C Van Tuyle
Keyword(s):  
Rat Liver ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Dna Primase

Characterization of betaine efflux from rat liver mitochondria

1993 ◽  
Vol 1141 (2-3) ◽  
pp. 269-274 ◽  
Author(s):  
Richard K. Porter ◽  
John M. Scott ◽  
Martin D. Brand
Keyword(s):  
Rat Liver ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria
Sign in / Sign up

Export Citation Format

Share Document