Suppression of experimental autoimmune thyroiditis in the guinea pig by pretreatment with thyroglobulin in incomplete Freund's adjuvant

1978 ◽  
Vol 39 (2) ◽  
pp. 289-296 ◽  
Author(s):  
Helen Braley-Mullen ◽  
Gordon C. Sharp ◽  
Michael Kyriakos ◽  
Nancy Hayes ◽  
Catherine Dunn ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Autoimmune Thyroiditis ◽  
Experimental Autoimmune Thyroiditis ◽  
Incomplete Freund’S Adjuvant ◽  
Freund’S Adjuvant ◽  
Freund's Adjuvant ◽  
Experimental Autoimmune

scholarly journals Iodine Intake Increases IP-10 Expression in the Serum and Thyroids of Rats with Experimental Autoimmune Thyroiditis

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Si-lu Cui ◽  
Jun Yu ◽  
Liu Shoujun
Keyword(s):  
Autoimmune Thyroiditis ◽  
Thyroid Tissue ◽  
Iodine Concentration ◽  
Iodine Intake ◽  
Experimental Autoimmune Thyroiditis ◽  
High Iodine ◽  
Incomplete Freund’S Adjuvant ◽  
Iodine Treatment ◽  
Inducible Protein ◽  
Experimental Autoimmune

Here, we sought to establish an experimental autoimmune thyroiditis rat model induced by bovine thyroglobulin (bTg) injection and to investigate pathological changes and variations in serum interferon-γ-inducible protein of 10 kDa (IP-10) in thyroid tissue following iodine treatment. Four-week-old female Lewis rats(n=135)were randomly divided into normal (NC), thyroglobulin (TG), HI, HI+TG, HII, and HII+TG groups; rats in the NC and TG groups drank only distilled water (iodine concentration: 10 μg/L), rats in the HI and HI+TG groups were given water containing 25.7 mg/L iodine, and rats in the HII and HII+TG groups were given water containing 423.3 mg/L iodine. Rats in the TG, HI+TG, and HII+TG groups were immunized with 0.1 mL bTg (8 mg/mL) in incomplete Freund’s adjuvant once every 2 weeks for 6 weeks. Compared with the NC group, the TG, HI+TG, and HII+TG groups exhibited higher iodine intake and increased thyroid weights with increasing iodine doses(P<0.05). The high iodine intake in the TG group was associated with increased CD4+T cells and serum IP-10. Thus, high iodine consumption aggravated the inflammatory reaction in the thyroid and mild high iodine consumption increased serum IP-10 levels after induction with bTg.

scholarly journals TRANSFER OF EXPERIMENTAL AUTOIMMUNE THYROIDITIS BY SERUM FROM THYROIDECTOMIZED DONORS

1969 ◽  
Vol 130 (2) ◽  
pp. 263-285 ◽  
Author(s):  
R. M. Nakamura ◽  
W. O. Weigle
Keyword(s):  
Guinea Pig ◽  
Autoimmune Thyroiditis ◽  
Guinea Pigs ◽  
Globulin Fraction ◽  
Experimental Autoimmune Thyroiditis ◽  
Antithyroglobulin Antibody ◽  
Thyroid Glands ◽  
Successful Transfer ◽  
Thyroid Lesions ◽  
Experimental Autoimmune

When rabbits were injected with 10.0 mg rabbit thyroglobulin in complete Freund's adjuvant, the earliest thyroid lesions were seen on day 5 and uniformly severe thyroid lesions were seen by day 14; these observations were not significantly different from the thyroid lesions observed at 1 and 2 months post-immunization. Pooled sera were obtained from immunized, thyroidectomized, and nonthyroidectomized donors on various days and transferred to normal recipient rabbits in different experiments. Successful transfer of thyroid lesions was seen when serum containing early antithyroglobulin antibody obtained from thyroidectomized donor animals at various times after immunization was injected into normal recipients in a sequential manner. Immunofluorescent studies of recipient thyroid glands showed focal fixation of rabbit γ-globulin and ß1C complement in thyroid follicles. When purified antibody to rabbit thyroglobulin obtained from thyroidectomized donor sera was transferred sequentially as above, significant thyroid lesions were seen in recipient rabbits. In contrast, no thyroid lesions were seen in recipient animals injected with rabbit sera containing late antithyroglobulin antibody from thyroidectomized donors or hyperimmune sera from guinea pigs. No thyroid lesions were seen in recipient animals injected either with sera from donors given complete adjuvant without thyroglobulin or with globulin fraction of pooled sera containing early antithyroglobulin antibody obtained on various days from nonthyroidectomized donors. Similarly, rabbits rendered unresponsive to guinea pig γG-globulin and periodically injected with guinea pig anti-rabbit thyroglobulin showed no thyroid lesions.

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