A new look at fluorescence depolarization and the dynamics of anisotropic rotational diffusion

1987 ◽  
Vol 136 (5) ◽  
pp. 407-412 ◽  
Author(s):  
K. Razi Naqvi
Keyword(s):  
Rotational Diffusion ◽  
Fluorescence Depolarization ◽  
Anisotropic Rotational Diffusion ◽  
New Look

scholarly journals Size and Shape of Human Fibrinogen in Solution

1977 ◽  
Author(s):  
V. Hofmann ◽  
P.W. Straub ◽  
T. Binkert ◽  
E. Serrallach ◽  
W. Känzig ◽  
...  
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Analytical Ultracentrifugation ◽  
Rotational Diffusion ◽  
Axial Ratio ◽  
Major Axis ◽  
Fluorescence Depolarization ◽  
Human Fibrinogen ◽  
Size And Shape ◽  
Rotational Diffusion Coefficients

In order to obtain information on size and shape of the fibrinogen molecule in solution the translational diffusion coefficient (DT), the rotational diffusion coefficients (DR⊥ and DR//) and the sedimentation coefficients (S) have been measured on human fibrinogen with a clottaoility above 95%. The methods used were dynamic light scattering, nanosecond fluorescence depolarization and analytical ultracentrifugation. Dynamic light scattering yields DT = 2.0 ± 3% x 10-7 cm2sec–1 at a concentration of 7 mg/ml in 0.15 M Tris-NaCl, pH 7.4. DT is strongly dependent on concentration, being 3.4 ± 10% × 10-7 cm2 sec-1 at 0.1 mg/ml. The rotation along the minor axis as measured with the same method is DR = // 1.5 × 106 sec-1 at 0.1 mg/ml. The rotation along the major axis as measured on fibrinogen labeled with dansylchloride is DR// = 1.5 x106 sec–1. S is also strongly dependent on concentration, being 7.9 S at 0.1 mg/ml, 8.1 S at 1 mg/ml and 6.6 S at 10 mg/ml.These results fit with an elongated molecule having an axial ratio of 7. They are compatible with a MW of 340’000 only for concentrations above 2 mg/ml, while at lower concentrations (0.1 mg/ml) they agree with a MW of approximately half the accepted value.

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