Stimulation and inhibition of polypeptide synthesis by streptomycin in ribosomal systems of Escherichia coli, programmed with various messengers

1965 ◽  
Vol 95 (3) ◽  
pp. 461-473 ◽  
Author(s):  
P.H. Van Knippenberg ◽  
J.C.Van Ravenswaay Claasen ◽  
M. Grijm-Vos ◽  
H. Veldstra ◽  
L. Bosch
Keyword(s):  
Escherichia Coli ◽  
Polypeptide Synthesis

scholarly journals Effects of various triamines on cell-free polypeptide synthesis of Escherichia coli and on growth of its polyamine auxotrophs.

1990 ◽  
Vol 38 (6) ◽  
pp. 1648-1652 ◽  
Author(s):  
Yasuyoshi KOUMOTO ◽  
Yumiko HISAMOTO ◽  
Sumio SHINODA ◽  
Shigeo YAMAMOTO
Keyword(s):  
Escherichia Coli ◽  
Polypeptide Synthesis

Inhibition by aminochromes of in vitro polypeptide synthesis in Escherichia coli

1969 ◽  
Vol 134 (2) ◽  
pp. 478-485 ◽  
Author(s):  
David Shafritz ◽  
Frederick Goodwin ◽  
Herbert Weissbach
Keyword(s):  
Escherichia Coli ◽  
Polypeptide Synthesis

scholarly journals The effect of hypermethylation on the functional properties of transfer ribonucleic acid. Ribosome-binding and polypeptide synthesis

1970 ◽  
Vol 119 (3) ◽  
pp. 587-593 ◽  
Author(s):  
J. Hay ◽  
D. J. Pillinger ◽  
E. Borek
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Binding Assay ◽  
Covalent Bond ◽  
Methyl Groups ◽  
Ribosome Binding ◽  
Phosphodiester Bonds ◽  
Polypeptide Synthesis ◽  
The Stability ◽  
Escherichia Coli B

1. Phenylalanyl-tRNA formed after chemical hypermethylation of Escherichia coli B tRNA was able to bind to ribosomes with the same efficiency as normal phenylalanyl-tRNA. 2. Under incubation conditions used in the ribosome-binding assay, hypermethylation of tRNA did not measurably decrease the stability of either inter-nucleotide phosphodiester bonds or the covalent bond between amino acid and tRNA in phenylalanyl-tRNA. 3. The ability of hypermethylated tRNA to take part in polyphenylalanine synthesis was inhibited progressively as the degree of hypermethylation increased. 4. Hypermethylation of tRNA affected polyphenylalanine synthesis at the stage of amino acid recognition and at a further point in the synthesis but not at the level of codon–anticodon recognition. 5. The formation of polylysine was more seriously affected by hypermethylation of tRNA than would be accounted for by inhibition of amino acid acceptance alone. 6. Polyproline formation was completely inhibited by the presence of 7mol% excess of methyl groups in tRNA. 7. The possibility of a link between amino acid acceptance and ribosome-binding was suggested for phenylalanyl-tRNA, but not for lysyl- or prolyl-tRNA.

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